Please use this identifier to cite or link to this item: http://repositorio.ufpa.br/jspui/handle/2011/8103
metadata.dc.type: Artigo de Periódico
Issue Date: Jun-2015
metadata.dc.creator: LIMA, Eleonidas Moura
LOPES, Otávio Sérgio
SOARES, Leonardo Ferreira
ARRUDA, Talitta Dantas
GIGEK, Carolina Oliveira
MELO, Cynthia Germoglio Farias
SMITH, Marilia Arruda Cardoso
OLIVEIRA, João Ricardo Gonçalves
MEDEIROS, Arnaldo Correia de
DELATORRE, Plínio
RODRÍGUEZ BURBANO, Rommel Mario
Title: Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
Citation: LIMA, Eleonidas Moura et al. Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic. Brazilian Archives of Biology and Technology, Curitiba, v. 58, n. 3, p. 414-420, mai./jun. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414&lng=pt&nrm=iso>. Acesso em: 06 abr. 2017. <http://dx.doi.org/10.1590/S1516-8913201500434>.
Abstract: Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ 2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ 2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.
Keywords: Câncer gástrico
Genótipo
Polimofismo de nucleotídeo
Oncogenética
Carcinoma gástrico
metadata.dc.subject.cnpq: CNPQ::CIENCIAS EXATAS E DA TERRA
Series/Report no.: Brazilian Archives of Biology and Technology
ISSN: 1678-4324
metadata.dc.publisher.country: Brasil
Publisher: Universidade Federal do Pará
metadata.dc.publisher.initials: UFPA
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - ICB

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