Navegando por Orientadores "GOUVEIA JUNIOR, Amauri"

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Acesso aberto (Open Access)
Adaptação da Escala de Ansiedade de Beck para avaliação de surdos e cegos
(Universidade Federal do Pará, 2013-09-05) SANCHEZ, Cintia Nazare Madeira; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
This study met three articles concerning the adaptation of psychological instruments to evaluate the population of deaf and blind. Considering that these instruments are not adapted to evaluate people with special educational needs, complicating diagnosis and prognosis. Therefore the need to adapt these instruments for this population is indisputable. This situation also occurs in the area of deafness and blindness, in which there is a shortage of jobs in Brazil. In the first article was conducted a literature review of instruments adapted for this population. It is concluded that the area of hearing assessment scales are adapted to various factors as psychometric for measuring depression, anxiety and intelligence, but the area of blindness instruments are suited for the assessment of cognitive functioning. The aim of the second study was to adapt the Beck Anxiety Scale (BAI) to sign language and alphabet digital generating a scale to assess anxiety in deaf users of Brazilian Sign Language (LIBRAS). The sample consisted of 25 deaf users LIBRAS (experimental group) and 25 listeners (control group), aged between 18 and 25 years of age of both sexes, matched for age and sex. The application was made in a group. Following the guidelines, the subjects completed the scale, the scale pattern control group and the experimental group the adapted scale. The results of the study showed that the BAI adapted not statistically significant compared to the standard scale and total anxiety subscales: subjective, neurophysiological, autonomic, and panic. Therefore BAI adapted showed validity equivalent to BAI standard to assess anxiety in deaf, the items appear to have adapted the modified factor structure of the instrument, thus allowing its use in the assessment of anxiety in deaf users of LIBRAS. The third study was conducted with visually impaired, this deficiency is most prevalent in the population reaching 35.8 million people with difficulty seeing even with corrective lenses, and 506,3 thousand are blind. Despite the significant number of blind in the literature there are few studies of adapting assessment tools for the blind, as they are evaluated on the parameters of the seers. Given this reality, the objective of this study was to adapt the Beck Anxiety Scale (BAI) for Braille generating a scale to assess anxiety in blind Braille users. The sample consisted of 25 blind Braille users (experimental group) and 25 seers (control group), aged between 18 and 25 years of age of both sexes, matched for age and sex. The application was made in a group. Following the guidelines, the subjects completed the scale, the scale pattern control group and the experimental group the adapted scale. The results of the study showed that the BAI adapted not statistically significant compared to the standard scale and total anxiety subscales: subjective, neurophysiological, autonomic. Subscale panic in this difference was statistically significant at the limit. Therefore BAI adapted showed equivalent BAI validity standard for evaluating anxiety blind, adapted items seem to have modified the factor structures of the instrument, allowing their use in the evaluation of anxiety in users blind Braille.
Acesso aberto (Open Access)
Comportamento, ritmo de atividade e arquitetura das galerias de Uca maracoani (Latreille, 1802) e Minuca rapax (Smith, 1870)
(Universidade Federal do Pará, 2016-11-22) BELÚCIO, Lucinice Ferreira; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
This study aimed to elaborate the behavioral budget and to obtain the level of annual activity on the surface as well as the shape of the galleries of Uca maracoani and Minuca rapax, which coexist in the intertidal region of the Curuperé mangrove, Curuçá, Pará, During four sequential lunar periods, from October to December of 2013, the behavior of the species was shot to obtain the budget. The annual activity of the species was evaluated by shooting the number of active animals and galleries at four sites along the river. To evaluate the shape and distribution of the galleries paraffin casts were obtained in two stations with mud sediment and sand-muddy. In the ethogram of the species, ninety-five behaviors were recorded, grouped into the categories: Feeding, Reproductive, Walking and Running, Acoustics and Sysmics, Territorial, Agonistic, Maintenance and Grooming. The following categories were important to differentiate the energy budget of the species: Feeding, Territorial, Acoustics, Reproductive. Considering the difference of niches of males and females of the same species, the following behaviors are noteworthy: Feeding, Walking and Running, Territorial, Maintenance, Acoustics and Sysmics, Reproductive, Grooming. A great amount of rainfall (February) and low humidity (October) decreased animals activity at the surface, while the climate in June allowed a higher activity of these animals at the surface. Despite some similarities in the architecture of the galleries of the two species, the differences in volume and depth suggest that they present individual scales that are very different from influences in the bioturbation process. However, this process can be counterbalanced by the population density of each species and its activity rate.
