Please use this identifier to cite or link to this item: http://repositorio.ufpa.br:8080/jspui/handle/2011/7024
Full metadata record
DC FieldValueLanguage
dc.creatorSOARES, Vítor Yamashiro Rocha-
dc.creatorSILVA, Jailthon Carlos da-
dc.creatorSILVA, Kleverton Ribeiro da-
dc.creatorCRUZ, Maria do Socorro Pires e-
dc.creatorSANTOS, Marcos Pérsio Dantas-
dc.creatorRIBOLLA, Paulo Eduardo Martins-
dc.creatorALONSO, Diego Peres-
dc.creatorCOELHO, Luiz Felipe Leomil-
dc.creatorCOSTA, Dorcas Lamounier-
dc.creatorCOSTA, Carlos Henrique Nery-
dc.date.accessioned2015-11-25T16:06:55Z-
dc.date.available2015-11-25T16:06:55Z-
dc.date.issued2014-05-
dc.identifier.citationSOARES, Vítor Yamashiro Rocha et al. Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 109, n. 3, p. 379-383, maio 2014. Disponível em: <http://www.scielo.br/pdf/mioc/v109n3/0074-0276-mioc-0074-0276130405.pdf>. Acesso em: 16 nov. 2015. <http://dx.doi.org/10.1590/0074-0276130405>.pt_BR
dc.identifier.issn0074-0276-
dc.identifier.urihttp://repositorio.ufpa.br/jspui/handle/2011/7024-
dc.description.abstractAn analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. The present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial cytochrome B (cytb)gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. The complete cytb gene sequences of 11 vertebrate species available in the National Center for Biotechnology Information database were digested with Aci I, Alu I, Hae III and Rsa I restriction enzymes in silico using Restriction Mapper software. The cytb gene fragment (358 bp) was amplified from tissue samples of vertebrate species and the dietary contents of sandflies and digested with restriction enzymes. Vertebrate species presented a restriction fragment profile that differed from that of other species, with the exception of Canis familiaris and Cerdocyon thous. The 358 bp fragment was identified in 76 sandflies. Of these, 10 were evaluated using the restriction enzymes and the food sources were predicted for four: Homo sapiens (1), Bos taurus (1) and Equus caballus (2). Thus, the PCR-RFLP technique could be a potential method for identifying the food sources of arthropods. However, some points must be clarified regarding the applicability of the method, such as the extent of DNA degradation through intestinal digestion, the potential for multiple sources of blood meals and the need for greater knowledge regarding intraspecific variations in mtDNA.pt_BR
dc.language.isoengpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectInsetospt_BR
dc.subjectReação em cadeia da polimerasept_BR
dc.subjectCitocromos bpt_BR
dc.subjectAnálise de farinha de sanguept_BR
dc.titleIdentification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B genept_BR
dc.typeArtigo de Periódicopt_BR
Appears in Collections:Artigos Científicos - ICB

Files in This Item:
File Description SizeFormat 
Artigo_IdentificationBloodMeal.pdf789,84 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons