Navegando por Autor "ARAÚJO, Marialva Tereza Ferreira de"

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Acesso aberto (Open Access)
Association of killer cell immunoglobulin-like receptor polymorphisms with chronic hepatitis C and responses to therapy in Brazil
(2013) VASCONCELOS, Janaina Mota de; MÓIA, Lizomar de Jesus Maués Pereira; AMARAL, Ivanete do Socorro Abraçado; MIRANDA, Esther Castello Branco Mello; TAKESHITA, Louise Yukari; OLIVEIRA, Layanna Freitas de; MENDES, Lilian de Araújo Melo; SASTRE, Danuta; TAMEGÃO-LOPES, Bruna Pedroso; PEDROZA, Larysse Santa Rosa de Aquino; SANTOS, Sidney Emanuel Batista dos; SOARES, Manoel do Carmo Pereira; ARAÚJO, Marialva Tereza Ferreira de; BANDEIRA, Camila Lucas; SILVA, Adriana Maria Paixão de Sousa da; MEDEIROS, Zilene Lameira de; SENA, Leonardo dos Santos; DEMACHKI, Sâmia; SANTOS, Eduardo José Melo dos
Soroprevalence for Hepatitis C virus is reported as 2.12% in Northern Brazil, with about 50% of the patients exhibiting a sustained virological response (SVR). Aiming to associate polymorphisms in Killer Cell Immunoglobulin-like Receptors (KIR) with chronic hepatitis C and therapy responses we investigated 125 chronic patients and 345 controls. Additionally, 48 ancestry markers were genotyped to control for population stratification. The frequency of the KIR2DL2 and KIR2DL2+HLA-CAsp80 gene and ligand was higher in chronic infected patients than in controls (p < 0.0009, OR = 3.4; p = 0.001, OR = 3.45). In fact, KIR2DL3 is a weaker inhibitor of NK activity than KIR2DL2, which could explain the association of KIR2DL2 with chronic infection. Moreover, KIR2DS2 and KIR2DS2+HLA-CAsp80 (p < 0.0001, OR = 2.51; p = 0.0084, OR = 2.62) and KIR2DS3 (p < 0.0001; OR = 2.57) were associated with chronic infection, independently from KIR2DL2. No differences in ancestry composition were observed between control and patients, even with respect to therapy response groups. The allelic profile KIR2DL2/KIR2DS2/KIR2DS3 was associated with the chronic hepatitis C (p < 0.0001; OR = 3). Furthermore, the patients also showed a higher mean number of activating genes and a lower frequency of the homozygous AA profile, which is likely secondary to the association with non-AA and/or activating genes. In addition, the KIR2DS5 allele was associated with SVR (p = 0.0261; OR = 0.184).The ancestry analysis of samples ruled out any effects of population substructuring and did not evidence interethnic differences in therapy response, as suggested in previous studies.
Acesso aberto (Open Access)
Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil
(2005-12) MARTINS, Luisa Caricio; CORVELO, Tereza Cristina de Oliveira; DEMACHKI, Sâmia; ARAÚJO, Marialva Tereza Ferreira de; ASSUMPÇÃO, Mônica Baraúna de; VILAR, Simone Cristina Araujo Jucá; FREITAS, Felipe Bonfim; BARBOSA, Hivana Patricia Melo; FECURY, Amanda Alves; AMARAL, Renata Kelly Costa do; SANTOS, Sidney Emanuel Batista dos
We have examined the prevalence of gene cagA and vacA alleles in 129 patients, 69 with gastritis and 60 with peptic ulcer diseases from North Brazil and their relation with histopathological data. vacA and cagA genotype were determined by polymerase chain reaction. Hematoxylin-eosin staining was used for histological diagnosis. 96.6% of the patients were colonized by Helicobacter pylori strains harboring single vacA genotype (nont-mixed infection). Among them, 11.8% had subtype s1a, 67.8% had subtype s1b, and 17% subtype s2. In regard to the middle region analysis, m1 alleles were found in 75.4% and m2 in 21.2% of patients. The cagA gene was detected in 78% patients infected with H. pylori and was associated with the s1-m1 vacA genotype. The H. pylori strains, vacA s1b m1/cagA-positive, were associated with increased risk of peptic ulcer disease and higher amounts of lymphocytic and neutrophilic infiltrates and the presence of intestinal metaplasia. These findings show that cagA and vacA genotyping may have clinical relevance in Brazil.
