Navegando por Autor "BRITO, Adriel Behn de"

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Acesso aberto (Open Access)
Expressão gênica e viabilidade de folículos pré-antrais inclusos em fragmentos de córtex ovarianos cultivados in vitro de macaco-prego (Sapajus apella)
(Universidade Federal do Pará, 2011) BRITO, Adriel Behn de; SANTOS, Regiane Rodrigues dos; http://lattes.cnpq.br/0500967766886604; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149
The aim of the present study was to investigate the stability of three reference genes in the ovarian tissue of capuchin monkeys (Sapajus apella) and to develop a short-term in vitro culture system for the activation and growth of preantral follicles from capuchin monkeys. To this end two experiment were conducted as follow. Experiment I: Fresh and cryoprotectant exposed ovarian biopsies were used. Both fresh and exposed ovarian tissues were subjected to total RNA extraction and synthesis of cDNA. After amplification of cDNA by real-time PCR, the GeNorm, Bestkeeper and Normfinder software were used to evaluate the stability of glyceraldehyde-2-phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyltransferase 1 (HPRT1) and TATA-binding protein (TBP). Experiment II: Ovarian tissue from four healthy mature females were collected and divided into nine ovarian cortical pieces of 1 mm³. One ovarian fragment (control) was immediately divided in two pieces, which were subjected to viability analysis or qRT-PCR. The remaining 8 fragments were individually cultured in vitro in a medium consisting of TCM supplemented with 100 ng/mL EGF (T1), either or not added with 10 μM BME (T2), 100 ng/mL BMP4 (T3), 25 IU PMSG (T4), 10 μM BME and 100 ng/mL BMP4 (T5), 10 μM BME, 25 IU PMSG (T6), 100 ng/mL BMP4, 25 IU PMSG (T7) or 10 μM BME, 100 ng/mL BMP4, 25 IU PMSG (T8). Results demonstrated that, in the ovarian tissue from capuchin monkeys, HPRT1 and TBP were the most suitable reference genes and thus could be used as parameters to normalize data in future studies. In contrast, GAPDH appeared as the least stable gene among the tested reference genes. After in vitro culture, all treatments resulted in similar percentages of viable preantral follicles. Ovarian tissue cultured in the presence of EGF + BME/BMP4/PMSG resulted in an increased rate of follicular activation and growth, as well as in the up regulation of AMH, BMP15 and GDF9, specific markers of follicular development. In conclusion, HPRT1 and TBP are the most stable reference genes in fresh and cryoprotectant exposed ovarian tissue from capuchin monkeys. Preantral follicles are able to develop in vitro when cultured in a medium supplemented with PMSG, BME and BMP4. Follicular viability, however, was maintained independently on the culture medium. The use of growth factors as markers of follicular development was crucial to identify the best culture medium.
Acesso aberto (Open Access)
Indução do estro em cutias (Dasyprocta leporina) utilizando-se protocolos à base de prostaglandina isolada ou em associação com análogo de GnRH
(FEPMVZ Editora, 2018-06) PEIXOTO, Gislayne Christianne Xavier; MAIA, Keilla Moreira; CAMPOS, Lívia Batista; OLIVEIRA, Gislaine Borba; OLIVEIRA, Maurilio Fernandes de; BRITO, Adriel Behn de; DOMINGUES, Sheyla Farhayldes Souza; SILVA, Alexandre Rodrigues; ALMEIDA, Laressa Marques de
We compared the efficiency of protocols for estrus induction in agoutis. Five females received double intraperitoneal administration of cloprostenol (5µg) on a 2-days interval; other five females were treated with intravulvar administration of 30µg gonadotrophin release hormone analogue (GnRH associated to intraperitoneal administration of 5µg cloprostenol after seven days and a new administration of GnRH analogue after two days. Every 3 days, the agoutis’ reproductive cycle was monitored by blood collection for hormonal analysis, ovarian ultrasound and vaginal cytology. Two females, originally in luteal phase, that received isolated prostaglandin presented estrous signs at 3 and 6 days after the second drug administration. From the females that received the association, two that were originally in luteal phase presented estrus at 4 days after treatment, and one other presented estrus only after 10 days. There was no significant statistical difference regarding the efficiency of treatments for estrus induction (P>0.05). We conclude that, according to the protocols tested in the study, the use of isolated prostaglandin or its association to GnRH analogue for estrus induction in D. leporine shows an efficiency limited to the females that were in luteal phase in the beginning of the treatment.
