Dissertações em Biologia de Agentes Infecciosos e Parasitários (Mestrado) - PPGBAIP/ICB
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/4738
O Mestrado em Biologia de Agentes Infecciosos e Parasitários teve início em 2004 e funciona no Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários (PPGBAIP) do Instituto de Ciências Biológicas (ICB) da Universidade Federal do Pará (UFPA).
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Navegando Dissertações em Biologia de Agentes Infecciosos e Parasitários (Mestrado) - PPGBAIP/ICB por Orientadores "VALLINOTO, Antonio Carlos Rosário"
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Item Acesso aberto (Open Access) Estudo da susceptibilidade à infecção pelo HIV-1 e da progressão da AIDS em associação ao polimorfismo no gene Mbl (Lectina Ligadora de Manose)(Universidade Federal do Pará, 2004-09-12) COSTA, Marcos Rogério Menezes da; VALLINOTO, Antonio Carlos Rosário; http://lattes.cnpq.br/3099765198910740The low serum concentration of Mannose-Binding Lectin (MBL) is associated to the presence of variant alleles Mbl-*B, Mbl-*C and Mbl-*D, and it results in an increased susceptibility to recurrent infections. The present study investigated the association between the Mbl gene polymorphism and the susceptibility to HIV-1 infection. A fragment of 349 bp from the exon 1 of the Mbl gene was amplified by PCR and then submitted to RFLP analysis using the endonucleases BanI and MboII, aiming the identification of the variant alleles. The study of 145 seropositive patients and 99 healthy controls showed the presence of alleles Mbl-*A, Mbl-*B and Mbl-*D, with frequencies of 69%, 22% and 9% among patients and 70.2%, 13.6% and 16.2% among healthy controls, respectively. The analysis of the genotype frequencies showed a high prevalence of the genotypes carriers of variant Mbl-*B among patients seropositive as compared to the healthy controls. Furthermore, the genotype B/B was six times more frequent among patients than the observed to the healthy controls (χ2=4.042; p=0.044). The mean viral load was lower in HIV-1 seropositive patient carrying the Mbl-*A allele than those carrying the variant Mbl-*B allele (5,821 copies/mL vs. 52,253 copies/mL; p= 0.05). Furthermore, patients carrying the allele Mbl-*A showed a significant reduction of the viral load (p<0.001), that was not observed among those carrying the variant Mbl-*B (p=0.999). The results suggest the importance of the Mbl gene polymorphism on the clinical evolution of the patients infected by HIV-1 and that the identification of the Mbl genetic profile, among HIV-1 infected patients, may be an important tool to monitor the evolution and the prognosis of diseases.Item Acesso aberto (Open Access) Investigação do polimorfismo do exon - 1 do gene MBL (Mannose-Binding Lectin) em pacientes portadores de tuberculose(Universidade Federal do Pará, 2009-03-31) ARAÚJO, Mauro Sérgio Moura de; VALLINOTO, Antonio Carlos Rosário; http://lattes.cnpq.br/3099765198910740Mannose-binding lectin (MBL) is considered an acute phase protein with important role in the first line of defense of the innate immune system, whose serum levels are genetically determined. The MBL activates the lectin pathway of complement, and mediate the phagocytosis of microorganisms and opsonization. Several studies have associated serum levels of MBL to susceptibility or resistance to infectious agents including the Mycobacterium tuberculosis, causative agent of human tuberculosis. Aiming to evaluate the occurrence of a possible association between MBL gene polymorphisms and tuberculosis, it was evaluated the frequencies of mutations in exon 1 of MBL gene in a group of 167 TB patients, divided into 3 groups: patients with pulmonary tuberculosis, with extrapulmonary tuberculosis, patients with multiresistant tuberculosis to drugs and the control group with 159 health professionals. The identification of alleles MBL*A, *B, *C and *D was performed by polymerase chain reaction, using specific sequences of primers and subsequent enzymatic digestion. The analysis of allelic and genotypic frequencies of exon 1 did not show any significant difference between patients with tuberculosis and control group (p> 0.05). There were no significant associations between groups of pulmonary tuberculosis, extrapulmonary tuberculosis and multidrug drug among themselves and when connected to the control group. Data from our study showed no evidence of any influence of variations in the exon 1 of MBL gene in active tuberculosis, suggesting that the gene polymorphism has no influence on susceptibility to tuberculosis.