Please use this identifier to cite or link to this item: http://repositorio.ufpa.br:8080/jspui/handle/2011/10541
Title: Efeito protetor de antioxidantes na formação de metemoglobina induzida pelo metabólito dapsona-hidroxilamina in vitro
metadata.dc.creator: VARELA, Everton Luiz Pompeu
metadata.dc.contributor.advisor1: MONTEIRO, Marta Chagas
Keywords: Antioxidantes
Dapsona-hidroxilamina
Ebselen
Glutationa
Metemoglobina
Hanseníase
Azul de Metileno
Medicamentos
Issue Date: 11-Jul-2017
Publisher: Universidade Federal do Pará
Citation: VARELA, Everton Luiz Pompeu. Efeito protetor de antioxidantes na formação de metemoglobina induzida pelo metabólito dapsona-hidroxilamina in vitro. Orientadora: Marta Chagas Monteiro. 2017. 116 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, 2017. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/10541. Acesso em:.
Abstract: Dapsone used in leprosy therapy its metabolite dapsone-hydroxylamine are potent pro-oxidant agents that cause acquired methemoglobinemia. For the treatment of this disease is used as antidote the Methylene Blue, however in high doses this antidote becomes pro-oxidant. In this sense, antioxidant substances may be potential alternatives to methylene blue for the treatment of methemoglobinemia. In this study we investigated the effect of antioxidants Ebselen, N-acetylcysteine, R-lipoic acid and L-lipoic acid on oxidative damage induced by dapsone-hydroxylamine in human erythrocytes, in vitro. Our results demonstrated that pre-treatment with antioxidants Ebselen, N-acetylcysteine, R-lipoic acid and S-lipoic acid prevented the formation of methemoglobin, reduction of glutathione and lipid peroxidation induced by the metabolite dapsone-hydroxylamine in human erythrocytes, In vitro. These substances were able to increase the antioxidant capacity of the erythrocyte associated with increased concentration of glutathione. Thus, antioxidants acted to reduce the oxidation of hemoglobin and / or directly or indirectly impeded the action of the metabolite dapsone-hydroxylamine. Our results indicate that the antioxidants tested can protect erythrocytes against oxidative damage under experimental conditions, suggesting that antioxidants may serve as the most effective and safe antidote in the treatment of methemoglobinemia.
URI: http://repositorio.ufpa.br/jspui/handle/2011/10541
Appears in Collections:Dissertações em Ciências Farmacêuticas (Mestrado) - PPGCF/ICS

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