Navegando por Autor "BRITO, Maysa de Vasconcelos"

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Acesso aberto (Open Access)
Avaliação imunohistoquímica da densidade de vasos e expressão de moléculas de adesão celular da microvasculatura de lesões na doença de Jorge Lobo
(Universidade Federal do Pará, 2013-03-27) BRITO, Maysa de Vasconcelos; QUARESMA, Juarez Antônio Simões; http://lattes.cnpq.br/3350166863853054
Jorge Lobo's disease is a rare mycosis of chronic inflammation that causes injury to the skin without visceral dissemination. This disease is caused by the fungus Lacazia loboi. Its occurrence is prevalent in regions of hot and humid, with most cases reported in the Brazilian Amazon region. The histopathological findings showed lots of fungi at the site of injury, with a rich macrophages infiltrate with giant cells and limited presence of lymphocytes. The migration of leukocytes to the inflammatory site induced Lacazia loboi is supposedly co-ordinated by cytokines and chemokines that aided by blood and lymph vessels influence cell migration inducing the expression of adhesion molecules. In this paper we investigate possible microvascular changes associated with infection by Lacazia loboi at the site of injury that may interfere with the clinical evolution of patients. Therefore, we assessed the density of blood vessels and lymphatic vessels, as well as expression of molecules ICAM-1, VCAM-1 and E-selectin. Our results showed that in Jorge Lobo's disease, there is a reduced amount of blood and lymph vessels, when compared to control skin. There was a larger number of vessels expressing ICAM-1, being also higher number of vessels expressing the molecule VCAM-1, although in much less prominent ICAM-1. There were no differences in the expression of E-selectin. Together the results point to a change in the local microvasculature which may interfere with the development of an effective cellular immune response and justify the presence of the fungus confined to the injury site.
Acesso aberto (Open Access)
Environmental influences on antibody-enhanced dengue disease outcomes
(2012-12) DINIZ, Daniel Guerreiro; FÔRO, César Augusto Raiol; TURIEL, Maíra Catherine Pereira; SÓSTHENES, Márcia Consentino Kronka; DEMACHKI, Samia; GOMES, Giovanni Freitas; REGO, Carla M Damasceno; MAGALHÃES, Marina Cutrim; PINHO, Brunno Gomes; RAMOS, Juliana Pastana; CASSEB, Samir Mansour Moraes; BRITO, Maysa de Vasconcelos; SILVA, Eliana Vieira Pinto da; NUNES, Márcio Roberto Teixeira; DINIZ JUNIOR, José Antônio Picanço; CUNNINGHAM, Colm; PERRY, Victor Hugh; VASCONCELOS, Pedro Fernando da Costa; DINIZ, Cristovam Wanderley Picanço
Because an enriched environment (EE) enhances T-cell activity and T-lymphocytes contribute to immunopathogenesis during heterologous dengue virus (DENV) infections, we hypothesised that an EE increases dengue severity. To compare single serotype (SS) and antibody-enhanced disease (AED) infections regimens, serial intraperitoneal were performed with DENV3 (genotype III) infected brain homogenate or anti-DENV2 hyperimmune serum followed 24 h later by DENV3 (genotype III) infected brain homogenate. Compared AED for which significant differences were detected between the EE and impoverished environmental (IE) groups (Kaplan-Meyer log-rank test, p = 0.0025), no significant differences were detected between the SS experimental groups (Kaplan-Meyer log-rank test, p = 0.089). Survival curves from EE and IE animals infected with the AED regimen were extended after corticoid injection and this effect was greater in the EE than in the IE group (Kaplan-Meyer log-rank test, p = 0.0162). Under the AED regimen the EE group showed more intense clinical signs than the IE group. Dyspnoea, tremor, hunched posture, ruffled fur, immobility, pre-terminal paralysis, shock and death were associated with dominant T-lymphocytic hyperplasia and presence of viral antigens in the liver and lungs. We propose that the increased expansion of these memory T-cells and serotype cross-reactive antibodies facilitates the infection of these cells by DENV and that these events correlate with disease severity in an EE.