Acesso aberto (Open Access)
Desamparo aprendido com o zebrafish (Danio rerio)
(Universidade Federal do Pará, 2014-05-23) NASCIMENTO, Gabriela Souza do; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
Exposure to uncontrollable aversive events leads to difficulty in learning contingency relations, escape and/or avoidance. Such phenomenon was named learned helplessness and it has been correlated to depression and post-traumatic stress disorder. This thesis is composed by three studies that together have the aim of: producing learned helplessness in Danio rerio (Study I); investigating the effects of light on the escape test (Study II) and evaluating the effects imipramine have on learned helplessness when applied acutely in Danio rerio (Study III). In every experiment it was used an acrylic shutllebox (INSIGHT equipment) which contained a central area (removable) that enabled the isolation of the subjects. The general protocol consisted in two phases: 1) Treatment, each subject of the Uncontrollable shock (USH) treatment was subjected to 60 random and uncontrollable shocks (0.7 – 0.9V, lasting 30 seconds each), in this phase the subjects of the group that didn't receive treatment with electric shock (NSH) only remained in the central area for 60 minutes; and 2) Test, each subject from both groups was subjected to an escape session with 30 shocks (0.7 – 0.9V, maximum duration of 30 seconds each). In Study I, 3 groups were used, one experimental (EGUSH) and two controls, one remaining in the experimental aquarium for 60 minutes without shock (CG-NSH). In Study II, 4 groups were used, a pair from NSH and USH subjected to the light test, another pair (NSH and USH) underwent the test no light condition. In Study III 6 groups were used according to the concentration of imipramine administered during 10 minutes before the test session: 0.0 mg/l NCH; 0.0 mg/CHI; 1.0 mg/l NCH; 1.0 mg/l CHI; 2.0 mg/l NCH; e 2.0 mg/l CHI. The main results show that it is possible to create learned helplessness in Danio rerio (study I), considering light as a variable that can interfere directly in the acquisition of the phenomenon (study II), in addition, it was showed that acute imipramine in the doses administered here did not revert the learned helplessness in Danio rerio.
Acesso aberto (Open Access)
Desempenho do Guppy (Poecilia reticulata) em modelos de ansiedade: campo aberto, preferência claro-escuro e labirinto em cruz com rampa
(Universidade Federal do Pará, 2016-09-23) MONTEIRO, André Luiz Viard Walsh; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
The use of animal models in experimental research in the last decades has shown to be more diversified than the classical model through the use of rodent or primate. This is mainly by advances in molecular, morphological and functional studies that revealed a big homology between the vertebrates. On this perspective, the Zebrafish (Danio rerio) has shown to be the non-mammal animal with biggest ascension as a study model in biological sciences in the latest decades. However, other species of fish also show to be promising as alternatives of use as animal model. This study used Guppy (Poecilia reticulata) as model for the research in behavior through different experimental approaches. In study I, guppies were exposed and re-exposed in different shifts (morning, afternoon, evening and night) in the open field test and light-dark preference. The found results show that in both tests, male and female present behavioral differences, being sensitive to re-exposure, with capacity of learning e controlling of the circadian cycle. In study II, was developed a plus-maze with ramp where it was verified the sensibility of the specie to the apparatus, the profile of response upon re-exposure and the drug effect. The results revealed sensibility to the apparatus with a height of water column at 8 cm and 5 minutes of session and differences between sex and learning by habituation along the re-expositions. The pharmacological study indicates that in this apparatus, the specie is sensitive to anxiolytic and anxiogenic drugs. Finally, we can conclude that Guppy presents similar behavior and drug responses compatible with the data described to the Zebrafish. These similarities reinforce the use of fishes as an alternative to the use of mammals in animal experimentation.
