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metadata.dc.type: Artigo de Periódico
Issue Date: Apr-2013
metadata.dc.creator: MELO, Fernando de Assis Ferreira
BEZERRA, Ana Caroline Fonseca
SANTANA, Bárbara Brasil
ISHAK, Marluísa de Oliveira Guimarães
ISHAK, Ricardo
VALLINOTO, Izaura Maria Vieira Cayres
VALLINOTO, Antonio Carlos Rosário
Title: JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region
Citation: MELO, Fernando Assis Ferreira et al. JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 2, p. 145-149, abr. 2013. Disponível em: <>. Acesso em: 18 jun. 2014.
Abstract: This study evaluated the relative occurrences of BK virus (BKV) and JC virus (JCV) infections in patients with chronic kidney disease (CKD). Urine samples were analysed from CKD patients and from 99 patients without CKD as a control. A total of 100 urine samples were analysed from the experimental (CKD patients) group and 99 from the control group. Following DNA extraction, polymerase chain reaction (PCR) was used to amplify a 173 bp region of the gene encoding the T antigen of the BKV and JCV. JCV and BKV infections were differentiated based on the enzymatic digestion of the amplified products using BamHI endonuclease. The results indicated that none of the patients in either group was infected with the BKV, whereas 11.1% (11/99) of the control group subjects and 4% (4/100) of the kidney patients were infected with the JCV. High levels of urea in the excreted urine, low urinary cellularity, reduced bladder washout and a delay in analysing the samples may have contributed to the low prevalence of infection. The results indicate that there is a need to increase the sensitivity of assays used to detect viruses in patients with CDK, especially given that polyomavirus infections, especially BKV, can lead to a loss of kidney function following transplantation.
Keywords: Insuficiência renal crônica
Infecções por polyomavirus
Transplante de rim
Vírus BK
Vírus JC
Amazônia brasileira
ISSN: 0074-0276
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - ICB

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