Navegando por Orientadores "MAIA, Cristiane do Socorro Ferraz"

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Alterações no comportamento motor e na bioquímica oxidativa de ratas intoxicadas agudamente com etanol no período inicial da adolescência
(Universidade Federal do Pará, 2015-01-30) CERASI JUNIOR, Antonio José; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
Ethanol (EtOH) is one of the most commonly psychoactive substances consumed by young people, usually occurring in episodes of binge consumption, which is an intense form of drinking followed by a period of abstinence. In Brazil, the rates of alcohol abuse by female adolescents have increased in the past few years. This population is more sensitive to the neurotoxic effects of this substance since the complete development of structures such as the cerebellum occurs in this stage of life. Acute and chronic consumption of ethanol and its catabolism are associated with motor behavior alterations so as the generation of reactive oxygen species (ROS) and other metabolites. The purpose of this study was to investigate the effects of EtOH exposure following an acute binge pattern in female rats on their early adolescence (n = 20, n = 10 animals per group) received distilled water or EtOH (3 g / kg / day, 20 w / v) by gavage, for 3 consecutive days. Seven and a half hours after the last day of administration, the behavioral tests of spontaneous locomotor activity and rotarod were performed. Subsequently, the animals were sacrificed for cerebellum removal and the oxidative biochemical tests in cerebellar tissue were carried out. Behavioral tests showed that acute binge in rats on early adolescence did not alter spontaneous locomotor activity, but elicited alterations in motor learning. Oxidative biochemical results showed a high activity of catalase, which demonstrates that there was an offset of the body to reverse the ROS production in the cerebellum of adolescent rats acutely exposed to EtOH followed by a withdrawal period (binge).
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Avaliação das alterações cerebelares decorrentes da exposição ao etanol da adolescência à fase adulta
(Universidade Federal do Pará, 2014-05-07) CUNHA, Polyane Alencar; LIMA, Rafael Rodrigues; http://lattes.cnpq.br/3512648574555468; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
Alcohol abuse has reached massive proportions, associated with a number of adverse consequences, becoming one of the most widespread phenomena of recent decades and therefore alcohol, alcoholic disease agent of a public health problem. Young people are under physiological and social transition, with the curiosity, the pressure of the social group, the family model, propaganda and lack of public policies, the main risk factors which lead to early alcohol consumption. The teenager once is maturing presents its central nervous system (CNS) vulnerable to aggression caused by the consumption of this drug. It is known that alcohol promotes a series of motor change the point of view of clinical and physiological concurrently. These amendments are consequential action of alcohol sore CNS, specifically on the Cerebellum, and by chronic use of the drug suffer (like other organs of the CNS) some changes. The aim of this study was to evaluate the cerebellar changes resulting from exposure to ethanol from adolescence to adulthood in rats, on the morphometric patterns observed in the organ, tissue response and investigation of oxidative balance the body against the injury caused by toxic. Mice received from the 35th day of postnatal life (puberty period) by gavage at a dose of EtOH 6.5g/kg/day for 90 days (adulthood). To view the injury caused to the fabric was made morphometric biochemical research and analysis through oxidative stress. The results show that cerebellar mass is not changed, but there is a reduction in organ size and an increase in the density of the cell population in the molecular and granular layers, however a decrease in neuronal cells, with all the changes occurred were not morphohistologics accompanied by alterations in the biochemical oxidation.
