Please use this identifier to cite or link to this item: http://repositorio.ufpa.br/jspui/handle/2011/7003
metadata.dc.type: Artigo de Periódico
Issue Date: Jul-2014
metadata.dc.creator: LIDANI, Kárita Cláudia Freitas
REASON, Iara José de Messias
ISHIKAWA, Edna Aoba Yassui
Title: A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum
Citation: LIDANI, Kárita Cláudia Freitas; REASON, Iara José de Messias; ISHIKAWA, Edna Aoba Yassui. A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 109, n. 4, p. 442-447, jul. 2014. Disponível em: <http://www.scielo.br/pdf/mioc/v109n4/0074-0276-mioc-0074-0276130285.pdf>. Acesso em: 16 nov. 2015. <http://dx.doi.org/10.1590/0074-0276130285>.
Abstract: The aim of the present study was to detect natural infection by Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in Barcarena, state of Pará, Brazil, through the use of three primer sets. With this approach, it is unnecessary to previously dissect the sandfly specimens. DNA of 280 Lu. longipalpis female specimens were extracted from the whole insects. PCR primers for kinetoplast minicircle DNA (kDNA), the mini-exon gene and the small subunit ribosomal RNA (SSU-rRNA) gene of Leishmania were used, generating fragments of 400 bp, 780 bp and 603 bp, respectively. Infection by the parasite was found with the kDNA primer in 8.6% of the cases, with the mini-exon gene primer in 7.1% of the cases and with the SSU-rRNA gene primer in 5.3% of the cases. These data show the importance of polymerase chain reaction as a tool for investigating the molecular epidemiology of visceral leishmaniasis by estimating the risk of disease transmission in endemic areas, with the kDNA primer representing the most reliable marker for the parasite.
Keywords: Leishmaniose visceral
Leishmania infantum
Lutzomyia longipalpis
Barcarena - PA
Pará - Estado
Amazônia brasileira
ISSN: 0074-0276
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - NMT

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