Acesso aberto (Open Access)
Determinantes do ganhar ou perder em humanos: um estudo com atletas de judô
(Universidade Federal do Pará, 2017-02-17) CAMPOS, Ítalo Sérgio Lopes; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
In the sports environment experiences of success or failure are often experienced by different individuals in the most varied environments. Attempting to explain how an individual deals with victory or defeat in behavioral terms will depend on a number of factors, including athlete's history, status (environment), fitness (ability to perform), and athletic maturity). The general objective of the thesis was to systematize a theoretical model about the determinants of winning and losing in judo. For this purpose, we sought to verify (through documentary analysis) if results of previous fights are decisive for the maintenance of subsequent victories and / or defeats; in sequence the study analyzed judo from a competition situation seeking to describe in real time, possible quantitative and qualitative interactions of the fight. According to the design of this study, it was evidenced that the ability (performance related to fight), allied to athlete's athletic maturity (experience in the modality) were the factors that most potentiated the process of winning or losing. This assertion is based on the results of articles published from the construction of the thesis, that is, sports determine and condition morphofunctional characteristics that are related to the demands of the sports environment; Athletes' previous experiences (victory or defeat) can determine subsequent results even in a short space of time; The training time and the morphofunctional factors are strong determinants for the outcome of the fights. These data corroborate and signal that the trainability seems to be one of the factors that differentiates winners and losers in the results obtained in this study.
Acesso aberto (Open Access)
Dissociação entre observação e interação na modificação do display agressivo do Betta splendens
(Universidade Federal do Pará, 2011) CUNHA NETO, João Soares da; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
The observation of fight (i.e., the presence of an anonymous observer of a fight) is something that changes the parameters of the fighting display of Betta splendens. The aim of this study was to identify whether and how observation alone or associated with the interaction modifies the emission of the display. Male Betta splendens were used (N = 28) in a battery of experiments to identify whether the aggressive display is modified either by the observation of a conspecific in a situation of confrontation (Experiment I) or by the observation of a conspecific in a situation of confrontation and subsequent exposure to this very conspecific (Experiment II). There was no statistical difference in the data obtained from the Experiment I, however, with respect to the data obtained in the Experiment II we could identify a statistically significant difference (F (1.6) = 6.002, p = 0.05) reflected in an increased latency of the Horizontal Display. We concluded that in Experiment I the behaviors were stable, and that the observation associated with interaction (Experiment II) is able to modify the emission of the aggressive display of Betta splendens.
Acesso aberto (Open Access)
Efectos de la obesidad y de la cirurgía bariátrica sobre patrones cognitivos y perceptuales de los humanos
(Universidade Federal do Pará, 2019-01-28) ESPEJO, Cristian Ariel Neira; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
Obesity is defined as an abnormal or excessive accumulation of fat that can be harmful to health and has been considered epidemic, since it is a disease with high prevalence in most countries of the world. Currently there is the idea that deficiencies in cognitive functions associated with the frontal lobe, contribute to the maintenance of obesity, however, there are investigations that contradict this postulate. On the other hand, the patterns of visual perception, measured with the Rorschach test, have been linked to weight gain and obesity, the results that determined this relationship are not conclusive. The main objective of this study was to determine if obesity or bariatric surgery generate differences in the patterns of visual perception and humans cognitive functioning. Additionally, we sought to relate the neuropsychological functions of the cortex occito-temporo-parietal, which participate in the perception of visual stimuli, with the neuropsychological functions of the prefrontal cortex. The research included the participation of 48 subjects, divided into 3 groups (diagnosed with obesity, undergoing bariatric surgery and a control group), which were evaluated with the Rorscahch test and the Stroop and MCSTneuropsychological tests. Our results showed similar values for the three groups in the measurements made with the neuropsychological tests and with the visual perception test. In addition, three indicators of the visual perception test were correlated with indicators of sustained attention, inhibitory control and cognitive flexibility. Our main conclusion is that the obese or bariatric subjects do not present differences in cognitive functioning or visual perception patterns. We also conclude that there are indicators of the Rorschach test that present a potential to be considered neuropsychological indicators.groups in the measurements made with the neuropsychological tests and with the visual perception test. In addition, three indicators of the visual perception test were correlated with indicators of sustained attention, inhibitory control and cognitive flexibility. Our main conclusion is that the obese or bariatric subjects do not present differences in cognitive functioning or visual perception patterns. We also conclude that there are indicators of the Rorschach test that present a potential to be considered neuropsychological indicators.