Acesso aberto (Open Access)
Avaliação do efeito neuroprotetor da cafeína em ratas intoxicadas por etanol no padrão binge
(Universidade Federal do Pará, 2019-05-03) BARROS, Mayara Arouck; FERNANDES, Luanna de Melo Pereira; http://lattes.cnpq.br/0156144290849777; https://orcid.org/0000-0001-7004-0719; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
Ethyl alcohol is a substance consumed since the dawn of mankind, extending to the present day. In this context, adolescents are attracting attention because consumption in this group is constantly rising, especially in the Binge Drinking pattern. This model of consumption leads to functional impairments, such as symptoms associated with anxiety and depression. For this reason, has been sought bioactive compound to prevent such damages, among them we can highlight the caffeine, substance widely consumed in the world. In view of this, this paper aims to investigate the neuroprotective effect of caffeine in self administration on the damages caused by EtOH intoxication in the binge pattern, aiming at an alternative to minimize the neurotoxic damages promoted by alcohol with the simple habit of drinking coffee. For this, Wistar rats, females, age 35 days (n = 40) were used. At treatment, animals received caffeine repeatedly from the 35th to the 72nd postnatal day (PND). Caffeine was administered as a 0.3g / L caffeinated solution in self-administration in the active period, starting 14 days before the first day of ethanol intoxication and persisting during the 4 weeks of BD. For ethanol intoxication in the binge pattern, it was administered by gavage at a dose of 3 g / kg / day (20 w / v) for three consecutive days weekly in the animals from the 49th to the 72nd PND. For behavioral tests, the animals were exposed to Open Field, Elevated-plus-maze, Splash and Forced swimming test. In relation to the anxious type behavior, the Caffeine + EtOH Group showed a significant increase in the distance and time traveled in the central area in the Open Field Test when compared to the EtOH group. In the LCE Test, the EtOH group showed reduction in %EBA and %TBA when compared to the control group. However, the caffeine group showed reversion when increasing both parameters when compared to the EtOH group. In the depressive-type behavior, the EtOH-intoxicated group presented reduction of the grooming time and increased immobility time, in the Splash and Forced Swim tests, respectively. The EtOH + Caffeine group was able to increase grooming time in the first test and reduce immobility time in the second. Thus, we can conclude that chronic consumption of caffeine promoted neuroprotection by reversing symptoms similar to anxiety and depression
Acesso aberto (Open Access)
Avaliação do óleo essencial de pau-rosa (Aniba rosaeodora Ducke) em modelo de depressão induzida por álcool em ratas adolescentes
(Universidade Federal do Pará, 2023-08) SANTOS, Éverton Renan Quaresma dos; MAIA, José Guilherme Soares; http://lattes.cnpq.br/1034534634988402; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
Depression is a prevalent disorder worldwide, which affects the functionality and quality of life of the people. The linalool-rich rosewood essential oil (Aniba rosaeodora Ducke) displays activities on the central nervous system (CNS), including of antidepressant-type. The work aimed to evaluate the effect of the essential oil in adolescent female rats through a model of binge drinking-induced depression. The essential oil was extracted by hydrodistillation and analyzed by a dual system of gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (CG-FID). The in vitro antioxidant capacity of the essential oil was determined in the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging assay. Female Wistar rats, 29 days old, received distilled water or ethanol (3 g/kg/day) orally in 4 binge episodes, and saline solution or rosewood oil (35 mg/kg) intranasally once a day for 28 days. After the experimental treatment, splash and forced swimming tests were performed. The brain-derived neurotrophic factor (BDNF) and S100B gene expression, biological parameters of the oxidative stress and levels of pro-inflammatory cytokines were determined in the prefrontal cortex and hippocampus. The OEPR+EtOH group increased self-cleaning time and decreased immobility time, both significantly compared to the EtOH group in the splash and forced swimming tests, respectively. The essential oil behavioral effects were related to positive modulation of BDNF and S100B genes, to restoration of glutathione (GSH) and Trolox equivalent antioxidant capacity (TEAC) levels, in addition to the attenuation of the increase of thiobarbituric acid reactive species (TBARS), Interleukin-1β (IL-1β), Interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) induced by alcohol. The results suggest that essential oil improved the alcohol-induced depressive profile through a neuroprotective mechanism by positively modulating the gene expression of neurotrophic factors, rebalancing the antioxidant status and attenuating the inflammatory process, possibly due to the linalool action.
