Artigos Científicos - FCB/CALTA

URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/5323

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Efeito da escarificação e luminosidade na germinação in vitro de sementes de cagaiteira (Eugenia dysenterica DC.)
(2007-12) MARTINOTTO, Cristiano; PAIVA, Renato; SANTOS, Breno Régis; SOARES, Fernanda Pereira; HERRERA, Raírys Cravo; SILVA, Álvaro Augusto Naves
Cagaiteira is a native specie of brazilian cerrado with high yield. Because it seeds show high variability, it is yet considered recalcitrant and dormant, the germination in vitro appears as an alternative for specie propagation, in addition to obtain juvenile explant for micropropagation. The present research aimed to evaluate the effect of scarification and ligth on germination in vitro of cagaiteira seeds. Seeds without teguments and intact seeds were inoculated in MS medium and kept in abscence and presence of radiation. Seeds without tegument germinated in the presence or abscence of ligth showed 86.25% and 88.25% germination at 31 and 71 days of inoculation, respectively. After 150 days of inoculation, plantlets from seeds without tegument germinated in the presence and abscence of ligth, showed 10% and 12% of abnormalities, respectively.
Acesso aberto (Open Access)
Efeito de meios de cultura, concentrações de GA3 e pH sobre a germinação in vitro de mangabeira (Hancornia speciosa Gomes)
(2009) SOARES, Fernanda Pereira; PAIVA, Renato; STEIN, Vanessa Cristina; NERY, Fernanda Carlota; HERRERA, Raírys Cravo; OLIVEIRA, Lenaldo Muniz de
The Hancornia speciosa Gomes species presents potential for fruit and rubber production. Propagation is difficult primarily due, to a reduced seed germination and occurrence of recalcitrant seeds that stimulate the search of rapid and efficient propagation alternatives. In this context, the aim of this work was to study in vitro seed germination conditions in order to produce explants to be used on in vitro culture. The effect of different culture media, sucrose and GA3 concentrations and three pH levels were evaluated. Seeds were extracted from mature fruits after being harvested and processed. Higher in vitro germination was obtained using WPM and MS/2 media supplemented with 15.0 g L-1, 0.2 mg L-1 GA3 and pH adjusted to 5.8.
Acesso aberto (Open Access)
Indução, análises morfológicas e ultraestruturais de calos de maracujazeiro nativo
(Universidade Federal do Pará, 2015-08) CARVALHO, Milene Alves de Figueiredo; PAIVA, Renato; HERRERA, Raírys Cravo; ALVES, Eduardo; CASTRO, Evaristo Mauro de; PAIVA, Patrícia Duarte de Oliveira; VARGAS, Daiane Peixoto
Analysis of calluses with embryogenic characteristics is important for subsequent in vitro regeneration of species with desirable agronomic traits such as the Passiflora gibertii native passion fruit. In this context, the present study aimed to analyze the induction of calluses from leaf explants of Passiflora gibertii N. E. Brown, as well as their morphologic and ultra-structural characterization. Cotyledonary leaves were inoculated in culture medium containing 2,4-D and picloram in association with kinetin. After 30 days of cultivation in the dark, the obtained calluses were prepared for the visualization in electron microscope (transmission and scanning) and light microscope. The results allowed to infer that the addition of picloram and kinetin to the culture medium promotes higher callus formation in cotyledonary leaf explants of P. gibertii than 2,4-D and kinetin. The growth regulator 2,4-D provides the formation of calluses with small isodiametric cells and small intercellular spaces, an organized cellular system and the predominance of round shaped mitochondria. Predominance of large and elongated cells with intercellular spaces, a non-organized cellular system and predominance of elongated mitochondria was observed when picloram was used.
Acesso aberto (Open Access)
Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
(2014-12) CARVALHO, Milene Alves de Figueiredo; PAIVA, Renato; STEIN, Vanessa Cristina; HERRERA, Raírys Cravo; PORTO, Jorge Marcelo Padovani; VARGAS, Daiane Peixoto; ALVES, Eduardo
This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.

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