Navegando por Orientadores "RIBEIRO, Haroldo Francisco Lobato"

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Acesso aberto (Open Access)
Avaliação da dinâmica folicular, atividade ovariana e involução uterina durante o pós-parto de búfalas (Bubalus bubalis) criadas em condições tropicais
(Universidade Federal do Pará, 2015-08-11) SILVA, Aluizio Otavio Almeida da; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
In this experiment aimed to monitor the follicular dynamics and ovarian activity of buffalo females in the postpartum period through ultrasound exams, investigating whether some events related to ovarian activity in the Postpartum Period (PPP), and uterine involution, since the 7th day calved until the second post-partum estrus. ten were used (10) crossbred females between Murrah and Mediterranean breeds with eutócicas parities and no history of reproductive problems, managed in semi extensive system, with availability of water and mineral supplementation ad libitum. It was found the presence of follicular growth patterns of two waves (2 OCF) and three waves (3OCF) at a ratio of 40% and 60%, respectively. The 1st and 2nd postpartum estrus demonstrated to 37,00+13,12 and 58,83+14,56 days postpartum for 2OCF animals, 45,00 + 15,19 and 66,00 + 23.02 days postpartum for animal 3OCF. The estrous intervals were 21,00+2,44 and 21,83+2,56 days for animals 2OCF and 3OCF respectively. Follicular emergency occurred at an average of 1,02+0,34 and 7.25+0,50 days cycle for the first and second waveform, respectively, of animals and 2OCF 0,93+0,35; 6,6+1,36 and 10,83+1,94 days cycle for the first, second and third wave, respectively, for the animal 3OCF. The pre ovulatory follicular diameter was 13,08±2,6 and 11,45+0,35 mm for the 1st and 2nd postpartum estrus of 2 OCF animals and 13,24+2,41 and 12,74+1,10 mm for the 1st and 2nd postpartum estrus of 3OCF animals. The uterine involution occurred at an average of 29,00+1,48 days, (P <0.001), diameter of the post gravid uterine horn (DPGUH) around 18,84+0,33 mm. The rate of uterine involution was 0,97+0,21 mm/day, (P <0,001). There was a high correlation between the PPP and CUPG, represented by r = -0,8179 (P<0,0001), being possible to establish a linear regression between the two parameters, represented by the equation Y = 40,8809 + (-0,8214.X) (P<0,0001).
Acesso aberto (Open Access)
Eficiência reprodutiva em vacas mestiças leiteiras em municípios do nordeste paraense
(Universidade Federal do Pará, 2010-03-30) SIMÕES, André Reale; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
Cows reared in semi-intensive production systems of two farms, one in the city of Irituia and another in Mother River in northeastern Pará. We analyzed reproductive records collected during September 2006 to March 2009. We analyzed 851 females, 106 (12,45%) heifers and 745 (87,55%) cows with a total of 1,356 gynecological exams. The overall pregnancy rate was presented 87,74% to 63,35% for heifers and cows. In 1356 examinations of the animals had 66,41%, 33,59% are pregnant and not pregnant. The pregnancy rate in relation to the type of reproduction was 50,66%, 41,30% and 77,27% for insemination artificial conventional (IA), AI time-fixed (IATF) and mating natural (MN), respectively, with an average of semen doses per pregnancy of 2,01. The genetic groups Guzerá 87,03%, 71,74% and Gir 70,54% Brown Swiss showed differences (p <0,01) pregnancy rate compared to Girolando with 62,15% to 61,91% Simmental and Holstein with 59,45%. The pregnancy rate found in the dry season showed a 75,77% difference (p <0,01) obtained for the less rainy season with 58,95%. The mean age at first service (IPC) and the first calving (IPP) were 29,6 ± 5,71 and 38,57 ± 5,71 months respectively, occurring differences (p <0,05) in the IPP grating the type of reproduction IATF and MN and rainiest season and less rainy. The period of service (PS) was 110,97 ± 70,87 days, difference (p <0,05) between the rainiest season and less rainy, and also influences (p <0,05) the variable year study, decreasing from 2006 to 2008 respectively. The pathologies found were 2,35% ovary hypoplastic, cervix winding 1,52% 1,76% abortion, endometritis 1,29%, 0,7% Cervix fibrotic, follicular cyst 0,70%, 0,12% Adherence Tubovárica , cyst luteal 0,12%, Pneumovagina 0,12% and 0,12% Vulva child.