Acesso aberto (Open Access)
Avaliação dos efeitos no sistema nervoso central e estresse oxidativo do extrato hidroalcoólico de Petiveria alliacea L. (Phytolacaceae)
(Universidade Federal do Pará, 2011) ANDRADE, Thaís Montenegro de; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
Acesso aberto (Open Access)
Avaliação neurocomportamental, antinociceptiva e antioxidante do extrato hidroalcoólico de Eupatorium ayapana Vent (Asteraceae)
(Universidade Federal do Pará, 2012-03-30) MELO, Ademar Soares; SOUSA, Pergentino José da Cunha; http://lattes.cnpq.br/9909053957915090; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
The Eupatorium ayapana Vent., Family Asteraceae, popularly known as japana is used in infusions, decoctions, baths and tea, with sedative, febrifuge, stimulant and tonic, also used to combat insomnia, headache, sore throat, diarrhea, etc.., commonly used by the Amazonian population. This study evaluated the effect of different doses of hydroalcoholic extract of Eupatorium ayapana Vent (EHAEA) on the behavior of Wistar rats, aged 2 months. We used eight groups of rats (n = 7-10) that were divided into control, standard drug action anxiolytic (diazepam), standard drug action antidepressant (fluoxetine) and five doses of the extract (100, 200, 400, 600, 800 mg/kg) which were solubilized with 1% tween 80. The administration of the extract was performed acutely by gavage. In oral toxicity test, it was found that the extract is not toxic. The behavioral tests were used: the open field, the elevated plus maze (EPM) and forced swimming. After the behavioral tests was performed to collect blood in the retro-orbital plexus of rats to assess the levels of oxidative stress such as Total Antioxidant Capacity, MDA and NO, and also the total antioxidant EHAEA. The results obtained in the open field test showed anxiolytic-like activity, a result confirmed in the LCE. In the forced swimming test, the EHAEA showed antidepressant action of any kind. In tests of nociception, which was used mice occurred in antinociceptive abdominal writhing test in doses of 200, 400, 600 and 800 mg/kg. In evaluating the biochemical oxidation, it was observed that there was no oxidative damage in the treated groups EHAEA, NO levels were unchanged at doses of 200, 400 and 600 mg total antioxidant capacity was shown to be increased. With these results, this paper aims to contribute to future work, have seen, are few studies in the behavioral area, in nociception and oxidative stress in this plant species, and that further studies may enhance the use of the extract in folk medicine japana.
Acesso aberto (Open Access)
Caracterização dos efeitos comportamentais, teciduais e bioquímicos da administração intermitente e episódica de EtOH em ratas da adolescência à fase adulta
(Universidade Federal do Pará, 2016-10-18) FERNANDES, Luanna de Melo Pereira; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
The consumption of ethanol (EtOH) is enhanced particularly in adolescent female pubic. The EtOH intake and intermittent episodic own consumption rate around 3 times per week. The toxic effects of this kind of consumption is especially dangerous over the continuous consumption of EtOH followed due to the high dietary intakes of abstinence, causing major changes in the central nervous system (CNS) maturing in a short time consumption. Considering the epidemiological relevance and the harmful effects of EtOH on the oxidative balance, hormone production and neurotrophin CNS maturing, the aim of this study was to investigate the behavioural, tissue and biochemical responses derived from intermittent and episodic consumption of EtOH in rats in phase from adolescence to adulthood. Wistar female adolescents (n = 80) received by gavage, distilled water or EtOH (3 g/kg/day) for 3 consecutive days per week. The animals were assessed seven and a half hours after the last administration day 1, 4 and 8 weeks of episodes of binge drinking (37, 58 and 86 DPN, respectively), besides, a period of 14 days of abstinence was added after BD 8 (100 DPN) to evaluate the ability to reverse the CNS damage generated on it. The battery of behavioural tests consisted of spontaneous locomotor activity, object recognition, elevated plus maze, test pole, walking beam and rotarod. The animals were sacrificed and blood samples collected for evaluation of corticosterone levels of malondialdehyde, catalase activity, the activity of superoxide dismutase and glutathione content. Therefore, the hippocampus was dissected to quantify the immunocontent BDNF. The administration of EtOH reached average peak blood concentration of 197.4 mg / dL and the period of 7.5 hours after the last administration EtOH in acute binge blood concentration was 0.7 mg / dL. Thus, the animals underwent behavioural tests post-consumer EtOH, not under the drug effect. Consumption of EtOH in binge did not affect weight gain of adolescent animals into adulthood, however, reduced the exploratory locomotor activity, impaired motor coordination, balance and motor learning associated with bradykinesia, as well as loss in the mnemonic process and increased anxiety-like behaviour. These losses were accompanied by hormonal elevation of corticosterone, reduced hippocampal BDNF levels and systemic imbalance in the oxidative balance. Thus, it was possible to identify that the damage found on the similar behaviour to anxiety, short-term memory, bradykinesia and spontaneous locomotor activity appeared from EtOH post-consumption for three consecutive days, however, they showed no recovery or worsening of damage after repeated episodes. In contrast, there was recovery of short-term memory in object recognition task associated with the return of normal levels of BDNF in adulthood. Moreover, it showed worsening in motor learning in young adult phase followed by gradual and partial recovery after prolonged period of drug withdrawal, yet the loss of motor coordination and balance remained in adulthood.
