Navegando por Orientadores "TEIXEIRA, Francisco Martins"

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Avaliação da atividade dos extratos hidroetanólico de Chenopodium ambrosioides L. e de Eucalyptus alba Reinw ex Blume, frente a cepas de Mycobacterium sp
(Universidade Federal do Pará, 2014-10) VALÉRIO, Erika da Silva; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070; TEIXEIRA, Francisco Martins; http://lattes.cnpq.br/7648303522085382
Chenopodium ambrosioides and Eucalyptus alba are species used in folk medicine for the treatment of tuberculosis and sputum. This study aimed to determine the physical-chemical, microbiological parameters, define phytochemical screening and evaluate antimycobacterial, cytotoxicity, immunomodulation, and toxicity in vivo activities of extracts and fractions. In physical-chemical and microbiological evaluation of C.ambrosioides and E. alba extracts, were found parameters in accordance with the specific literature for medicinal plants. The phytochemical screening of the extracts revealed the presence of saponins, steroids, triterpenoids, phenols and tannins, C. ambrosioides extract also showed proteins and amino acids, while the E. alba extract was positive for organic acids and lactones. The results of broth microdilution assay and microplate alamar blue assay showed moderates activities against the Mycobacterium fortuitum of E. alba extract and ethyl acetate fraction (FAcE) of E. alba; the chloroform fraction (FCl) of C.ambrosioides and the ethyl acetate fraction (FacEA) of E. alba were moderately active against the Mycobacterium tuberculosis. The cytotoxic activity, evaluated by MTT method, showed that the extracts did not reduce cell viability in the concentrations tested. In the immunomodulation assay, E. alba extract presented potential anti-inflammatory effect, by the methods of inhibition the production of NO and TNFα. No signs of acute oral toxicity of the extracts at a dose of 2500 mg/kg in mice were detected. These results suggest the potential antimycobacterial the FAcE of E. alba and immunomodulatory of E. alba extract and can serve as a resource for future studies, aimed at isolation of active compound and elucidation of their mechanisms of action.
Desconhecido
Extratos de Pyrostegia venusta: caracterização físico-química, capacidade imunomoduladora e prevenção da formação do biofilme dental
(Universidade Federal do Pará, 2013-09-27) SOUSA, Mayara Brito de; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994; TEIXEIRA, Francisco Martins; http://lattes.cnpq.br/7648303522085382
Pyrostegia venusta is a climbing plant popularly known as “flor-de-São-João”. In traditional medicine is used to treat various diseases. Within the scientific literature various bioactive compounds have been isolated and some important biological activities as antimicrobial e immunomodulatory activities have been attributed to this specie. Caries and periodontal disease are the most prevalent oral pathologies among Brazilian population and dental biofilm is the main etiological factor of these diseases. Oral candidiasis is an infection caused by species of the yeast Candida which in some cases may behave as opportunistic pathogens. The aim of this study was to evaluate the physical, chemical and biological aspects of Pyrostegia venusta and its potential in the prevention of the main oral diseases. It was evaluated the cytotoxicity of the crude extract and its fractions in peripheral blood mononuclear cells and murine macrophages. The immunomodulatory activity of the crude extract and its fractions was verified through the dosage of nitric oxide (NO) production by macrophages murines stimulated with lipopolysaccharide (LPS). Through broth microdilution assay it was determined the minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and minimal fungicidal concentration (MFC) of the crude extract, fractions and isolated compounds. It was also evaluated the inhibition of Streptococcus mutans adherence to a glass cover slip, pH reduction capacity and the effect on germ-tube formation and budding of Candida albicans of the crude extract and its fractions. Pyrostegia venusta was not cytotoxic at low concentrations. NO production was inhibited by ethyl acetate and n-butanol fractions. Crude extract, fractions and isolated compounds have antimicrobial activity against Streptococcus mutans, Streptococcus mitis, Streptococcus oralis and Candida albicans; they also inhibited the adherence and were able to reduce the pH. Crude extract and hexane, ethyl acetate and n-butanol fractions inhibited the germ-tube formation and budding of Candida albicans. Pyrostegia venusta can be an alternative in prevention and treatment of the main oral diseases. However, other studies are necessary before it is considered as a promising specie.