Acesso aberto (Open Access)
Polimorfismos na região promotora, Éxon I e Éxon II do gene do receptor da melatonina associados às características reprodutivas em búfalas na Amazônia
(Universidade Federal do Pará, 2015-06-18) BARBOSA, Elizabeth Machado; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
The buffaloes production in the Pará state have is a high representative activity to the regional economy and production of meat, milk and dairy products. Therefore, it is necessary to develop new technologies that increase the enjoyment of the production and reproduction of buffaloes in the Amazon region. Thus, the objectives of this research thesis was to characterize the promoter region of the melatonin receptor gene 1 (MTRN1A) and identify polymorphisms in exons I and II of that gene and link them to reproductive traits that have economic importance in buffaloes in the region Amazon. Were collected 400 buffaloes samples and 60 animals were used to characterize the promoter region, 140 to detect the polymorphism (SNPs) in exon II and 77 to detect the polymorphism in exon I. DNA extraction was peformed by phenol method. Then were selected different primers to make Polymerase Chain Reaction (PCR). The promoter region was sequenced MTRN1A a total of 1621 base pairs by the Sanger method, polymorphism in exon II was detected by PCR-RFLP (Restriction Fragment Length Polymorphism) with HpaI enzyme and the polymorphism exon I was found by sequencing DNA by Sanger method. Allele and genotype frequencies of all SNPs were evaluated, the inbreeding coefficient (Fis) and the likelihood of Hardy-Weinberg equilibrium. SNPs in the promoter region and exon I have been associated with reproductive traits of buffaloes by ANOVA or Student's t test and / or chisquare test. The significance level was 0,05. 26 SNPs were detected in the promoter region at positions -1511 (C → T), -1465 (G → T), -1422 (A → G), -1411 (G → A), -1395 (G → T), - 1298 (A → G) -1295 (G → A) -1242 (C → A) -1150 (C → T), -1 147 (G → C) -1136 (A → G) -911 ( G → A), -909 (A → G) -724 (C → G) -656 (A → C) -649 (C → T), -644 (G → A), -511 (A → C) -481 (G → A), -425 (C → T) -395 (G → A), -383 (G → T), -254 (C → T) -206 (T → C) , -133 (T → G) and -94 (C → T) and a deletion unit (ACAA) at position -1483. Of the total detected SNPs, 75% of the wild alleles tiveral frequencies greater than 0,5. For the characteristic interval between calving (IEP), only five SNPs (-1298, -1136, -911, -724 and - 656) were significantly associated (P <0.05) and three SNPs (-1395, -724 and -94) were significantly associated (P <0.05) with the characteristic age at first calving (IPP) and none for the characteristic concentration of deliveries (P> 0.05). A total of 11 SNPs was strongly associated with binding factors in gene regulation. The SNP in exon II by PCR-RFLP (HpaI) at position 306 (T → C), the most frequent allele T in Upland animals (0,529) and C in lowland animals, the two populations showed coefficients Fis inbreeding (0,040 and 0,091, respectively) and strong deviations from Hardy-Weinberg equilibrium (P> 0.05). The mutation occurs at codon 106 and is the sinonímio type without changing the messenger RNA handles structure. The SNP in exon I was detected at position 62 (T → C) and of the non sinonímio, exchanging of Leucine to Proline. The mutant allele was the most frequent C (0,623) inbreeding coefficient Fis = 0.397 and Hardy-Weinberg deviations (P <0.05). None of the genotypes were associated with calving interval and age at first birth (P> 0.05). Therefore, SNPs are strong candidates for selection, but it would be interesting to evaluate them in other herds in the Amazon region and / or in other regions of Brazil and / or other countries to effect them as excellent molecular markers for reproductive traits of buffalo.
Acesso aberto (Open Access)
Polimorfismos no gene da osteopontina e suas associações com a fertilidade de búfalos na Amazônia
(Universidade Federal do Pará, 2012-09-24) ROLIM FILHO, Sebastião Tavares; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
Because the osteopontin gene can influence the fertility of water buffaloes, the aim of this work was to identify polymorphisms in this gene and associate them with fertility parameters of animals kept under extensive grazing. We used 306 male buffaloes older than 18 months, bred on two farms, one in the state of Amapá and the other in the state of Pará. We identified three SNP for the region amplified by the primer OS4 (5`UTR) and four SNP polymorphisms for the region amplified by the primer OS9 (exon 5 to exon 6). The polymorphisms were in positions 1478, 1513 and 1611 in the region amplified by OS4 and positions 6690, 6737, 6925 and 6952 in the region amplified by OS9. We calculated correlations with the traits scrotal circumference and volume and sperm motility, concentration and pathology. There were SNPs for all the traits studied at 5% significance: for circumference, SNP 6690; for volume, SNP 6737; for concentration, SNP 6690; for motility, SNP 6690; and for pathology, SNP 6690. Therefore, SNP 6690 was related to four traits. The AA genotype of SNP 6690 presented the highest averages for scrotal circumference, sperm concentration and motility and the lowest total number of pathologies. However, for the scrotal volume trait, the animals with the largest volume were correlated with the presence of the GG genotype of SNP 6737. This indicates that the osteopontin gene is important because it can have a substantial influence on the reproductive traits of male buffaloes.