Acesso aberto (Open Access)
Efeitos da exposição crônica ao etanol da adolescência à fase adulta em ratos sobre o processo mnemônico e na densidade celular no hipocampo
(Universidade Federal do Pará, 2012-11-30) OLIVEIRA, Maria Cristina Souza Pereira; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
The central nervous system is vulnerable to the action of several substances including these are psychoactive substances such as ethyl alcohol or ethanol, which is consumed by mankind for a long time and is associated with a range of medical social problems. Therefore, the objective of this study was to investigate the neurobehavioral alterations and tissue from exposure to ethanol from adolescence to adulthood on the hippocampus. For this, 30 Wistar rats were evaluated distributed into control and ethanol, which upon reaching 35 days of age (adolescence) received by gavage ethanol at a dose of 6.5 g/Kg/day for 90 days (adulthood ). After this period, the animals were subjected to behavioral tests of open field, social recognition and avoidance for evaluation and subsequently perfused tissue through histological processing by violet cresila. Were collected and processed sections of 50 μm, the samples were subjected to staining violet cresila. The results demonstrate a deficit in the parameters measured in the behavioral assays such as impairment in natural ambulation of the animal, the percentage of the number of quarters and time in the central core area which suggests anxiogenic-like behavior, increase in exploratory activity surveying related to animal. Observed impairment in the development of mnemonic processes with reduced memory retention of short and long term as well as in social recognition. Regarding the treatment histologically observed decrease in cell density in CA1 and CA3 regions of the hippocampus. These results indicate that chronic exposure to ethanol from adolescence to adulthood disrupts behavioral aspects related to the limbic system, the hippocampus where it entered.
Acesso aberto (Open Access)
Efeitos neurocomportamentais e no estresse oxidativo em ratos tratados com extrato etanólico de própolis amarela
(Universidade Federal do Pará, 2015) SILVEIRA, Cinthia Cristina Sousa de Menezes da; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
Propolis is a resinous substance produced by bees that collect raw material from different parts of plants, through the addition of salivary secretions and wax. In Brazil, 13 types of propolis were chemically characterized. In the yellow propolis of Mato Grosso do Sul were identified 15 compounds, all belonging to the class of triterpenoids, and low levels of phenolic compounds and flavonoids. This work aims to conduct behavioral and biochemical assays with acute administration of yellow propolis ethanolic extract. 8 groups of male Wistar rats, 3 months, were used (n = 10 per group) and were divided into control (Tween 5%), positive control for anxiolytic activity (diazepam), positive control for antidepressant activity (fluoxetine), positive control for mnemonic effect (caffeine), 4 doses of the extract (1, 3, 10, 30mg/kg). The extract administration was performed acutely, intraperitoneally. Behavioral tests were open field, elevated plus maze, forced swimming and inhibitory avoidance. After the behavioral testing was performed to collect blood in the intracardiac area of the animals for determination of nitric oxide, malondialdehyde, catalase, superoxide dismutase and total antioxidant capacity. The results obtained in the open field test showed spontaneous locomotion preserved and anxiolytic-like activity, confirmed result with the elevated plus maze. In the forced swimming test, the yellow propolis ethanolic extract demonstrated action of antidepressant-like. In the inhibitory avoidance test showed mnemonic activity at 30 mg/kg. In the evaluation of oxidative biochemistry, the extract reduced the production of nitric oxide and malondialdehyde without changing level of total antioxidant, catalase and superoxide dismutase, induced by stress. With these results it is concluded that the yellow propolis ethanolic extract has anxiolytic, antidepressant, mnemonic and antioxidant activity.