Acesso aberto (Open Access)
Efeito do ganhar ou perder nos niveis de raiva e ansiedade em lutadores de judô
(Universidade Federal do Pará, 2017-02-17) ARDILA, Héctor Andrés Páez; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
Aggression is a behavior that involves a simultaneous activation of physiological, biochemical, neurological and behavioral components and emotions, such as anxiety and anger. In humans, sports can be considered as a form of display because they allow aggression to be expressed with a low probability of permanent damage to subjects. The competitions have been used as models to evaluate the activation produced by the different stages of competition, such as the outcome of the combat. Judo has been used as a model of competitive aggression to evaluate the different body responses in agonistic behaviors in humans, since it offers a context like those studied in animal fights. The aim of this study was to evaluate the effect of win / lose in the levels of anger and anxiety in regional judo fighters, male, linked to the Para Federation of Judo, Using the STAXI and IDATE psychometric scales, as well as comparing these results with the Brazilian general population and a correlation analysis to know the differences between the different components with the number of strokes, using a pre/post fight evaluation and the filming of the fights. Differences were found between winners and losers, as well as between fighters and the Brazilian population; The losers presented higher levels of anger, while the anxiety was greater for the winners.
Acesso aberto (Open Access)
Efeitos da dominância sobre as dimensões de personalidade em Danio rerio
(Universidade Federal do Pará, 2016-05-13) PINHEIRO, Marcelo de Sena; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
The zebrafish is widely used in various areas of research as experimental model, dominance and submission phenomenon are present in this species, and relate directly with the aggressive behavior, these can be linked to personality dimensions that can be used to predict and explain the conduct of an individual, creating different profiles of behaviors on certain aspects, these behavioral changes may reflect several factors, among them bodily differences, the availability of food, the pressure of predators, the intraspecific competition, among others. To try to answer whether dominant subjects respond differently in situations involving appetitive and aversive stimuli, was performed a battery of tests involving both previously tested to determine its state of dominance, then were subjected to the following tests as scooling, predator inspection and open field. These results showed that the dominance profile in zebrafish, appears to be related to differences in the behavioral patterns when associated with possible parameter approximation of aversive stimuli (predator) and not aversive stimuli (shoals), which may be related to two large personality, extroversion and neuroticism.