Acesso aberto (Open Access)
Evaluation of the therapeutic response of hepatitis C in coinfected patients (HIV/HCV): a study of cases from a hospital for chronic liver diseases in the Eastern Brazilian Amazon
(2013-02) AMARAL, Ivanete do Socorro Abraçado; MÓIA, Lizomar de Jesus Maués Pereira; BARBOSA, Maria Silvia de Brito; DEMACHKI, Samia; ARAÚJO, Marialva Tereza Ferreira de; SOARES, Manoel do Carmo Pereira
INTRODUCTION: The aim of this study was to evaluate the therapeutic response of hepatitis C in patients coinfected with human immunodeficiency virus (HIV-1). METHODS: A retrospective study of 20 patients coinfected with HIV-1/HCV who were treated in the outpatient liver clinic at the Sacred House of Mercy Foundation Hospital of Pará (Fundação Santa Casa de Misericórdia do Pará - FSCMPA) from April 2004 to June 2009. Patients were treated with 180µg PEG interferon-α2a in combination with ribavirin (1,000 to 1,250mg/day) for 48 weeks. The end point was the sustained virological response (SVR) rate (HCV RNA negative 24 weeks after completing treatment). RESULTS: The mean age of the patients was 40±9.5 years, of which 89% (n=17) were male, and the HCV genotypes were genotype 1 (55%, n=11/20), genotype 2 (10%, n=2/20) and genotype 3 (35%, n=7/20). The mean CD4+ lymphocyte count was 507.8, and the liver fibrosis stages were (METAVIR) F1 (25%), F2 (55%), F3 (10%) and F4 (10%). The early virological response (EVR) was 60%, the end-of-treatment virological response (EOTVR) was 45% and the SVR was 45%. CONCLUSIONS: The median HCV viral load was high, and in 85% of cases in which highly active antiretroviral therapy (HAART) was used, none of the patients with F3-F4 fibrosis responded to treatment. Of the twenty patients treated, 45% achieved SVR and 45% achieved EOTVR. Studies that include cases from a wider region are needed to better evaluate these findings.
Acesso aberto (Open Access)
Expressão dos antígenos ABH e Lewis na gastrite crônica e alterações pré- neoplásica da mucosa gástrica
(2002-12) AGUIAR, Délia Cristina Figueira; CORVELO, Tereza Cristina de Oliveira; ARAÚJO, Marialva Tereza Ferreira de; CRUZ, Ermelinda do Rosário Moutinho da; DAIBES, Samiry; ASSUMPÇÃO, Mônica Baraúna de
BACKGROUND:The major cause for chronic gastritis in human is the infection by the Helicobacter pylori. The blood group antigens present at the gastric mucous are described as possible receptor for this bacteria in the epithelium. The alterations in the expression of blood group patterns are associated with the development of gastric cancer. OBJECTIVES: Verify the H. pylori prevalence and examine the immunohistochemical distribution of the ABH and Lewis antigens expression to correlate with histopathological alterations. PATIENTS AND METHODS: From 63 chronic gastritis patients were investigated gastric biopsies, blood and saliva samples by dot-blot-ELISA, indirect immunoperoxidase and hematoxylin-eosin and Gram. RESULTS: No significant association between the presence of the bacteria and the ABH, Lewis and Secretor phenotype was found. For the majority of the patients the antigen expression of the ABH and Lewis blood group was restricted mainly to the foveola epithelium of the gastric mucosa, similar to the saliva. The inappropriate expression of these antigens occurred always in the presence of H. pylori and/or preneoplastic alterations of the gastric mucosa. In areas with intestinal metaplasias we also observed reduced reactivity for the H and Leb antigens and mainly the induced expression of Lea. CONCLUSION: Alterations in the pattern of the glycosylation of this antigens are interesting, because they reflect different stages in the cellular differentiation and become potential markers in the diagnostic evaluation and prognosis of gastric pathologies.