Acesso aberto (Open Access)
Efeitos no comportamento motor após intoxicação subcrônica de metilmercúrio na presença de etanol (padrão binge) em ratas adolescentes à fase adulta
(Universidade Federal do Pará, 2015-11-06) OLIVEIRA, Aline do Nascimento de; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
Exposure to methylmercury through the contaminated seafood diet, concomitant abusive alcohol intake, in binge pattern, is quite common in gold mining communities of gold extraction, especially in the Amazon. The association between these two neurotoxicantes is also evident among adolescent women and creates the need to understand its effects on the central nervous system, especially in motor coordination, balance and spontaneous locomotion, because the studies are advanced only for the effects of exposure in isolation. Therefore this study aims to evaluate the effects on motor behavior resulting of subchronic exposure to methylmercury in the presence of alcohol, in binge pattern, in adolescents female rats until early adulthood (37-72 postnatal day), through behavioral motors tests, like Open Field, Pole Test, Rotarod and Beam Walking Test. The testing occurred 24 hours after the last intoxication of rats, which received methylmercury (0.04 mg / kg / day) for 35 days, concomitant with alcohol (3g / kg / day), 3 intermittent days, 1 time per week (binge), totaling 5 binges. The results showed a decrease in spontaneous locomotion in Open Field test through the parameters of the total distance traveled and number of rearing. In the Pole test was increased fall time, evidencing the bradykinesia. In the Rotarod there was a decrease in latency in the first three exhibitions, as well as Beam Walking Test was increased latency and number of slips, especially in thinner beams, showing that subchronic exposure to methylmercury in the presence of alcohol, in the binge, in adolescent female rats was able to produce behavioral damages related to coordinating motor, balance and spontaneous locomotor activity.
Acesso aberto (Open Access)
Investigação dos efeitos da cafeína e SCH58261 sobre as alterações comportamentais e no estresse oxidativo, e papel dos receptores A2A na potenciação de longo prazo após intoxicação por etanol em padrão binge em ratos fêmeas da adolescência a fase adulta
(Universidade Federal do Pará, 2022-11) PINHEIRO, Bruno Gonçalves; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
Introduction: Binge consumption of ethanol is an intermittent and episodic pattern of ingestion involved in several brain disorders that affect adolescents, considered more susceptible to damage that persists into adulthood. In the deleterious effects of ethanol, an important intoxication mechanism is the overproduction of adenosine, which causes hyperexcitability in its receptors, generating behavioral changes and oxidative stress. These receptors are antagonized by caffeine, a bioactive compound that can modulate the deleterious overactivation of ethanol. Objective: The aim of this study was to investigate the effects of caffeine administration on behavioral changes related to locomotion, anxiety, cognition and oxidative balance induced by ethanol in the binge drinking pattern during adolescence. In addition, it aims to assess the contribution of A2A receptors in the observed changes, including long-term potentiation (LTP). Material and Methods: Female Wistar rats (35 days old; n = 102) were allocated into six groups: control (distilled water, v.o), ethanol (3 g/kg/day; 3 days on-4 days off, v.o) , caffeine (10 mg/kg/day, v.o), caffeine + ethanol, A2A antagonist SCH58261 (0.1 mg/kg/day, intraperitoneal - i,p) and ethanol + SCH58261. The animals were submitted to open field behavioral tests, object recognition and elevated plus maze. The oxidative biochemistry parameters of trolox equivalent antioxidant capacity (TEAC), glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) in the pre- frontal and hippocampus. LTP recordings in the medial prefrontal cortex (mPFC), ventral (vHip) and dorsal (dHIP) portions of the hippocampus of the control, ethanol, ethanol + SCH58261 and SCH58261 groups were evaluated through electrophysiology. Results: Caffeine prevented ethanol-induced behavioral impairments, including by blocking A2A receptors. In addition, it attenuated the oxidative stress induced by binge drinking by alternative A2A receptor pathways. Blockade of A2A receptors increased LTP levels in mPFC and vHIP, however decreased in dHIP. Conclusion: Caffeine showed neuroprotection in behavioral changes and oxidative stress induced by the binge drinking model in adolescent rats. In addition, blockade of A2A receptors mitigated the observed behavioral changes, with improvement of LTP levels in the prefrontal cortex and hippocampus, which suggests the contribution of this pathway to neuroprotection in deficits induced by ethanol exposure during adolescence.