Acesso aberto (Open Access)
Estimulação transcraniana por corrente contínua em esportes de combate: efeito sobre o desempenho físico e cognitivo
(Universidade Federal do Pará, 2021-11) LOBÃO, Thais Alves; GOMES, Bruno Duarte; http://lattes.cnpq.br/4932238030330851; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
Transcranial direct current electrical stimulation (tDCS) is a non-invasive neuromodulation technique used to promote improvement in clinical symptoms of neurodegenerative diseases. There is evidence that tDCS could modulate the psychomotor abilities of athletes, which are important for the performance of these athletes. Among the combat sports, judo and jiu-jitsu are two types that represent fights with specific physical and cognitive demands. Thus, the aim of this study was to investigate the effects of a single stimulation in the M1 region (primary motor), on cognitive (anxiety, reaction time) and physical (strength, muscle power, flexibility) parameters in federated male athletes of these modalities. For that, the fighters were submitted to two experimental sessions of tDCS (sham and stimulated, 2 mA for 20 minutes), in a cross-over scheme (ABA), composed by psychomotor evaluations using as measures the jump against movement (JAM), Wells bank, manual and scapular dynamometry, IDATE (state-trait anxiety index) and reaction time, through the TReaction software. There were no statistically significant differences between pre- and post-stimulation conditions (sham or anodic) and in the percentage of difference between the two pre- and post-test conditions. Our results indicate that there are no effects of tDCS in this region and in the values used on the measured parameters.
Acesso aberto (Open Access)
Parâmetros da escototaxia como modelo comportamental de ansiedade no paulistinha (danio rerio, cyprinidae, pisces)
(Universidade Federal do Pará, 2011-02-24) OLIVEIRA, Caio Maximino de; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
Certain teleost fishes present a behavioral trait of scototaxis, the preference for dark environ- ments and not bright ones. The present work tried to evaluate some parameters of the explora- tory behavior of the zebrafish (Danio rerio Hamilton 1822) in the black/white tank, aiming to establish the reliability of measures in different contexts. White compartment avoidance presents a biphasic pattern, with an increase in avoidance preceding a decrease (Experiment 1). This same avoidance does not habituate to repeated exposure, independently of the inter- session interval, on the contrary of total locomotion (Experiments 2 and 3); forced exposure to the white compartment does not alter the subsequent exploratory behavior (Experiment 4). These results suggest that novelty is not the controlling dimension of scototaxis; besides, these results also suggest that the preference for the Black compartment is not caused simply by avoidance of the white compartment, although this certainly has an important role. The role of the aversiveness of the white compartment was assessed in a second series of experi- ments. The illumination above the white compartment is an anxiogenic factor, since increas- ing it decreases the time spent in the white compartment without affecting total locomotion (Experiment 5). This phenomenon seems to be due to a decrease in the capacity to camouf- lage with the substrate (crypsis), since altering the color of the white compartment to grey increases its exploration, while altering the color of the black compartment to grey increases the time spent in the white compartment (Experiment 6). Besides, the increase in the propor- tion of the apparatus occupied by the white compartment (from 50% to 75%) decreases the time spent in it (Experiment 7). These results suggest that the white compartment is aversive, and therefore the preference for darkness is not caused simply by positive reinforcing proper- ties of the black compartment. Taken together, the results of both series of experiments sug- gest that scototaxis results from an approach-avoidance conflict. Experiment 8 represents a common environmental manipulation which alters anxiety in rodents, environmental enrich- ment; here, animals raised in an enriched aquarium for two weeks present less white avoid- ance. It is concluded that scototaxis has good construct validity.