Acesso aberto (Open Access)
Immediate graft histological assay, post pig's liver transplantation
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-09) BRITO, Marcus Vinicius Henriques; ARAÚJO, Marialva Tereza Ferreira de; CUNHA, Luane Cristine Batista; OLIVEIRA, Antônio Jorge Barbosa de; RODRIGUES, Jacqueline Barreto; BRITO, Nathalya Botelho
PURPOSE: To describe the vascular and tissue histopathological changes in seven sequential experimental liver transplantations in pigs. METHODS: Fourteen female pigs, Sus domesticus species, with body mass between 5 and 8 kg were utilized. After the end of all anastomoses of the graft implantation in the receptor, the animal was monitored for 30 minutes, and at its end one of the biopsies was collected for histological analysis. The histological criteria utilized were: lytic hepatocyte necrosis, density of septal and portal inflammatory infiltrated, sinusoidal congestion and hemorrhage. The analysis was performed separately for the portal region in zone 1, 2 and 3. RESULTS: Among the structural changes undergone by the graft, those with greater frequency and intensity were vascular congestion and steatosis, which stood out in transplantations 5, 6 and 7. CONCLUSIONS: The technique demonstrated vascular alterations represented by vasocongestion, edema and minimum inflammatory reaction. In relation to the parenchyma, was observed macrovacuolar pan-acinar steatosis, focal lytic and occasional hemorrhages, beyond the accumulation of hemosiderin in Kuppfer's cells.
Acesso aberto (Open Access)
Infecções pelos vírus das hepatites B e C e o carcinoma hepatocelular na Amazônia oriental
(2004) MIRANDA, Esther Castello Branco Mello; MÓIA, Lizomar de Jesus Maués Pereira; AMARAL, Ivanete do Socorro Abraçado; BARBOSA, Maria Silvia de Brito; CONDE, Simone Regina Souza da Silva; ARAÚJO, Marialva Tereza Ferreira de; CRUZ, Ermelinda do Rosário Moutinho da; DEMACHKI, Samia; BENSABATH, Gilberta; SOARES, Manoel do Carmo Pereira
In order to contribute to a better understanding of the possible role of hepatits B and C in the etiopathogenis of HCC in the East Amazon, there were studied 36 patients in Belém/PA. Serological hepatitis markers were evaluated and polymerase chain reaction assays were used to detect HBV-DNA and HCV-RNA. Alcohol abuse was observed in 33.3% and cirrhosis in 83.3%. In 88.9% of the sample, one or more hepatitis B markers were positive. Also, 8.3% those patients had anti-HCV simultaneously positive. The HBsAg serological test was positive in 58.3%; anti-HBc in 86%; anti-HBe in 85.7%; anti-HBe in 9.5%; IgM anti-HBc in 57.1%. The HBV DNA was found in 37.7% and in 65% of the HBsAg positive. The HCV RNA was detected in 8.5% and in 100% of' the patients positive to anti-HCV. The AFP was above the normal value in 88.9% of patients, with levels up to 400ng/ml in 75% of them. In conclusion, hepatitis B virus infection seems to be important in the etiology of HCC and improving measures such immunization and screening in the risk population should be emphasyzed.
Acesso aberto (Open Access)
Lesão intestinal após isquemia-reperfusão: estudo comparativo usando sal tetrazólico (MTT) e histologia
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2001-03) BRITO, Marcus Vinicius Henriques; ARAÚJO, Marialva Tereza Ferreira de; ACÁCIO, Glayce June Sasaki; ACÁCIO, Glayce Josy Sasaki; REIS, José Maciel Caldas dos
Many methods are used to evaluate ischemia reperfusion injury, but the most employed one is the histological study. However, it only demonstrates on mucosal tunic, the index of lesion and morphologic preserved cells, but not the index of viable functional cells, present in the sample. With this purpose, a colorimetric method was used, employing Methyl Thiazolyl Blue (MTT). The experiment was conducted in 30 Wistar male rats, distributed in 3 groups: Group Control (GC), Group ischemia and reperfusion-1 (GIR-1) and Group ischemia and reperfusion-3 (GIR-3), with 10 animals each. The Groups GIR-1 and GIR-3 were submitted to intestinal ischemia for 30 minutes by a false ligature of superior mesenteric artery, and submitted to euthanasia after 1 and 3 days of reperfusion, when material was picked for absorbency and histological procedures. It was observed a smaller proportion of viable cells and a larger degree of mucosal lesion in GIR-3 group (p<0.05), while GC group was the one with the larger proportion of viable cells and smaller degree of the mucosal injury (p<0.05). This way we concluded that MTT is as effective and reliable as the histological study at evaluating alterations produced by intestinal ischemia-reperfusion.
