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metadata.dc.type: Artigo de Periódico
Issue Date: Oct-2014
metadata.dc.creator: AGUIAR, Bruno Guedes Alcoforado
COELHO, Daniela Lemos
COSTA, Dorcas Lamounier
DRUMOND, Betânia Paiva
COELHO, Luiz Felipe Leomil
FIGUEIREDO, Lívio Carvalho
ZACARIAS, Danielle Alves
SILVA, Jailthon Carlos da
ALONSO, Diego Peres
RIBOLLA, Paulo Eduardo Martins
ISHIKAWA, Edna Aoba Yassui
GAÍDO, Samara Belchior
COSTA, Carlos Henrique Nery
Title: Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum
Citation: AGUIAR, Bruno Guedes Alcoforado et al. Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by leishmania infantum. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 47, n. 5, p. 593-598, out. 2014. Disponível em: <>. Acesso em:.
Abstract: Introduction: Kala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar. Methods: To determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification. Results: The nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival. Conclusions: NAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis.
Keywords: Diversidade genética
Leishmaniose visceral
Fator de inibição de macrófagos
Doenças tropicais
Polimorfismo de nucleotídeo único
Series/Report no.: Revista da Sociedade Brasileira de Medicina Tropical
ISSN: 0037-8682 Brasil
Publisher: Universidade Federal do Pará
metadata.dc.publisher.initials: UFPA
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - NMT

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