Programa de Pós-Graduação em Doenças Tropicais - PPGDT/NMT
URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/3558
O Programa de Pós-Graduação em Doenças Tropicais (PPGDT) integra o Núcleo de Medicina Tropical (NMT) da Universidade Federal do Pará (UFPA), realizando atividades de ensino, pesquisa e extensão e atuando na formação de docentes-pesquisadores para o estudo e o ensino das doenças tropicais e das patologias regionais no estado do Pará e na Amazônia.
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Navegando Programa de Pós-Graduação em Doenças Tropicais - PPGDT/NMT por Orientadores "BATISTA, Evander de Jesus Oliveira"
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Item Acesso aberto (Open Access) Avaliação in vitro dos efeitos de miltefosina, orizalina e TC95 no crescimento de Entamoeba histolytica(Universidade Federal do Pará, 2011) ALVARENGA, Betânia Mara; BATISTA, Evander de Jesus Oliveira; http://lattes.cnpq.br/2206444845201080Amebiasis is characterized as a chronic, noninflammatory, afebrile which involves the colon wall and can invade the intestinal mucosa and spread to other organs, especially the liver. Entamoeba histolytica infection is treated with metronidazole, but this one presents side effects, so researches have been done to find more effective drugs with no adverse effects to the patient. In addition, testing new drugs may reveal important features of the parasite, such as the discovery of metabolic pathways or possible differences in their structure. In the present study, it was tested the amoebicidal effect of metronidazole, dinitroaniline oryzalin, a microtubule inhibitor, miltefosine, an alkylphosphocholine, that inhibits the membrane phospholipids syntesis, and TC95, a hybrid drug that presents the functional groups dinitroaniline and alkylphosphocholine. It was performed a culture with protozoan trophozoites, where the drugs and the control with diluents were added after 7 hours of culture. It was made a count of the viable trophozoites 12 hours after the addition of the drugs and the control at intervals of 12 to 60 hours. After analyzing the growth curves, it was found that the TC95 was the most effective drug of the three treatments, miltefosine had little inhibition and oryzalin was not effective. This is due to greater susceptibility of the parasite to membrane damage, with little effectiveness on microtubules inhibition.Item Acesso aberto (Open Access) Caracterização sérica da lectina ligadora de manose (MBL) em indivíduos portadores de parasitoses intestinais(Universidade Federal do Pará, 2012) ALENCAR, Maria de Nazaré Costa Santos; VALLINOTO, Antonio Carlos Rosário; http://lattes.cnpq.br/3099765198910740; BATISTA, Evander de Jesus Oliveira; http://lattes.cnpq.br/2206444845201080Although the intestinal parasitosis are known since a long time and be studied since its identification, still constitute a challenge for their diagnosis and treatment. The delineation of the problem coming up in the necessity of investments in research for more accurate diagnosis, for the prompt intervention, to the existing cases and work for the prevention of risk factors that favor the emergence, maintenance and propagation of these agents. The knowledge that the host's immune competence is a limiting factor of parasitic load of various species, stimulated the research considering that the Mannosebinding lectin (MBL), a component of the complement system, is a key protein of the innate immune system, acting in the first line of defense against pathogens because it is considered acute phase. In this study, were colected 221 samples of individuals of both gender and varied ages at three different laboratories, in the period from January to April 2012. The profile of the population of the study are made and the analysis of the association between social and demographic factors with the entero-parasites and evaluated the influence of serum levels of Mannose-binding lectin (MBL) susceptibility of enteroparasitoses, distribution by age group and gender. Established also the comparison between serum concentrations of MBL of groups with identification of parasites. Statistically significant associations were observed when related protozoa E. histolytica and G.lamblia with the serum concentration of the MBL.Item Acesso aberto (Open Access) Equinococose neotropicial: um estudo anatomopatológico para auxílio em uma sistematização diagnóstica correlacionada a morfologia(Universidade Federal do Pará, 2012) ABREU JUNIOR, José Maria de Castro; SOARES, Manoel do Carmo Pereira; http://lattes.cnpq.br/1483308844442427; BATISTA, Evander de Jesus Oliveira; http://lattes.cnpq.br/2206444845201080Equinococcosis is a disease caused by cestodes of the Echinococcus genus, that can be found at the small intestin of animals like bush-dog and domestic dog. Men behave as an accidental host by the ingestion of eggs of the parasite. The larval forms mainly develop at the liver, and sometimes at the lung, causing a cronic diesease that can be easily mistaken by neoplasias. At the State of Pará, North Brazil, there is a great number of cases of this rare diesease, related to Echinococcus vogeli in the Amazon.This study describes six new cases, five in the the liver, and one at the lung, making description a about the kind of inflammatory cells. Fibrosis, giant-cells, calcification and the laminated layer with the typical cerebroid pattern. Rostellar hooklets did not were found. The laminated layer, is not enough to describe the species of Echinococcus, but allows to consider that the worm´s gender is Echinococcus. Even though the number of cases is too small for a statistical analysis, were observed cells that can justify why the disease has a cronic evolution. More works like this, adding more cases are important, because equinococcosis is a neglected diesese.Item Acesso aberto (Open Access) Frequência e diversidade de enteroparasitos veiculados por hortaliças comercializadas na cidade de Belém-PA e sua relação com a sazonalidade climática(Universidade Federal do Pará, 2010) BORGES, Samuel da Luz; BATISTA, Evander de Jesus Oliveira; http://lattes.cnpq.br/2206444845201080The intestinal parasites are an important healthy problem to human population worldwide. The consumption of vegetables is one of the major routes of transmission of this pathogens. This study aimed to determine the frequency and diversity of intestinal parasites transmitted by vegetables sold in the metropolitan region