Navegando por Orientadores "MACHADO, Luiz Fernando Almeida"

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Acesso aberto (Open Access)
Estudo epidemiológico de agentes virais (HIV, HTLV, VHB e CMV) identificados em adolescentes grávidas atendidas em um centro de referência do Sistema Único de Saúde de Belém, Pará
(Universidade Federal do Pará, 2010-11-05) GUERRA, Aubaneide Batista; MACHADO, Luiz Fernando Almeida; http://lattes.cnpq.br/8099461017092882
The prevalence of infection by viral agents such as HIV-1, HTLV1/2, VHB and CMV is not so much known in pregnant teenage population from the North region of Brazil. One of the objectives of this study was to describe the prevalence of infections HIV-1, HTLV1/2, VHB and CMV in pregnant adolescents, attended in a Reference Center in the State of Pará. To achieve the objectives, it was collected blood samples from 324 pregnant adolescents, who came from several cities of the State, from November of 2009 to February of 2010. The samples were submitted to an immuno-enzymatic test (ELISA), in order to detect antibodies Anti- HIV, Anti- HTL V1/2, Anti- VHB and Anti- CMV IgM/ IgG. The serological analysis revealed one serum positive sample for HIV-1, two positives samples for HTLV1/2, while the majority of samples showed antibodies Anti-CMV-IgG, although the occurrence of high infection was low (2.2%). The prevalence of infection for VHB in pregnant adolescents was 0.62%, however a great number (83.3%) of adolescents are susceptible to the infection by VHB, what means they probably were not immunized. The majority of adolescents (63.4%) continued their studies, even though they know about their pregnancy and 34.6% started prenatal later, so they only had a minimum number of four consultations of prenatal. The result reinforces the hypothesis that pregnant adolescents have prevalence for those viral type infections, what is similar to the national pregnant women rates. The prevalence of viral subtypes (HTLV-2 and HIV-1 subtype B) obtained by the molecular characterization of serum positive samples from pregnant adolescents agreed with the prevalence of subtypes from the North region.
Acesso aberto (Open Access)
Prevalência da infecção pelo Papilomavírus humano (HPV) em mulheres investigadas para o câncer cervical, na cidade de Belém, Para
(Universidade Federal do Pará, 2009-04-29) MONTEIRO, Jacqueline Cortinhas; MACHADO, Luiz Fernando Almeida; http://lattes.cnpq.br/8099461017092882
The human Papillomavirus infects basal cells of stratified epithelium, inducing the development of benign proliferative lesions in the skin or mucous membranes. The infections have universal distribution. Many studies have demonstrated the strong association of infection by species of high-risk cervical cancer. Therefore, this study sought to determine the prevalence of HPV infection in a group of women screened for cervical cancer. From january 2008 to december 2009 were collected cervical samples from 180 women seen at the Laboratory of Cytopathology of Universidade Federal do Pará (UFPA), with 162 eligible for the study. Each participant was collected two samples, one for the manufacture of cytological smears for subsequent analysis by the Papanicolaou method and the other for analysis of molecular biology, in order to investigate the presence of HPV, using the primers MY09 and MY11. The species of HPV have been identified by PCR sequencing of nucleotide bases of the L1 ORF of HPV. The analysis of the epidemiological profile of the study group showed that the average age accounted for 37.5 years. The majority (49.38%) were married, 37.65% had initiated sexual activity between 13 and 17 years old and 58.64% did not use condoms during sex. The overall prevalence of HPV was found to be 18.52%. Thirteen different types were identified, with the majority (66.67%) was a low-risk group, in which HPV-11 was more frequent. Among the high risk group (30%) HPV-31 was found in four cases. The species distribution of HPV according to the cytology showed that the prevalence of infection was higher in the group of women whose smear was free of abnormal cytology (43.33%). In the group of women with premalignant changes only observed infections by species of low risk (10%). HPV infection was significantly associated with sexual activity and the frequency in the completion of screening. The prevalence in this study corroborates other findings in the medical literature. The prevalence of infection in women with normal cytology reinforces the idea that the infection is mostly asymptomatic and the Papanicolaou method is less efficient in detecting the infection than the techniques of molecular biology. However, when dealing with disease detection premalignant or malignant, molecular biology is not applicable and therefore should not replace the PCCU.
Acesso aberto (Open Access)
Soroprevalência e caracterização molecular do Vírus da imunodeficiência humana (HIV) e do Vírus linfotrópico de células T humanas (HTLV) em mulheres profissionais do sexo do Estado do Pará, Brasil
(Universidade Federal do Pará, 2007-08-03) SOUSA, Ronaldo Lopes de; MACHADO, Luiz Fernando Almeida; http://lattes.cnpq.br/8099461017092882
The Women’s Professional of the Sex (WPS) constitute a vulnerable population to the acquisition of sexually transmissible illnesses (STI) in virtue of the type of activity that they play. The present work had as objective to describe the serumprevalency of the infection and the molecular characterization of the HIV and the HTLV of WPS of the State of Pará, Brazil. Blood sample’s of 339 WPS were collected, being 105 proceeding from the city of Barcarena, 31 of the city of Augusto Correa, 98 of the city of Bragança and 105 of the city of Belém, in the period of April of 2005 the August of 2006. All the plasma samples had been tested for the presence of antibodies anti-HTLV-1/2 and anti-HIV-1/2, by means of the use of a immune enzimatic assay. The prevalence of the HIV-1 and the HTLV-1 in the studied population was of 2,36% and 1,77%, respectively. The amplification of the gene pro of the HIV-1 disclosed subtypes B and F, whereas the samples of HTLV, had been classified as HTLV-1 of the Cosmopolita subtype of the Transcontinental sub-group. It had a significant association of the infection for the HIV-1 with the age band, the number of partners per week and with the capricious use of condoms. However, the infection for the HTLV-1 alone showed significant association in relation to the capricious use of condom. The sexual relation was the most likely way of acquisition of the infection for the two viral agents, considering that the report of use of injective drugs was rare. Being thus, the WPS need bigger attention of the public agencies of health, specially in relation to the prevention and monitoring of transmissible agents by sexual pathways, as these can be important in the acquisition and dissemination of these agents.