Acesso aberto (Open Access)
Prevalência de alterações clínicas e histopatológicas do sistema genital de búfalas (Bubalus bubalis) no Estado do Amapá
(Universidade Federal do Pará, 2007) MOURÃO, Fábio Rodrigo Paixão; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
Objecting to find out the prevalence of infertility diseases, 895 buffalo cows were submitted to clinical examination with main emphasis on the gynecological aspects and tuberculosis and brucellosis tests, located in Cutias do Araguari country, Amapá State, and analyzed 1000 genitals slaughtered buffalo cows. The prevalence of tuberculosis and brucellosis on clinical analysis were 9,33 and 9,11% respectively. On 895 clinical examined buffalos cows, 299 (33,41%) were pregnant, 188 (62,88%) on the right horn and 111 (37,12%) on left horn. On the buffalo cows group with some alteration, were discovered the following disorders: a) abnormal ovaries: non-functional ovaries, 75 (8,37%); follicular cysts, 6 (0,67%); b) oviduct abnormalities cases: slight adhesions, 24 (2,9%); severe adhesions, 22 (2,46%); hydrosalpinge, 8 (0,9%); c) uterine and cervical abnormalities cases: slight chronic endometrititis, 5 (0,56%); moderate chronic endometritis, 7 (0,78%); irregular cervix, 14 (2,9%); d) Vaginal and vulvar abnormalities cases: lacerated vulva, 9 (1,1%); totalizing 172 (19,21%) buffalo cows with some abnormalities. On the slaughtered buffalo cows were evidenced 661 (66,10%) of pregnancy buffalo cows, that 86 (8,6%) with some reproductive disorder and 339 (33,90%) not pregnancy buffalo cows with 74 (7,4%) with some reproductive disorder. In 661 pregnancy, 412 (62,3%) were in right horn and 249 (37,7%) on left horn. The reproductive abnormalities found were: a) ovarian abnormalities cases: non-functional ovaries, 16 (1,6%); para-ovarian cysts, 5 (0,5%), epithelial inclusion cysts, 8 (0,8%); follicular cysts, 1 (0,1%); b) oviductal abnormalities cases: hydrosalpinge, 18 (1,8%); tubo-ovarian cysts, 5 (0,5%); slight adhesions, 70 (7,0%); severe adhesions 21 (2,1%); c) uterine and cervical abnormalities: entometritis, 9 (0,9%); perimetritis, 3 (0,3%); adenomiosis, 1 (0,1%); segmental aplasia of uterus, 1 (0,1%); twining, 1 (0,1%); irregular cervix, 1 (0,1%). The major percentage of fetus were found on final third of gestation with 236 (35,65%) cases. Several are the problems that can affect the fertility of buffaloes cows and we need to identify it to solve the problems of productivity.
