Please use this identifier to cite or link to this item: http://repositorio.ufpa.br/jspui/handle/2011/6870
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Type: Artigo de Periódico
Issue Date: Jun-2014
Authors: DEGRANDI, Tiago Marafiga
PITA, Sebastian
PANZERA, Yanina
OLIVEIRA, Edivaldo Herculano Correa de
MARQUES, José Ribamar Felipe
FIGUEIRÓ, Marivaldo Rodrigues
MARQUES, Larissa Coelho
VINADE, Lucia Helena do Canto
GUNSKI, Ricardo José
DEL VALLE GARNERO, Analía
Title: Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed
Citation: DEGRANDI, Tiago Marafiga et al. Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed. Genetics and Molecular Biology, Ribeirão Preto, v. 37, n. 2, p. 375-380, jun. 2014. Disponível em: <http://www.scielo.br/pdf/gmb/v37n2/a09v37n2.pdf>. Acesso em: 25 ago. 2015. <http://dx.doi.org/10.1590/S1415-47572014000300009>.
Abstract: Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the “river buffaloes” with 2n = 50 and the “swamp buffaloes”, 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs (3p, 4p, 6, 21, 23, 24) in the river buffaloes, whereas the swamp buffaloes presented only five NOR-bearing pairs (4p, 6, 20, 22, 23). The F1 crossbreed had 11 chromosomes with active NORs, indicating expression of both parental chromosomes. FISH analysis confirmed the numerical divergence identified with Ag-NOR. This result is explained by the loss of the NOR located on chromosome 4p in the river buffalo, which is involved in the tandem fusion with chromosome 9 in this subspecies. A comparison with the ancestral cattle karyotype suggests that the NOR found on the 3p of the river buffalo may have originated from a duplication of ribosomal genes, resulting in the formation of new NOR sites in this subspecies.
Keywords: Bubalinos
Bubalus bubalis
Citogenética
DNA
ISSN: 1415-4757
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - FACIN/ICEN

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