Please use this identifier to cite or link to this item: http://10.7.2.42:8080/jspui/handle/2011/9118
Title: Perfil epidemiológico, letalidade e caracterização molecular de Pseudomonas aeruginosa multirresistentes, produtoras de metalobetalactamases de isolados clínicos em Unidade de Terapia Intensiva na região Norte
Authors: LIMA, Karla Valéria Batista
http://lattes.cnpq.br/9795461154139260
CARNEIRO, Irna Carla do Rosário Souza
http://lattes.cnpq.br/4389330944043163
Keywords: Epidemiologia
Pseudomonas aeruginosa
Infecção por PaMR
Resistência bacteriana
Infecção hospitalar
Belém - PA
Pará - Estado
Issue Date: 2014
Publisher: Universidade Federal do Pará
Citation: MATOS, Eliseth Costa Oliveira de. Perfil epidemiológico, letalidade e caracterização molecular de Pseudomonas aeruginosa multirresistentes, produtoras de metalobetalactamases de isolados clínicos em Unidade de Terapia Intensiva na região Norte. 2014. 163 f. Tese (Doutorado) – Universidade Federal do Pará, Núcleo de Medicina Tropical, Belém, 2014. Programa de Pós-Graduação em Doenças Tropicais.
Abstract: The bacterial resistance is responsible for major concerns about the great problems on health systems. The proposal of this study was to determine the epidemiological, lethality and molecular characteristics of Pseudomonas aeruginosa isolated from patients admitted to the Intensive Care Unit (ICU) in a teaching hospital in city of Belem, Pará, Brazil and analyze the performance of susceptibility testing for P. aeruginosa in automated VITEK -2 to study the antimicrobial resistance. The target people of this study consisted of 54 patients with P. aeruginosa infection at the adult , pediatric and neonatal ICUs from January 2010 to March 2012. For the epidemiological research, demographic data, comorbidities, length of stay were collected. Episodes of Healthcare-associated infections were defined in accordance to Center for Disease Control and Prevention (CDC), use of invasive procedures, previous therapy and patient outcome, Jackson MacCabe score, APACHE II score and Charlson score were documented. The bacterial identification was performed by biochemical tests and VITEK-2 system. The sensitivity test was performed with the application of the method of broth microdilution , and detection of metallo-beta-lactamase by the double disc and PCR method. The test of Genotyping was made by amplification throug DiversiLab system. The P. aeruginosa was the second most common pathogen in the hospital and this occurrence was higher in the adult ICU. The respiratory infection was predominant in the adult ICU, while bloodstream infection was prevalent in pediatric and neonatal ICU. The multidrug resistance was higher in the adult ICU, the average age of patients was 28 years, with an average length of stay of 87.1 days, the diseases were potentially fatal and major risk factors for acquisition of PaMR infection were the use of mechanical ventilation and urinary catheterization. Through the meta-analysis study, we found that infections caused by MR strains showed higher mortality rate, and were twice as likely to death occurs, multidrug resistance a factor of poor prognosis in patients with infection PaMR. Four isolates producers blaSPM-1 variant was detected and genotyping figured the presence of genetic similarity greater than 97%, the changes observed may be related to selective pressure existing in the adult ICU, coupled with inadequate therapy. This study demonstrated the importance of investigations of infections with multidrug-resistant pathogens in critical care units, contributing to these data the possibility of better monitoring and control of these infections.
URI: http://repositorio.ufpa.br/jspui/handle/2011/9118
Appears in Collections:Teses em Doenças Tropicais (Doutorado) - PPGDT/NMT

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