Acesso aberto (Open Access)
Prevalência de patologias no trato genital de machos bubalinos (Bubalus bubalis) nos estados do Pará, Amapá e baixo Amazonas, Brasil
(Universidade Federal do Pará, 2009-08-28) BARBOSA, Elizabeth Machado; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
Reproductive disorders are associated with problems acquired (stress, inadequate management and infectious origin), and genetic (high consanguinity exists in some herds in Brazil). While showing the degree of the male potential fertility, the breeding soundness examination and, in particular, the functionality test has been little used, particularly in bull selection, though; these same studies mention the semen importance to evaluate the male reproductive efficiency. The aim of this study was to determine the prevalence of diseases clinically notable, by palpation and inspection, and to characterize the profile of buffalo sperm created in systems production, intensive and extensive. The study was conducted on properties in the Pará State, in the Municipalities of Belém, Ipixuna, Mojú, Nova Timboteua, Paragominas, Santarém Novo and São Caetano de Odivelas, Muaná, Soure, Chaves and Ponta de Pedras. In Amapá State in the Municipalities of Cutias, Itaubal, Tartarugalzinho and Bailique Archipelago's. The animals were reared in an intensive and extensive, respectively. The period of study was from March 2008 to March 2009. 305 bulls were used Murrah, Mediterranean and mixed with ages ranging from 2 to 15 years, and 160 in the Pará State, and 145 in the Amapá State. Data from andrological clinical examinations, as well as semen analysis were recorded on special forms. Obtaining semen was performed by ampoules massage and the semen physical analysis was by the color, appearance, volume, motility, vigor, concentration, morphologic and pH. The concentration was measured by spectrophotometer on the Center of Animal Biotechnology (CEBRAN). For sperm morphology was used the Cerovsky method. 200 sperm were examined under a light microscope in immersion objective. For statistical analysis, we used the statistical program BioEstat 5.0, descriptive statistics for all variables studied: testicular biometry, disease of the reproductive system and the physical characteristics and morphology of semen, recording the means, standard deviations and frequency distribution for characteristics of andrology classes. Quantitative data concerning the age of bulls, testicular biometry, physical assessments and morphological characteristics of semen were submitted to ANOVA and when significant effects by F test, means were compared by Tukey test at 5% probability. Of the 305 buffalo, the beasts of the Amapá State and the average standard deviation of the scrotal circumference was higher than the Pará State independent of the type of management. Regardless of age and breeding systems found no change in the penis. The bifid scrotum (cleft scrotum), was the most prevalent. The highest frequency of change in epididymis. Tuberculous granulomatous epididymitis was observed in the extensive livestock in the Amapá State, with a tendency for bulls over four years. Among the changes in the testis was the most prevalent testicular torsion and infectious causes highlight the occurrence of chronic active interstitial orchitis, tuberculous granulomatous orchitis, testicular hypoplasia grade I, testicular hypoplasia grade III associated with tuberculous orchitis hypoplasia and grade II (partial). Another observation is quite clear regarding the percentage of spermatozoa with major and minor defects which have very high standards recommended by the CBRA. In this study we can conclude that breeders bred extensively present genetic diseases and infectious diseases are very prevalent.
Acesso aberto (Open Access)
Produtividade de vacas mestiças leiteiras em sistema semi-intensivo nos municípios de Irituia e Mãe do rio do nordeste paraense
(Universidade Federal do Pará, 2010-03-30) BARBAS, Cristiane de Carvalho; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
Check the influence of environmental factors on reproduction and production traits in dairy cattle genetic groups exploited in some farms in the municipalities of Irituia and Mother of the river in northeastern Pará. We used an average of 454 animals lactating crossbred Holstein, Brown Swiss and Girolando belonging two farms to system with the semi-intensive in the less rainy season. The mean and standard deviation for total milk production was equal to 1097,36 ± 330,47 kg, with a coefficient of variation equal to 25,64% and the group genetic Holstein, the less rainy season of birth and year of 2007 produced more. The period of lactation had an effect on the linear and increasing total milk production. The lactation period showed a mean and standard deviation equal to 218,17 ± 43,17 days, the highest average for the period of lactation was observed in the rainiest season and the average lactation period decreased over the years 2007 and 2008. The mean and standard deviation of calving interval found in the herd was equal to 398,975 ± 60,85 days. The least rainy season had an average calving interval of less than rainiest season and the average interval between births was reduced from 2006. The average age at first calving was found to be 38,57 ± 5,81 months. The coefficients of determination were greater than 0,85 (R2 a> 0,85), and genetic group for Holstein, the settings were better than those other two settings of genetic groups. For the genetic group Girolando, the shape of the lactation curve differed from the other two genetic groups difficult adjustment for the functions Gamma incomplete, Linear hyperbolic and Polynomial inverse. The adjustments made by the function Polynomial inverse showed slight deviation from the other two functions. The group presented genetic Holstein production peak and persistence slightly higher compared to other genetic groups.