Acesso aberto (Open Access)
Investigação dos prejuízos motores decorrentes da exposição crônica ao etanol, em ratas intoxicadas da adolescência à fase adulta
(Universidade Federal do Pará, 2013-09-19) SILVA, Fernando Bezerra Romualdo da; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
According to the World Health Organization, ethanol is the most widely used psychoactive drug in the world, occupying a prominent place by now be considered a public health problem worldwide. His heavy drinking among adolescents is on the rise, from 15 years and young adults A wide variety of research related to its damaging many organs and body systems effects, as well as damage and degeneration of the central nervous system structures, and the cerebellum one of its main targets is the affinity of the substance for cellular structures is the ability to generate a cascade of deleterious processes . The aim of this study was to evaluate behavioral changes associated with the cerebellum in rats chronically intoxicated with ethanol adolescence to adulthood. Female at 35 days of postnatal life received during 55 days, ethanol by gavage. After this period of intoxication, the animals underwent a battery of tests to assess motor deficits. Behavioral tests consisted Open Field, Pole Test, Walking Beam and Rotarod. The results showed that animals exposed to ethanol displayed motor deficits in all behavioral tests. Although several mechanisms are associated with cerebellar degeneration, further studies should be conducted to investigate the true effect of this drug and their correlation wiser the results obtained in this research.
Acesso aberto (Open Access)
Minociclina atenua os prejuízos motores em ratos submetidos à isquemia focal no córtex motor e expostos cronicamente ao etanol da adolescência à fase adulta
(Universidade Federal do Pará, 2012-08-30) OLIVEIRA, Gedeão Batista de; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
According to World Health Organization, alcohol consumption in the world has become a public health problem. In this context, Brazil is at 63th position in the world in per capita alcohol consumption for people aged 15 or older. In addition to its socio-economic effects, ethanol is an important risk factor in the occurrence of cerebral ischemia. Exacerbated consumption of this drug contributes to the poor prognosis, as well as possible treatment for health problems related to stroke. The objective of this study was to evaluate neuromotor changes after blocking micróglial activation with minocycline in rats subjected to focal ischemia in the motor cortex, when treated chronically with ethanol from adolescence to adulthood. Rats were given for 55 days by gavage ethanol (6.5 g/kg/dia, 22.5 w/v). At the end of 55 days the animals were microinjected with the vasoconstrictor peptide endothelin-1 (40 pmol) for induction of focal ischemic lesion. The ischemic animals were treated with minocycline receiving two daily doses of 50 mg/kg in the first two days, and five daily applications of single 25mg/kg, intraperitoneally, by the seventh day post-ischemic induction. Behavioral tests consisted of open field, inclined plane and rota-rod. The results showed that the animals were exposed to ethanol showed motor deficits in all behavioral tests. However, treatment with minocycline was able to reverse them, enabling better performance on all tests. The results suggest that minocycline was able to reverse damage caused by motors stroke, even in the presence of ethanol. The exact mechanism involved in this process need to be investigated in future research.