Acesso aberto (Open Access)
Prevalência de genótipos e de mutantes pré-core A-1896 do vírus da hepatite B e suas implicações na hepatite crônica, em uma população da Amazônia oriental
(2004) CONDE, Simone Regina Souza da Silva; MÓIA, Lizomar de Jesus Maués Pereira; BARBOSA, Maria Silvia de Brito; AMARAL, Ivanete do Socorro Abraçado; MIRANDA, Esther Castello Branco Mello; SOARES, Manoel do Carmo Pereira; BRITO, Elizabete Maria de Figueiredo; SOUZA, Olglaíze do Socorro Costa; ARAÚJO, Marialva Tereza Ferreira de; DEMACHKI, Samia; REBELLO, João Renato Pinho; MESQUITA, Michele Gomes Soares; BERTOLLINI, Denis Alberto; ISHAK, Ricardo
Hepatitis B virus (HBV) infection presents itself with a variety of clinical manifestations. The present work aims to describe the prevalence of HBV genotypes and the occurrence of precore mutation A-1896 in a population group of the Eastern Amazon region of Brazil and to correlate them with the clinical presentation of chronic HBV infection. 51 HBsAg carriers (HBV-DNA positive) were selected and divided into three groups: A (14 asymptomatic subjects), B (20 HBeAg positive symptomatic patients) and C (17 HBeAg negative symptomatic patients). Using an automa ed DNA sequencer ABI model 377 by sequencing for determined of genotypes and precore mutation. The results showed that the genotype A was the most commonly found (81,1%, 89,5% and 93,7% in groups A, B and C, respectively) and precore mutation A-1896 was described in 11,5% (3/26) of group A subjects. Genotype A of HBV was the most prevalent (89,1%) and low occurrence of precore mutation A-1896, both not associate with the worst outcome of the chronic infection of HBV.
Acesso aberto (Open Access)
Programa de hepatopatias do Hospital da Fundação Santa Casa de Misericórdia do Pará: infra-estrutura e epidemiologia clínica em 10 anos de atendimento
(2004) MÓIA, Lizomar de Jesus Maués Pereira; AMARAL, Ivanete do Socorro Abraçado; CONDE, Simone Regina Souza da Silva; BARBOSA, Maria Silvia de Brito; MIRANDA, Esther Castello Branco Mello; MACEDO, Zilvana Pinheiro de; ARAÚJO, Marialva Tereza Ferreira de; DEMACHKI, Samia; SOARES, Manoel do Carmo Pereira
The Liver Diseases Program of the Hospital Santa Casa de Misericordia do Pará was create because of the need to attend patients with liver diseases of the Amazônia area, taking as priority to attend with quality, diagnosis of aetiologies, clinical following and specific treatment. This study aim to describe dates related to epidemiology, aetiologics agents and histopathologic analisys. One thousand sixthy nine patients were evaluated through medical, laboratory, endoscopic, ultrasound or computadorized tomography and histopathologic examination. Nine hundred thirty five (63,6%) patients within 1469 patients were diagnose as chronic liver disease. The average age was 50 year, 666 (71,2%) were male, and the most patients lived in Belem, state of Pará. The aetiologic agents most prevail were alcoholism (53,7%) and viral hepatitis (39,1%). Hepatic biopsy were done in 403 (43,1%) within the 935 patients and the results showed chronic hepatitis (34%) and chirrosis (34%). In sumary the chronic liver disease in the amazon region is more prevail in male than female, the alcoholism is the principal aetiologie, and the most of these cases were diagnose in the severe phase.