Please use this identifier to cite or link to this item: http://repositorio.ufpa.br/jspui/handle/2011/8889
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Type: Artigo de Periódico
Issue Date: Nov-2006
Authors: MADRUGA, Cláudio Roberto
ARAÚJO, Flábio Ribeiro de
CAVALCANTE, Gustavo Góes
MARTINS, Charles Ferreira
BARBOSA, Imke Barbara Pfeifer
RIBEIRO, Laura Rios
KESSLER, Raul Henrique
SOARES, Cleber Oliveira
MIGUITA, Midori
MELO, Elaine Silva de Pádua
ALMEIDA, Robson Ferreira Cavalcante de
LIMA JUNIOR, Manoel Sebastião da Costa
Title: The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
Citation: MADRUGA, Cláudio Roberto et al. The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 7, p. 801-807, nov. 2006. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700016&lng=pt&nrm=iso>. Acesso em: 21 jul. 2017. <http://dx.doi.org/10.1590/S0074-02762006000700016>.
Abstract: There are data indicating that the distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle herds. For this reason, an enzyme-linked immunosorbent assay (Tv-ELISA-Ab) with crude antigen from one Brazilian isolate of T. vivax was developed and evaluated. The sensitivity and specificity were respectively 97.6 and 96.9%. In the evaluation of cross-reactions, three calves inoculated with T. evansi trypimastigotes blood forms showed optical densities (OD) under the cut-off during the whole experimental period, except one at 45 days post-inoculation. With relation to Babesia bovis, B. bigemina, and Anaplasma marginale, which are endemic hemoparasites in the studied area, the cross-reactions were shown to be 5.7, 5.3, and 1.1%, respectively. The first serological survey of Pantanal and state of Pará showed that T. vivax is widespread, although regions within both areas had significantly different prevalences. Therefore, this Tv-ELISA-Ab may be a more appropriate test for epidemiological studies in developing countries because the diagnostic laboratories in most countries may be able to perform an ELISA, which is not true for polymerase chain reaction.
Keywords: Enzimas
Trypanosoma vivax
Pesquisas epidemiológicas
Anticorpos
Epistemologia
metadata.dc.relation.ispartof: Memórias do Instituto Oswaldo Cruz
ISSN: 1678-8060
Country: Brasil
Publisher: Universidade Federal do Pará
Institution Acronym: UFPA
Appears in Collections:Artigos Científicos - CCAST

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