Acesso aberto (Open Access)
Nocicepção em prole adulta de camundongo em exposição à morfina no período gestacional e lactação
(Universidade Federal do Pará, 2014-10-18) CASTRO, Nair Correia de Freitas; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
The use of morphine as a drug of abuse during pregnancy and lactation induces effects on the developing fetus are still not well elucidated, in which the perinatal exposure to morphine showed increased sensitivity to morphine reinforcement effect in the adult offspring. The present study investigated whether exposure to morphine during pregnancy and lactation can change nociception in adult offspring offspring of mothers treated with morphine. Pregnant female mice were exposed to morphine (10 mg / kg / day) subcutaneously for 42 days (21 days of pregnancy to 21 days of lactation). Upon completion 21 days, the progeny is sexed in males and females, then at 75 days of age, subjected to the open field test and nociception by the methods of writhing induced by acetic acid, hot plate and formalin. In the test of the total locomotion, the animals showed no motor changes. In nociception tests, we observed increased nociceptive response in male mice and female morphine group tested for contortion. Males who have been exposed perinatally to morphine decreased the nociceptive threshold in the second phase (inflammatory phase) of formalin. In the hot plate, the male and female animals showed abnormalities in pain sensitivity, reversing the sensitivity profiles of its controls, in which the group of males exposed perinatally to morphine showed increased sensitivity to thermal stimulus at 120 min test and the group of females exposed perinatally to morphine decreased thermal sensitivity when compared to their controls. These results suggest that exposure to morphine in the intrauterine period and lactation affects nociceptive thresholds in the offspring in later life and that this change is dependent on the type and length of exposure to noxious stimuli.
Acesso aberto (Open Access)
Petiveria alliacea L.: etnobotânica, fitoquímica efeitos neurofarmacológicos e cognitivos
(Universidade Federal do Pará, 2016-10-16) LUZ, Diandra Araújo da; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
Petiveria alliacea L. is a shrubby plant, native from tropic regions, used to treat poor memory and improve learning. In the present study, it was evaluated the effects of hydroalcoholic extract of the leaves of P. alliacea (PaLHE, 900 mg/kg) on learning and memory of adult rats, subjected to behavioral tests inhibitory avoidance and Morris Water Maze. In addition, it was performed a thin layer chromatography (TLC) to detect sulphur compound, to correlate them with the investigated responses. Dichloromethane fractions, obtained from aqueous extracts of Alllium sativum and Allium Cepa served as detection patterns. P. alliacea is also present controversial activities on central nervous system (CNS). For this reason, it was realized a bibliographic review of ethnobotanical, phytochemical and neuropharmacological activities of this plant in indexed databases, books, dissertations, thesis and similar scientific sources. According to the results, EHFPa improved short and long term memory, spatial memory and learning. In TLC, the EHFPa produced retention points similar to the standard samples, indicating that there are sulfur compounds in the extract, being possible that they contribute to observed responses. About the review, P. alliacea is popularly used to treat epilepsy, anxiety, poor memory, as a sedative, etc. Such properties have been demonstrated experimentally, varying depending on the dose, preparation, and part of plant used. Phytochemical studies detected several metabolites in P. alliacea, among which sulfur compounds, flavonoids and derivatives classes the most isolated compounds.
Acesso aberto (Open Access)
Uso da morfina no período gestacional e lactação: efeitos sobre o desenvolvimento do sistema nervoso central e comportamento
(Universidade Federal do Pará, 2013-09-17) SOUZA, Izabelle Camões de; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
The use of psychoactive substances is a major problem in modern society, causing social upheaval, economic and health of the user. In 2010, 26,4-36 million people used opioid analgesics for non-therapeutic purposes, and the frequency of alcohol abuse by health professionals, five times greater than the general population, due to easier access. The prevalence in use during pregnancy introduces rates ranging from 1% to 21%, accounting concern in society, since the effects caused on fetal development are not well understood. The aim of this study was to evaluate the neurobehavioral responses of adult rats after chronic exposure to morphine during the intrauterine period and lactation. Pregnant rats were given for 42 days subcutaneously, morphine 10 mg / kg / day. The offspring were weighed on days D1, D5, D10, D15, D20, D30, D60, and behavioral assays performed with the progeny to 2.5 months of age, which consisted of the open field model, elevated plus maze, forced swimming and rota-rod. The results demonstrated that exposure to morphine promote changes in the weight gain after the birth of offspring and even after weaning increase in spontaneous locomotion in females, as well as increased anxiogenic-like behavior and the behavior of the depressive type, regardless of sex, but without prejudice associated motor.