Use este identificador para citar ou linkar para este item: http://10.7.2.42:8080/jspui/handle/2011/1704
Título: Avaliação da técnica de aspiração ovocitária transvaginal (Ovum Pick Up) e produção in vitro de embriões da raça nelore (Bos taurus indicus) oriundos de doadoras com alterações da fertilidade
Título(s) alternativo(s): Evaluation of the technique of aspiration ovocitária transvaginal (Ovum Pick Up) and production in vitro of embryos of the nelore race (Bos taurus indicus) deriving of givers with alterations of the fertility
Autor(es): OHASHI, Otávio Mitio
http://lattes.cnpq.br/5547874183666459
Palavras-chave: Produção animal
Inseminação artificial
Fertilização in vitro
Zebuínos
Aspiração folicular
Data do documento: 11-Set-2003
Instituição: Universidade Federal do Pará
Empresa Brasileira de Pesquisa Agropecuária
Universidade Federal Rural da Amazônia
Citar como: SOUSA, Alysson Jorge de Oliveira. Avaliação da técnica de aspiração ovocitária transvaginal (Ovum Pick Up) e produção in vitro de embriões da raça nelore (Bos taurus indicus) oriundos de doadoras com alterações da fertilidade. 2008. 62 f. Dissertação (Mestrado) – Universidade Federal do Pará, Núcleo de Estudos em Ciência Animal, Empresa Brasileira de Pesquisa Agropecuária, Universidade Federal Rural da Amazônia, Belém, 2003. Programa de Pós-Graduação em Ciência Animal.
Abstract: The objective of this experiment was evaluate the possibility of the use of zebu cattle females with raised genetic quality presenting problems of fertility, acquired after a program of conventional transference of embryos, as oocyte donors in a program of Ovum Pick Up and In Vitro embryo production. Sixteen females of Nelore breed (Bos taurus indicus) with average age of 11 years and 7 months, had been submitted to the same conditions of enviroment, feeding and management. These animals were removed of the program of embryo transfer due fertility problems (ovarian cysts, genital infections and some cases of repeat breedings). The animals had been divided in two groups in agreement the type of pathology: Group I (n=08) formed by animals that had presented ovarians patologies (cysts) and GROUP II (n=08) formed by animals with extra-ovarians patologies, both groups had been submitted to aspiration follicular (OPU) using two types of needles (21G ; 19G ) and pressures (55 e 70mmHg). The two groups were divided in four treatments (T1 =55/21G; T2 = 55/19G; T3 = 70/21G e T4 = 70/19G) and after oocites collection they were submitted to IVP. In this work, 149 aspirations were realised and 879 oocytes were collected (5,9 4,88) GRADE I = 16%; GRADE II = 43%; GRADE III = 21% e GRADE IV = 20%. The averages of oocytes collected in Group I were (T1 = 7,06 5,10; T2= 7,00 3,64; T3 = 10,60 6,51 and T4 = 2,78 2,59) and the second group (GROUP II) (T1 = 4,90 4,47; T2= 2,93 3,05; T3 = 6,11 4,11 and T4 = 2,70 2,00). For the averages of viable oocytes, the results had been the following ones (T1 = 4,35 3,01; T2 = 4,88 2,96; T3 = 6,87 3,91 and T4 = 1,89 1,90) for GROUP I and (T1 = 2,70 2,22; T2 = 1,92 1,98; T3 = 3,47 2,27 and T4 = 2,00 1,05) for GROUP II. In this experiment, 538 oocytes had been taken to the IVP, 161 (32,85%) of these oocytes had clived and 65 (13,26%) had resulted in Morula or Blastocysts. The statistcs analysis of the results did not demonstrate significant difference in the oocyte number of the diferent groups, caused for the suction pressure. It was observed that the type of needle is related of pressure of the vacuum bomb, the best results were finding with T3 treatment, moreover It can be observed through these findings that the best averages of oocytes collected had been gotten in the group of animals that had presented ovarians cysts (GROUP I), We concluded in this experiment that the use of OPU/IVF technique is an great option in the prolongation of the reproductive life in problems zebu cattle.
URI: http://www.repositorio.ufpa.br:8080/jspui/handle/2011/1704
Aparece nas coleções:Dissertações em Ciência Animal (Mestrado) - PPGCAN/Castanhal

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