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metadata.dc.type: Artigo de Periódico
Issue Date: Sep-1998
metadata.dc.creator: OLIVEIRA, Ricardo Bezerra de
LEAL, Walace Gomes
NASCIMENTO, José Luiz Martins do
DINIZ, Cristovam Wanderley Picanço
Title: Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats
Citation: OLIVEIRA, R.B. et al. Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 31, n. 9, p. 1157-1161, set. 1998. Disponível em: <>. Acesso em: 28 mar. 2012. <>.
Abstract: The effects of methylmercury (MeHg) on histochemical demonstration of the NADPH-diaphorase (NADPH-d) activity in the striate cortex were studied in 4 adult cats. Two animals were used as control. The contaminated animals received 50 ml milk containing 0.42 µg MeHg and 100 g fish containing 0.03 µg MeHg daily for 2 months. The level of MeHg in area 17 of intoxicated animals was 3.2 µg/g wet weight brain tissue. Two cats were perfused 24 h after the last dose (group 1) and the other animals were perfused 6 months later (group 2). After microtomy, sections were processed for NADPHd histochemistry procedures using the malic enzyme method. Dendritic branch counts were performed from camera lucida drawings for control and intoxicated animals (N = 80). Average, standard deviation and Student t-test were calculated for each data group. The concentrations of mercury (Hg) in milk, fish and brain tissue were measured by acid digestion of samples, followed by reduction of total Hg in the digested sample to metallic Hg using stannous chloride followed by atomic fluorescence analysis. Only group 2 revealed a reduction of the neuropil enzyme activity and morphometric analysis showed a reduction in dendritic field area and in the number of distal dendrite branches of the NADPHd neurons in the white matter (P<0.05). These results suggest that NADPHd neurons in the white matter are more vulnerable to the long-term effects of MeHg than NADPHd neurons in the gray matter.
Keywords: NADPH desidrogenase
Córtex visual
ISSN: 1414-431X
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - ICB

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