Navegando por Autor "BARBOSA, Sandro"

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Acesso aberto (Open Access)
Decontaminant solution on in vitro growth of Byrsonima intermedia seedlings
(2015-04) SILVA, Luciano Coutinho; PAIVA, Renato; VARGAS, Daiane Peixoto; SILVA, Diogo Pedrosa Corrêa da; BARBOSA, Sandro; HERRERA, Raírys Cravo
Byrsonima intermedia A. Juss., is a medicinal and fruit plant of the Cerrado in which the conventional propagation is difficult due to the presence of extremely lignified endocarps. Although sodium hypochlorite (NaOCl) is widely used in the surface decontamination, there are few reports of its effects on explant growth. The aim of this work was to study the effect of different pH and exposure periods of B. intermedia seeds to a NaOCl solution. Seeds were subjected to different exposure periods (1, 5 and 10 minutes) to a NaOCl solution at different pH (5, 7, 10 and 12) and after treatment with NaOCl, embryos were inoculated in a WPM medium with 50% concentration of salts without sucrose, 0.5% agar and pH 5.8 and after 75 days of culture the growth of seedlings was evaluated. The use of NaOCl is effective in the decontamination of B. intermedia seeds, independent of pH variation and exposure periods and the parameters such as percentage of normal seedlings, shoot length, and number of leaves are positively affected by the use of NaOCl solution at pH 8.5-8.9 and by increasing the exposure period, however, the number of roots is affected only by increasing the exposure period in the NaOCl solution.
Acesso aberto (Open Access)
Métodos para a superação da dormência fisiológica de Caryocar brasiliense Camb.
(2010-06) DOMBROSKI, Jeferson Luiz Dallabona; PAIVA, Renato; ALVES, João Mauricio Cavalcante; SANTOS, Breno Régis; NOGUEIRA, Rairys Cravo; PAIVA, Patrícia Duarte de Oliveira; BARBOSA, Sandro
Caryocar brasiliense is a native species from Brazilian cerrado that has a considerable economic potential. The aim of this work was verifying the effect of substances with potential for seed germination stimulation. Seeds were extracted from the endocarps and set to germinate in paper rolls moistened with the following solutions: distilled water (Control); 2mmol L-1 KNO3 (Nitrate); 2mmol L-1 Etephon (ET); 1mmol L-1 GA3 (GA), 1mmol L-1 GA3 + 2mmol L-1 Etephon (GA + ET). The germination and the germination mean time were, respectively, 54.0% and 9.3 days in the GA treatment, 47.3% and 11.0 days in the GA + ET treatment, 32.0% and 12.2 days in the Control treatment, 30.7% and 13.1 days in the Etephon treatment, and 20.1% and 13.1 days in the Nitrate treatment. Low germination rates were observed in the GA + ET treatment up to the ninth day from sowing, when compared to the GA treatment, what indicates a possible seed germination inhibition due to ethylene presence.