of Belém-PA and its relation to the seasonal climate of the region. It was used 252 samples of three species of vegetables, of which 84 were of lettuce (Lactuca sativacurly variety), 84 were watercress (Nasturtium officinale) and 84 were coriander (Coriandrum sativum) purchased in open markets, gardens and in a supermarket, from November of 2008 to December of 2009. Each samples was washed with 500ml of PBS, permitting the spontaneous sedimentation and centrifuging 30 ml of the final sediment. The final sediment was analyzed in an optical microscope. The levels of contamination of the three species of vegetables were obtained by the monthly average of intestinal parasite structures identified in each vegetable and by the total number of parasites identified in the samples of each open market, garden and supermarket. In the results obtained in the microscopic analysis of samples were applied the Chi-square and Fisher's Exact Test to determine the existence or not of significant differences between these results. It was used the significance level α ≤ 0.05. This microscopic analysis showed a contamination of 100% of the samples at open markets, gardens and supermarket included in the study, there isn’t statistical difference in overall frequency of parasites among them. Strongyloides stercoralis was the most prevalent parasite, followed by complex Entamoeba histolytic / dispar and the hookworms, both in samples from the gardens, as in samples of the open markets and the supermarket. The cress and lettuce had higher rates of parasitic contamination that coriander. It had been characterized seasonal influence on the intensity of parasites in vegetables investigated because there was statistical difference between the results obtained with a higher prevalence of parasites in samples of summer, for the samples of winter. There wasn’t also statistically significant difference between the monthly averages of contamination of vegetables sold in open markets, gardens and supermarket, indicating that the hygienic conditions under which the vegetables are sold, although important to keep their flavors, have less influence on levels of parasitic contamination, which seems to be associated with the place and growing conditions these plants. These datas permit a good level of comparison to future studies.Item Acesso aberto (Open Access) Prevalência de HPV em material cérvico-uterino de mulheres de Tomé-Açú – PA(Universidade Federal do Pará, 2011) PRAZERES, Benedito Antônio Pinheiro dos; SOUSA, Maísa Silva de; http://lattes.cnpq.br/1775363180781218; BATISTA, Evander de Jesus Oliveira; http://lattes.cnpq.br/2206444845201080The human papillomavirus (HPV) infects basal of stratified epithelium and develop proliferative benign lesions in skin or mucous. The infection has a universal distribution however many studies have demonstrated a strong association of infections by high risk species in cases of cervical cancer. In Brazil, this type of cancer is the second most common among women, while the north and northeast region of this country have the highest incidence. Therefore, this study has as focus the determination of infection prevalence in a group of women screened to cervical cancer. During the period of July 2008 to March 2009 were collected cervical samples from 144 women received at Laboratório de Citopatologia of Hospital Amazônia from Quatro Bocas, Tomé-Açú, Pará. All women were informed about the purpose of that research and then signed the Consent Form and answered the epidemiological questionnaire. The data obtained from the questionnaire correlated with the infection through the testing qui -quadradot. The HPV prevalence was 6,94% and the majority age was between 18-28 years. Samples from 76 patients showed inflammatory aspect (52,02%) and 60 patients did not showed any change (41,09%). Among smears with cytological ASC-US in the most of cases (6/10) followed by LSIL (2/10), and ASC-H (1/10) and HSIL (1/10). The HPV infection is statistically significant correlated PCR-HPV test with the age group, and cytomorphology. Prevalence found in this study corroborants with other findings reported in the literature. The predominance of infection in women with abnormal cytology strengthens the concept that infection is, in most of cases, asymptomatic, and the Pap smear method is lesser efficient to detect infection than techniques of molecular biolology.Item Acesso aberto (Open Access) Prevalência de HTLV-1 e HTLV-2 em portadores de Strongyloides stercoralis, Pará, Brasil(Universidade Federal do Pará, 2012-03-12) AGUIAR, Samantha Assis de; SOUSA, Maísa Silva de; http://lattes.cnpq.br/1775363180781218; BATISTA, Evander de Jesus Oliveira; http://lattes.cnpq.br/2206444845201080The Strongyloides stercoralis is an intestinal nematode of humans that causes strongyloidiasis, a disease with worldwide distribution particularly common in tropical and subtropical regions. Epidemiological studies have demonstrated the existence of an association of this parasitic disease with Human T-cell Lymphotropic Virus type 1 (HTLV-1). In regions where both agents are endemic, the coinfection may result in the development of severe strongyloidiasis, because HTLV-1 causes a reduction in the production of immune components participating in the defense mechanisms against S. stercoralis. Based on this question, this study wanted to help and clarify the role of immunosuppression induced by HTLV-1 and HTLV-2 in the persistence and dissemination of Strongyloides stercoralis. Serological and molecular tests were used to assess the frequency of HTLV-1 and HTLV-2 in patients with S. stercoralis treated at University Hospital João de Barros Barreto, Belém Pará, in the period July 2009 to June 2011. In this study, we observed the frequency (5.50%) of anti-HTLV-1 and HTLV-2 in patients with Stongyloides stercoralis. The prevalence of HTLV-1 (3.67%) was higher than that of HTLV-2 (0.92%). The analysis of the sample showed no statistically significant differences in the frequency of HTLV-1 and HTLV-2 among men and women. At the distribution of patients with HTLV-1 and HTLV-2 by age group, we observed a higher frequency of the virus among patients with older age. Based on the results in this study, we conclude that there is need for prophylactic measures to prevent the spread of HTLV-1 and HTLV-2 among carriers of S. stercoralis and consequently prevent the development of complications resulting from the combination of these agents.