Programa de Pós-Graduação em Oncologia e Ciências Médicas - PPGOCM/NPO
URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/4631
O Programa de Pós-Graduação em Oncologia e Ciências Médicas (PPGOCM) integra o Núcleo de Pesquisas em Oncologia (NPO) da Universidade Federal do Pará (UFPA). Trata-se do único centro de referência em pesquisa e formação de recursos humanos stricto sensu na área de oncologia na região Norte do Brasil. Os outros centros se concentram nas cidades do Rio de Janeiro e São Paulo.
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Item Acesso aberto (Open Access) Análise da expressão gênica diferencial entre o adenocarcinoma da junção esôfago-gástrica e o adenocarcinoma gástrico(Universidade Federal do Pará, 2016-03-03) MASCARENHAS JUNIOR, Rui Wanderley; ASSUMPÇÃO, Paulo Pimentel de; http://lattes.cnpq.br/7323606327039876Gastric cancer is the fifth most common malignancy worldwide and the third in mortality. In 2010, UICC released the latest edition of the TNM staging manual in the adenocarcinoma esophageal-gastric junction (JEG) with epicenter at 5 cm from the JEG and extending into the esophagus is classified and staged together with the esophageal tumors, while those that do not extend into the esophagus remain classified and staged as gastric adenocarcinoma. This change was due to differences between adenocarcinomas of the JEG and stomach, risk factors, treatment and prognosis. With the development of molecular biology, several areas - such as transcriptomics, studying gene expression on a genomic scale - started to be used to explore differences in expression in various tumor types. In this sense, the Atlas Genomic Cancer (TCGA) is a database that aims to generate complete maps of the genomic key changes of the major types of cancer, in order to accelerate the understanding of the molecular basis of cancer through the application of technology genome analysis, which makes it a powerful tool in this area. Considering these aspects, the aim of this study is to comparatively analyze the transcriptome of adenocarcinomas of the JEG and gastric using TCGA data as a tool for validation. To this end, the gastrectomy at the University Hospital João de Barros Barreto eight patients samples were obtained submitted, four adenocarcinomas of the JEG and four gastric adenocarcinomas. Samples were analyzed using the technique of expression microarray chips with Human Gene 1.0 ST Array (Affymetrix®) which allow the analysis of 36,079 transcripts. The transcriptome analysis revealed 36 genes differentially expressed (fold-change greater than or equal to 5), 11 and 25 hipoexpressos hiperexpressos genes in JEG adenocarcinoma in relation to gastric adenocarcinomas. In the analysis of TCGA were identified 509 differentially expressed genes (p <0.05), and the ASPN genes, LiPF HNRNPM and validated for this database. Thus, it is concluded that the differential gene analysis shows significant changes between gastric adenocarccinoma and JEG and its molecular differences may reflect the clinical features, stressing that these tumors should be classified and staged differently.Item Acesso aberto (Open Access) Capacitação de Agentes Comunitários de Saúde para a prevenção e controle do câncer(Universidade Federal do Pará, 2014-05-08) BRITO, Leidiane Mendes; ASSUMPÇÃO, Paulo Pimentel de; http://lattes.cnpq.br/7323606327039876The present study has as main topic of discussion the Community Health Agent as essential actor for prevention and control of cancer, along with the staff of FHS. We focus our attention here specifically, through the eyes of the CHW, the risks and to facilitate the development of cancer attitudes, as well as alarm signals. We had as guiding to this research the following question: the Community Health Workers, after receiving proper training, attain sufficient skills to work in cancer control, through actions of risk identification and health education? So if systematized the main objective, which was to develop a model of intervention that contribute to cancer control in primary care, with the CHW as the primary mediator. This is an intervention research, cross-sectional qualitative approach, having as object of study, the work of CHW as an instrument of the ESF for cancer control. A sample of five CHW's was selected as the main participants and eight families as secondary participants. For production data, a field research was used, which included the observational method. This production was divided into two stages: a study of effectiveness and efficiency study. Regarding data analysis, we had two periods of interpretation seeking to organize our discussion into two interpretive matrices: considerations of the activities and the corollary of the actions, each with their respective categories and themes. This analysis relied on the theoretical and methodological support of thematic content analysis proposed by Bardin (2011). As for the results, we implement after