Acesso aberto (Open Access)
Pez-cebra (Danio rerio) como modelo de bienestar animal
(Universidade Federal do Pará, 2022-10) VASQUEZ, Jhon Jairo Buenhombre; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274; https://orcid.org/0000-0003-1710-9662
Zebrafish has become an important model for various disciplines such as neuroscience and the testing of new pharmacological molecules. Therefore, the way in which it is housed and the consequences of its accommodation on its well-being represent an important aspect to study. Very few studies have evaluated the effect of environmental enrichment on the welfare of zebrafish and its negative or positive impacts have yet to be elucidated. An important challenge is to establish the consequences not only of the presence of enrichment but also of its loss. Likewise, the form of presentation of a stimulus, either predictably or unpredictably, influences the physiological and behavioural responses of the animal. Therefore, the form of presentation of the enrichment represents another important aspect to assess. The evaluation of emotional (affective) states is crucial for the study of zebrafish welfare, in particular to evaluate the influences of the environment on the animal. However, directly assessing these states is difficult due to their subjective nature. Therefore, the current approach focuses on estimating them from the resulting physiological and behavioral components. Today there are robust standardized tests that assess and correlate neuroendocrine responses with well-established behavioural assessment criteria for stress and anxiety in zebrafish. However, there are no similar tests to measure positive emotional states in this species. An alternative metric to assess both the positive and negative affective states of animals is the cognitive bias. These tests allow researchers to infer an individual's emotional state from cognition. Therefore, the evaluation of apparently negative (Unpredictable conditions and absence or loss of enrichment) and positive ones (Predictable conditions and presence or gain of enrichment) accommodation contrasts through a type of cognitive bias (cognitive judgment bias (CJB) in zebrafish may constitute a promising model to understand the effects of housing on affective states and well-being in this fish. Additionally, it has been shown that individual variations such as personality influence the central nervous system, behaviour and cognitive judgment bias results in other species. These differences and their impacts on zebrafish have not yet been studied. Consequently, the general objective of this thesis was to establish if the cognitive judgment bias can be used in zebrafish for the evaluation of positive and negative emotional states produced by environmental modifications and its relationship with personality.
Acesso aberto (Open Access)
Preferência Claro/Escuro em Danio rerio: Efeitos da Melatonina Sobre o Horário da Coleta e de Regime de Luz
(Universidade Federal do Pará, 2014-12-12) DIAS, Cláudio Alberto Gellis De Mattos; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
Danio rerio is a widely used animal model for behavioral experiments in white / black tanks. The white side of the aquarium seems to cause aversion and the preference seems to be to stay longer on the dark side. Melatonin is a indoleamine which influence the physiological functioning of fish. The aims of this work are: to survey the literature on melatonin and its correlation with fishes and Danio rerio; compare the efficiency of two apparatuses insulation in behavioral tests; testing the influence of four periods of the day and five photoperiods response variation in Zebrafish to light-dark test; compare the time spent on each side of the aquarium test period, subjects isolated on a photoperiod of 12/12 hours with exposure to seven different concentrations of melatonin; statistically comparing the sample into quartiles within each concentration; and statistically compare the sample into quartiles among the first, second and third quartiles of each concentration. The Zebrafish and the fishes seem to have their physiology and behavior influenced by melatonin (both endogenous as exogenous). Both apparatuses isolation proved to be equally effective for behavioral tests. Luminosity seems to drag the Zebrafish circadian cycle, reducing their aversion to the white side of the aquarium, photoperiod 12/12 hour period and night collection. Melatonin seems to influence the change caused by photoperiod, decreasing or canceling the dragging. In our sample seems to be subpopulations that respond differently to exposure to the same concentration of melatonin. Studies with specific strains of Zebrafish and with a wider range of concentrations of melatonin appear to be necessary to identify the specific dosage possible to counteract the performance in light entrainment of the circadian cycle in Danio rerio.
Acesso aberto (Open Access)
A tomada de decisões no judô: o que se diz e o que se faz? Relação entre tomada de decisões e perfil neurocognitivo
(Universidade Federal do Pará, 2023-03) RIVERA, Luis Felipe Sarmiento; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274; https://orcid.org/0000-0003-1710-9662
There are two ways to think about how the decision process is done. The process can be automatic and having psychomotor characteristics, or mediated by linguistic process -in a deliberated way-. Moreover, martial arts bring an environment with high emotionality, where the anxiety is present. The objective of this study is to understand how the decision-making process, the anxiety and the impulsivity is presented in a combat physical activity, a Judo fight, that demands constant decisions and responses with limited time. To do this, there were to groups of participants (30 judo fighters and 15 non-fighters). One of the groups watch a video and anticipated the fighter’s movement with a questionary, and the other group was recorded in a judo fight. Both groups also performed an impulsivity test and an anxiety test. Each participant had more than 4,5 years of training and was an active competitor. There were found differences in the decisions made by the participants in the fight and the decisions that the participants told they would perform, specifically, when they were going to execute and action. Moreover, there was a significant difference in the anxiety levels of the group of fighters compared to controls, where the fighters had higher anxiety levels than controls. There were none significative differences in impulsivity between the groups. The results of the study shown how is cognition in Judo fighters, indicating where to focus the training and competition programs of the fighters.
