Please use this identifier to cite or link to this item: http://repositorio.ufpa.br/jspui/handle/2011/6589
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dc.creatorRAMOS, Carlos Alberto do Nascimento-
dc.creatorARAÚJO, Flábio Ribeiro de-
dc.creatorSANTOS, Rafaelle Cunha dos-
dc.creatorMELO, Elaine Silva de Pádua-
dc.creatorSOUSA, Letícia Carvalho de-
dc.creatorVIDAL, Carlos Eugênio Soto-
dc.creatorGUERRA, Neurisvan Ramos Guerra-
dc.creatorRAMOS, Rafael Antonio do Nascimento-
dc.date.accessioned2015-04-28T14:31:25Z-
dc.date.available2015-04-28T14:31:25Z-
dc.date.issued2014-
dc.identifier.citationRAMOS, Carlos A.N. et al. Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 199-204, 2014. Disponível em: <http://www.scielo.br/pdf/bjm/v45n1/7118.pdf>. Acesso em: 24 abr. 2015. <http://dx.doi.org/10.1590/S1517-83822014005000039>.pt_BR
dc.identifier.issn1517-8382-
dc.identifier.urihttp://repositorio.ufpa.br/jspui/handle/2011/6589-
dc.description.abstractThe recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.pt_BR
dc.language.isoengpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBovinopt_BR
dc.subjectAnaplasma marginalept_BR
dc.subjectTestes de fixação do latexpt_BR
dc.subjectMSP5pt_BR
dc.titleDevelopment and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattlept_BR
dc.typeArtigo de Periódicopt_BR
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