Navegando por Orientadores "SANTOS, Jeannie Nascimento dos"

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Acesso aberto (Open Access)
Análise taxonômica e molecular de Cestoda nematotaeniidae parasito de intestino delgado de Rhinella marina (Linnaeus, 1758) (Amphibia: Bufonidae) de Belém-Pa
(Universidade Federal do Pará, 2010-07-02) MELO, Francisco Tiago de Vasconcelos; SANTOS, Jeannie Nascimento dos; http://lattes.cnpq.br/4543897195525368
The amphibians of the species Rhinella marina known also as Giant Toad and have cosmopolitan distribution. Posses nocturnes habits, due of variety of feeding they can live in different habitats. Then, they can have many kinds of helminthes parasites. Among the helminthes, the cestodas are the target of study of this work. The members of Nematotaeniidae Family are commonly found in small intestine of repitilian and amphibians. The frequent auto-infestation would justify the high taxes of parasitism in one host. The present study has the objective to identify and to characterize the Cestoda of Rhinella marina from Belém-PA. Twenty hosts were captured in homes of the metropolitan area of Belém-PA and, after necropsy the Cestoda were isolated of intestine, and some specimens were fixed in A.F.A (Glacial Acid Acetic 2%, Formaldehyde 3% and 95 % of Etanol 70º GL), and some worms fixed in 2% Glutaraldehyde in Cacodilate buffer 0,1m P.h 7,4, to process in different techniques. One part of the samples was dehydrated in Etanol Series, and stained with Carmim®, and clarified with Metil Salicilate®. Some specimens were dehydrated and included in Paraffin for acomplishement of longitudinal and tranverse cuts. The worms fixed in Glutaraldehyde were dehydrated and included in Historesin®. Some Cestoda were processed for Scanning Electron Microscopy (SEM). An indentification was accomplished throught drawings in Olympus BX 41 microscope equipped with camera lucida, pictures were taken in a MEDILUX microscope, with image captures system and in MEV JEOL 5310. Histological sections were photographed and 3D reconstruction was made in RECONSTRUCTTM software. The cestoid possess a cylindrical body, filiform and with difficult segmentation, except in the final portion of the strobila. Escolex with four suckers without hooks or apical organ, the pregnant proglotis presents two piriform capsules, funded in the basis and containing one or more eggs. The observations in SEM and light microscopy of the cestoda founded in small intestine of R.marina from Belém-PA, we observed that these Cestoda belong to Nematotaeniidae Family, meanwhile the other morphologic characters observed did not permit us to classify this helminth in any Gender of this Family.
Acesso aberto (Open Access)
Taxonomia integrativa de nematódeos Oswaldocruzia (Trichostrongyloidea: Molineidae) da Amazônia Oriental
(Universidade Federal do Pará, 2019-04) COSTA, Yuri Willkens de Oliveira; SANTOS, Jeannie Nascimento dos; http://lattes.cnpq.br/4543897195525368; https://orcid.org/0000-0002-6612-6410
Oswaldocruzia is a genus of parasitic nematodes of amphibians (Anura, Caudata) and reptiles (Squamata), represented by about 90 species distributed worldwide, of which 43 occur in the Neotropical region. The species of Oswaldocruzia are mainly characterized by the spicular morphology of the males and are divided into five biogeographic groups (Oriental-Ethiopian, Neo-Ethiopian, Holartic, Neotropical Caribbean and Neotropical Continental) and also three morphological types of copulatory bursa (types I, II and III). However, morphological similarity, absence of updated identification keys and molecular data complicate the systematic of the genus. Thus, the objective of this study was the realization of the integrated taxonomy of nine species of Oswaldocruzia parasitizing eight species of amphibians and one reptile species from different collections in three locations in the state of Pará, Brazil. The hosts were necropsied and the helminths found were rinsed, fixed and stored in 70% ethanol. For the morphological study, the specimens were used for observation by light microscopy and scanning electron microscopy. For the molecular study, we performed the extraction, amplification and sequencing of the coding region of the Cytochrome c Oxidase Subunit I enzyme from the mitochondrial DNA. The resulting sequences were aligned and compared to ten sequences publicly available in GenBank and two phylogenetic reconstructions were performed to observe their relationships, one under the Maximum Likelihood criterion and the other by Bayesian Inference. As results we identified Oswaldocruzia belenensis in Rhinella marina and Rhinella margaritifera, Oswaldocruzia chabaudi in Boana geographica and Boana wavrini, Oswaldocruzia chambrieri in Amazophrynella bokermanni and R. margaritifera, Oswaldocruzia lanfrediae in Leptodactylus paraensis, Oswaldocruzia vitti in Anolis fuscuauratus, Oswaldocruzia sp. nov. 1 in Phyllomedusa vaillantii and Oswaldocruzia sp. nov. 2 in Osteocephalus oophagus. Alignment and comparison of levels of divergence demonstrated significant differences between the new obtained sequences and the sequences from GenBank. Both phylogenetic reconstructions demonstrated two main clades, one including the sequences from Mexico and another clade genetically distinct from the Amazon, highlighting the occurrence of O. chabaudi in B. wavrini and B. geographica, the close relationship between the sequences from parasites of Bufonidae and new records of hosts and localities for O. chambrieri and O. belenensis. This study adds information about the diversity of helminth parasites of amphibians and reptiles of Amazon, and shows that the combination of morphological and molecular methods presents satisfactory potential for species delimitation and characterization of the genus.