prior intervention plan, we could reach results supported the model of intervention that we propose to research. Thus, it was possible to see how this should be organized and what steps should be performed. At first, this intervention has sparked changes in the daily lives of participants, resulting in positive health behaviors. The CHW's met expectations and after work training performed well in research and health education activities. Therefore, this research still can not answer whether the intervention produced, in short, is capable of causing long-term changes, and especially if these changes have helped in reducing cancer rates. It is worth to notice that such interventions to be successful, the partnerships need to be strengthened. This way, we can have an efficient public health and at the same time, a primary care quality, it requires in fact, that all work together.Item Acesso aberto (Open Access) Expressão dos genes TFF1 e TFF2 em adenocarcinoma gástrico(Universidade Federal do Pará, 2014-01-24) HAGE, Pedro Antônio Mufarrej; CALCAGNO, Danielle Queiroz; http://lattes.cnpq.br/1326603355062154; ASSUMPÇÃO, Paulo Pimentel de; http://lattes.cnpq.br/7323606327039876Gastric cancer remains a serious public health problem with high morbidity and mortality. Generally, the diagnoses occur in advanced disease when the available therapeutic options have limited effectiveness. Despite advances in the understanding of carcinogenesis of gastric adenocarcinoma, particularly on genetic and epigenetic mechanisms involved, the clinical aplicabilitadade such knowledge remains limited. In order to identify potential biomarkers in gastric cancer, we conducted a study using microarray comparing gene expression in gastric adenocarcinomas and paired samples of non- neoplastic gastric mucosa. Preliminary, the results showed significant differences in expression of 53 genes. Among these, the TFF1 and TFF2 genes were selected for validation of expression by real-time PCR in 78 additional samples. Expression of TFF1 and TFF2 were significantly reduced in samples of gastric adenocarcinoma when you compare the paired non-neoplastic tissues (p<0.05). Additionally, the TFF2 gene expression was significantly lower in the intestinal subtype than in the diffuse subtype. The expression of the two genes showed a strong correlation, the similar pattern of expression suggests that TFF1 and TFF2 may have common regulatory elements. This hypothesis is enhanced due to the small physical distances between them. The results suggest the involvement of TFF1 and TFF2 in gastric carcinogenesis and demonstrate the potential for clinical use of these genes as biomarkers and potential therapeutic targets in gastric adenocarcinoma.Item Acesso aberto (Open Access) Investigação de polimorfismos nos genes IFNɣ e INFGR1 associados à tuberculose no estado do Pará(Universidade Federal do Pará, 2015-11-06) CARNEIRO, Klezzer de Oliveira; SANTOS, Ney Pereira Carneiro dos; http://lattes.cnpq.br/1290427033107137; ASSUMPÇÃO, Paulo Pimentel de; http://lattes.cnpq.br/7323606327039876Pulmonary tuberculosis is an infectious disease transmission by air, which according to the World Health Organization (WHO), infects about two billion people around the world. It is the leading cause of death from infectious disease in adults in developing countries, representing a serious public health problem, mainly due to non-adherence to treatment, late diagnosis and underdiagnosis and no control contacts, which makes our population susceptible to infection. The present study aimed to investigate associations three genetic polymorphisms in IFNɣ and INFGR1 genes responsible for susceptibility to tuberculosis in patients affected by the disease; evaluate if there are differences in allelic and genotypic frequencies of polymorphisms in genes IFNɣ 871A> T, INFGR1 611 (C> T) and INFGR1 -56 (A> G) among individuals with TB and those without TB population of Bethlehem. The control substructures effect was carried out by the use ofa 48 markers Informational Genetic Ancestry panel in both patient sample and the control sample. For this study we used peripheral blood samples from 148 patients diagnosed with tuberculosis, and 125 individuals without tuberculosis (controls) resident in the State of Pará, Brazil, attended at University Hospital João de Barros Barreto (HUJBB) during the period from 2006 to 2012. each specimen was obtained 5 ml of venous blood collected from a peripheral vein. DNA extraction was performed according to the method described by Sambrook et al., (1989). Genotyping for polymorphisms IFNɣ gene (rs1130562) and INFGR1 (rs1327474, rs2234711) was performed by PCR in real time (RTQ-PCR) using the TaqMan system. Statistical analyzes were performed in SPSS 17.0 software, using the Mann- Whitney test, with significance set at p <0.05. The results did not show significance of the polymorphisms investigated in relation to susceptibility to tuberculosis.