Acesso aberto (Open Access)
Validação farmacológica da esquiva Inibitória do Danio Rerio
(Universidade Federal do Pará, 2013-06-12) SANTOS, Bruno Rodrigues; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
The Danio rerio is a fish species widely used in emerging models of anxiety due to well described behavior, anatomy, neuroanatomy and biochemistry. The inhibitory avoidance test is an anxiety-related parameters in the model development that described a suppressed behavior emitted to avoid an aversive stimulus. This experiment aimed to describe the inhibitory avoidance averssiveness of white side and the pharmacological validation of this test by using the following drugs: antidepressants - fluoxetine (5, 10 and 20 mg/kg) and imipramine (4, 8 and 16 mg/kg), anxiolytics - diazepam (0.06, 1.25 and 2 , 5 mg/kg) and clonazepam (0.02, 0.05 and 1.10 mg/kg), and stimulants - diethylpropion (2.5, 5 and 10 mg/kg) and caffeine (10, 20 and 40 mg/kg). The data confirm the aversiveness of the white side in inhibitory avoidance test. Flouxetine, imipramine, caffeine and dietilpropione have anxiogenic-like effect and imapaired inhibitory avoidance, excluding imipramine 8 mg/kg who facilitates its acquisition. Diazepam also facilitates inhibitory avoidance acquisition and clonazepam show anxiolytic-like effect on this test. These data show the effectiveness of inhibitory avoidance test as a model to study axiety.
Acesso aberto (Open Access)
Validação farmacológica da preferência claro-escuro em Danio rerio
(Universidade Federal do Pará, 2012-04-23) MAGNO, Lílian Danielle Paiva; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
Anxiety is a complex disorder with large clinical relevance, whose study with animal models is important for research about their mechanisms and drugs for their treatment. The zebrafish appears as a potential animal model for pharmacological research in anxiety. A model of anxiety is the light-dark preference, which has been validated behaviorally in zebrafish, however, requires a pharmacological validation. The objective is to describe the sensitivity of the light-dark preference in zebrafish adults for the most common drugs in clinical anxiety, were administered by immersing the animal in the solution: Benzodiazepines (Clonazepam), 5-HT1A partial agonists (Buspirone), Tricyclic Antidepressant (Imipramine), Antidepressant SSRIs (Fluoxetine and Paroxetine), Antipsychotics (Haloperidol and Risperidone); Psychostimulant (Diethylpropion), Beta blockers (Propranolol) and CNS depressants (Ethanol). The parameters analyzed were the time spent by the animal in a dark environment, the time of the first latency and number of midline crossings. Clonazepam administered 300 s increased the time in the dark at lower concentrations and reduced locomotor activity, administration during 600 s of the intermediate concentration decreased over time in the dark and the first latency, and increased locomotor activity, indicating anxiolytic effect. Buspirone raised the time spent in the dark, probably due to reduction of motor activity. Imipramine and fluoxetine increased time in the dark and the first latency and decreased the number of alternations, indicating anxiogenic action. Paroxetine did not alter the time in the dark, however the first time increased latency and decreased locomotor activity. Haloperidol decreased anxiety in the lowest concentration, curiously raised motor activity at the highest concentration, instead of risperidone, which decreased the activity at the highest concentration. Diethylpropion did not change over time in the dark but increased the time of the first latency and decreased motor activity only at lower concentrations. Propranolol reduced only time in the dark. Ethanol was effective in reducing anxiety with the intermediate concentration and decreased locomotor activity in a lower concentration. Data corroborate with the literature in Danio rerio both intraperitoneal administration in this model as in other models for water delivery and in rodents, when it was possible to compare.