Acesso aberto (Open Access)
Relação entre a biometria ultra-sonográfica em modo B do bulbo ocular e os diâmetros fronto occiptal e bizigomático em Canis familiaris
(2009-04) CARVALHO, Poliana Silva Beserra; SALES, Gustavo Aguiar; SANTANA, Expedito Junior de Matos; MIRANDA, Stefânia Araújo; BRITO, Adriel Behn de; NIKOLAK, Elizabete; DOMINGUES, Sheyla Farhayldes Souza
In the experiment 31 healthy mongrel dogs, 8 months to 7 years of age, were used, 10 males and 21 females, weighing 1.5-28 kg. Initially, fronto-occipetal (FOD) and bizigomatic (BZD) diameters were measured using a caliper. The ophthalmologic transpalpebral B-mode ultrasonography (US) was performed to measure the ocular bulbi structures, as follows: The cornea thickness (D1), distance between cornea and anterior lens capsule (D2), distance between cornea and posterior lens capsule (D3), lens thickness (D4), lens diameter (D5), lens area (D6), distance between posterior lens capsule and retina (D7), distance between anterior lens capsule and retina (D8), and the distance between cornea and retina (D9). Except for D4, there were effect of FOD and BZD on the measures of the internal structures of BO. The Lineal Regression Analysis between the measures of the internal oculars structures and DFO and DBZ were significant for D1, D2, D3, D4, D5, D6, D7, D8 and D9.
Acesso aberto (Open Access)
Triplex Doppler da artéria renal e a relação entre a ecobiometria dos rins com distância atlanto-coccígea e altura em Canis familiaris
(2009-10) SANTANA, Expedito Junior de Matos; CARVALHO, Poliana Silva Beserra; BRITO, Adriel Behn de; MIRANDA, Stefânia Araújo; NIKOLAK, Elizabete; DOMINGUES, Sheyla Farhayldes Souza
The aim of this paper was to establish the relation between the kidney ecobiometry with atlanto-coccyges distance (ACD) and height (H) in adult healthy dogs, to obtain normality parameters for assessment of renal size and volume, as well as establish reference values to evaluate kidney blood perfusion by the resistivity index (RI) and pulsatility index (PI) of main renal artery. The study was applied at 22 adult dogs, 11 males and 11 females. Previously, the DAC and H of all animals were measured. For ultrasonographic examination, the ultra-sound system HDI 4000 PHILIPS equipped with a multi-frequency microconvex transducer, Color Doppler and Spectral Doppler devices was used. The animals were placed into right or left lateral decubitus position, in agreement with the kidney to be assessed. The longitudinal (LD) and dorsoventral diameters (DVD) of kidney were measured in longitudinal plane, and the transverse diameter (TD) was determined in transversal section. The renal volume (V) was automatically calculated by the ultrasound software. With Triplex Doppler, the RI and PI of right and left main renal arteries were obtained. All data were represented in mean ± SEM. Linear regression analyses were performed with renal LD, DVD, TD and V as dependent variable, and ACD and H as independent variable. RI and PI were compared between right and left renal arteries with Student's t-test. The LD, DVD, TD (cm) and V (ml) mean measurements for the left and right kidneys were: 5.24±0.27, 3.07±0.15, 3.07±0.9, 28.01±3.4 and 4.50±0.19, 2.88±0.14, 2.71±0.15, 21.27±2.6. All regression analyses were significant for the intercept and regression coefficient (P<0.01). There were statistical differences on RI and PI means between right and left renal arteries (P=0.001). The RI and PI means of left and right renal artery obtained were: 0.62±0.08 and 1.34±0.18; 0.70±0.06 and 1.62±0.13. The data obtained in the present paper can be used as parameters for evaluation of the renal size, volume and perfusion in adult dogs.