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dc.creatorCHRISTOFOLETTI, Cintya Aparecida-
dc.creatorDAVID, José Augusto de Oliveira-
dc.creatorFONTANETTI, Carmem Silvia-
dc.identifier.citationCHRISTOFOLETTI, Cintya Aparecida; DAVID, José Augusto Oliveira; FONTANETTI, Carmem Silvia. Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison. Genetics and Molecular Biology, São Paulo, v. 32, n. 1, p. 155-158, 2009. Disponível em: <>. Acesso em: 8 set. 2015. <>.pt_BR
dc.description.abstractThe present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectOreochromis niloticuspt_BR
dc.subjectEnsaio cometapt_BR
dc.subjectImpregnação pela pratapt_BR
dc.titleApplication of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparisonpt_BR
dc.typeArtigo de Periódicopt_BR
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