Acesso aberto (Open Access)
Ultrassonografia testicular, em machos bubalinos criados em regime extensivo no estado do Pará
(Universidade Federal do Pará, 2011-07-07) MANRIQUE AYALA, Henry Daniel; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
The objective was to verify the biometrics associated with testicular ultrasound as a tool in the selection of sexual precocity in male buffalo. We used 19 male crossbred Murrah and Mediterranean breeds, aged between 11 and 59 months, from May 6 to November 18, 2010. The animals were divided into 12 age groups: 8 animals from 12 to 19 months, 3 animals from 20 to 29 months, and 8 animals from 30 to 59 months. The animals were kept under extensive system in the low land to pasture canarana (Eriochloa SP) without mineralization. The animals were subjected to clinical andrological examination with semen collection by transrectal massage of the ampoules and physical analysis of the ejaculate. The sonographic examinations were performed with the device model Mindray DP-2200, 75L50EAV transrectal linear transducer, multi-frequency 5.0 / 7.5 / 10.0 MHz echodensity The testicular (Ecot) was expressed in number of pixels / area, using the program Image J. We collected blood samples to measure testosterone levels. Statistical analysis was performed using the SAS statistical software 1999. The results of scrotal circumference show averages and standard deviations minimum and maximum of 12.88 ± 0.51cm for animals of 12 and 13 months and 28.38 ± 0.38cm in males aged greater than or equal to 60 months, respectively. The testicular volume measurements showed minimum and maximum values of 30.28 ± 17.37cm³ in animals of 12 and 13 months and 534.25 ± 25.36 cm³ in animals greater than or equal to 60 months, respectively. The mean and standard deviation of the sperm concentration maximum and minimum found were 5.5 ± 3.5 (x 10⁶ / mm³) for animals of 16 and 17 months and 51.41 ± 2.26 (x 10⁶ / mm³) for greater than or equal to 60 months , respectively. Sperm motility in the minimum and maximum percentages were obtained 10% for animals with 16 and 17 months and 80 ± 5.77% to greater than or equal to 60 months, respectively. The total sperm defects found were minimum and maximum of 18.5% in animals of 16 and 17 months and 3%. in greater than or equal to 60 months, respectively. Testosterone levels were found minimum and maximum of 0,070 ± 0,026 ng / ml for 12 and 13 months and 2762 ± 0457 ng / ml for animals greater than or equal to 60 months, respectively. The echogenicity for animals aged 12 to 13 months was 78.67 ± 6.36 pixels, in the range of 14 and 15 months was 94.22 ± 3.40 pixels, for animals aged 16 and 17 months was 88.16 ± 3.95 pixels, animals between 18 and 19 months was 96.09 ± 3.40 pixels, for animals of 20 and 21 months was 103.12 ± 3.86 pixels, for 22 and 23 months was 98.4 ± 5.87 pixels, for the age of 24 to 29 months was 114.05 ± 2.42 of pixels, aged 30 to 35 months was 109.24 ± 3.13 pixel for animals of 36 at 41 months was 98.67 ± 5.3 pixel, between 42 to 47 months the average was 99.33 ± 2.1 pixel, between 48 to 59 months was 96.17 ± 1.90 pixel and animals greater than or equal to 60 months of age was 90.13 ± 1.77 pixel. We conclude that ultrasonography, the interpretation of the echogenicity, associated with the clinical andrological examination, is a tool that can be used in the selection of precocity and fertility evaluation of male buffalo.
Acesso aberto (Open Access)
Viabilidade da dose fracionada de sêmen bovino criopreservado descongelada por diferentes métodos
(Universidade Federal do Pará, 2010-05-21) ARAUJO, Gilson Ferreira de; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
Given the low sperm concentration necessary to promote the fertilization of oocytes in FIV, this paper aims at evaluating the quality of dose of bovine semen cryopreserved after its splitting into two parts to make better use of it on the PIV program. The experiment used 110 doses of frozen semen in pallets of 0.25 mL from 10 bulls, which were divided into stages: A dose represented the control group, five were assigned to the group of direct thawing (DT) and 5 for the group of indirect thawing (IT). Doses were fractionated into two parts, forming two subgroups for each group, the unit sphere (UE) and polyvinyl alcohol unit (PAU). The first subgroup analysis was immediate, and the second was analyzed after 24h storage in N2. Results were obtained through descriptive statistics, Kruskal-Wallis and SNK (P <0.05), motility and force: immediate Indirect thawing: 48.8% / 2.4, IT-24: 54.2% / 2.5, immediate direct thawing: 67.8% / 3.0, direct thawing-24: 69.6% / 3.0, Control: 70% / 3.0. For acrosome injury and membrane integrity: immediate Indirect thawing: 24.3% / 40.5%, indirect thawing- 24: 36.2% / 44.3%, immediate direct thawing: 12.8% / 63.6%, DT -24h: 12.6% / 59.0%, control: 11.9% / 57.5%. Average sperm concentration: DI-immediate: 6.1 × 106 DI-24: 7.2 × 106, DD-immediate: 8.2 × 106, DD-24: 7.8 × 106 sperm / dose fraction. Thus for the fractionation of the dose of bovine semen cryopreserved best thawing method was straightforward, since it showed the best results in all parameters.