Navegando por Autor "BRITO JUNIOR, Lacy Cardoso de"

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Análise crítica dos achados hematológicos e sorológicos de pacientes com suspeita de Dengue
(2008-10) BARROS, Lilian Patrícia Souza; IGAWA, Sônia E. S.; JOCUNDO, Susana Y.; BRITO JUNIOR, Lacy Cardoso de
The increase in the number of more serious forms of dengue fever among cases in the city of Belém, Brazil has astounded local authorities. The objective of this study was to conduct a critical analysis of hematologic and serologic findings of patients with clinical suspicion of dengue seen at a clinical laboratory in Belém in Pará State. This retrospective study involved 210 patients who were referred to the Laboratory of Clinical Pathology Dr. Paulo C. Azevedo in Belém, in the period of February and March of 2007, with requests for a complete blood count and serological test for IgM to confirm the diagnosis of dengue. Of the cases studied, 51/210 (24.3%) presented with thrombocytopenia and 53/210 (25.2%) leukopenia. The serologic test for IgM was positive in 47.1% (99/210) of cases. A statistically significant association (p<0.05) was observed only among patients who presented with thrombocytopenia (33/99) and positive serology for dengue, suggesting that the hematological alterations of leukopenia and thrombocytopenia, frequently associated with this illness, may not be present at the beginning of the infection. It is therefore essential to carry out a serologic test for IgM to confirm a diagnosis of dengue.
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Diagnóstico de imunofenótipos de síndromes linfoproliferativas crônicas por citometria de fluxo na Fundação HEMOPA
(2011-12) BRITO JUNIOR, Lacy Cardoso de; FEIO, Danielle Cristinne Azevedo; BARBOSA, Suane Reis; BENTES, Alessandra Quinto; FRANCÊS, Larissa Tatiane Martins
INTRODUCTION: Lymphoproliferative syndromes comprise a heterogeneous group of malignant neoplasias with different clinical behaviors, pathological factors and epidemiological characteristics, whose diagnosis may be based on lymphoid cell morphology observed in peripheral blood. OBJECTIVE: To test the diagnostic feasibility of immunophenotyping by flow cytometry for lymphoproliferative syndromes through the definition of minimal antibody panel. MATERIAL AND METHODS: During the period of July 2008 to July 2010, 47 patients from HEMOPA blood center participated in this study for differential diagnosis of lymphoproliferative syndromes subtypes by flow cytometry. RESULTS: The mean age was 68 years old. There was no statistical difference between genders, and the most frequent subtype of lymphoproliferative syndromes was chronic lymphoid leukemia/small B-cell lymphocytic lymphoma. CONCLUSION: Based on the antibody panel recommended in this investigation, the immunophenotyping method by flow cytometry associated with morphological characterization of peripheral blood samples is a reliable, rapid, feasible, and non-invasive procedure for the diagnosis of chronic lymphoproliferative syndromes.
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Frequency of acute myeloid leukemia in children attended in Belém, Pará from August 2005 to May 2009
(2015-04) BRITO JUNIOR, Lacy Cardoso de; LEVY, Ian Eliezer; FRANCÊS, Larissa Tatiane Martins; WANDERLEY, Alayde Vieira; CARNEIRO, Rita de Cassia Matos; BENTES, Alessandra Quinto
Introduction: Acute myeloid leukemia (AML) has variable incidence in different regions of Brazil. Objective: To determine the frequency of AML subtypes in children aged 0-17 years attended at Belém, Pará, from August 2005 to May 2009. Patients and methods: A retrospective study was performed with 278 patients diagnosed with acute or chronic leukemia based on clinical and morphological criteria (French-American-British [FAB]/World Health Organization classification [WHO]) and immunophenotyping profile by flow cytometry, to determine the frequency of the subtypes in AML. Results: We found 70 (25.18%) cases of AML, 37 of these (52.9%) were children aged 0-17 years (median age of 7 years and 8 months). There was no statistical difference in relation to gender. We observed a higher frequency of AML subtype M2 (18/37 - 48.6%) and M0/M1 (10/37 - 27%), especially in the first decade of life (16/28 [57.1%] AML M2 and 9/28 [32.1%] AML M0/M1). Conclusion: In the pediatric population, the types of AML M2, M0/M1 and M3 were respectively the most frequent.
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Frequency of paroxysmal nocturnal hemoglobinuria in patients attended in Belém, Pará, Brazil
(2011-02) BRITO JUNIOR, Lacy Cardoso de; CARDOSO, Maria do Socorro de Oliveira; ROCHA, Euzamar Gaby; ANIJAR, Herika; CUNHA, Mariana; SARAIVA, João Carlos Pina
Paroxysmal nocturnal hemoglobinuria is a hematological disease with complex physiopathology. It is genetically characterized by a somatic mutation in the PIG-A gene (phosphatidylinositol glycan anchor biosynthesis, class A), in which the best known antigens are DAF (decay accelerating factor or CD55) and MIRL (membrane inhibitor of reactive lysis or CD59). OBJECTIVE: To determine the frequency of paroxysmal nocturnal hemoglobinuria in patients attended at the HEMOPA foundation from November 2008 to July 2009. METHOD: Thirty patients, with ages ranging from two to 79 years old and suspected of having paroxysmal nocturnal hemoglobinuria were examined. All patients were immunophenotyped by flow cytometry for the CD5, CD59, CD16 and CD45 antigens. RESULTS: Paroxysmal nocturnal hemoglobinuria was identified in nine of the thirty patients investigated. Another 3 cases had inconclusive results with CD59-negative labeling only for neutrophils. The highest frequency of paroxysmal nocturnal hemoglobinuria patients (7/9) and inconclusive cases (2/3) were between 19 years old and 48 years old, with a median of 28 years. CONCLUSION: These results show the importance of flow cytometry to identify cases in which patients are deficient in only one antigen (CD59).
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Importância da presença de granulações tóxicas para o diagnóstico hematológico de septicemia
(2007-12) SALGADO, Danielle Nazaré da Silva; CARVALHO, Raimundo Gladson Corrêa; OLIVEIRA, Maria de Fátima Pinto; SANTOS, Eduardo José Melo dos; BRITO JUNIOR, Lacy Cardoso de
This work aims at investigating the association of the presence of toxic granulation with positive blood cultures, age of patients, conditions of hospitalization and types of bacterial agents. Blind prospective and retrospective, analyses were carried out for the presence of toxic granulations-in blood samples of 300 patients of the both genders hospitalized in the City of Belém, Pará, Brazil. Request blood tests over a two year period were evaluated. The blood tests and cultures were performes using automated methods. All the data were statistically compared using the Qui-square test (clump method). The results show statistical associations between: (1) the presence of toxic granulations and positive blood cultures; (2) lower ages of patients (the newborn) and positive blood cultures; (3) hospitalization in the ICU and positive blood cultures and (4) toxic granulations-and the observation of leucocytosis and right-left shunts in patients hospitalized in the ICU with positive blood cultures. The commonest bacterial agents identified were klebsiella oxytoca (22%), Acinetobacter calcoaceticus (20%), Escherichia coli (18%), Enterobacter cloacae (14%), and Pseudomonas aeruginosa (8%).
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Nota técnica n. 03/2022/UFPA/PPGAC. O impacto da automação na fenotipagem eritrocitária estendida para a rotina de bancos de sangue
(Universidade Federal do Pará, 2022) CARNEIRO, Luciana Corrêa; BRITO JUNIOR, Lacy Cardoso de; AMARAL, Carlos Eduardo de Melo
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Nota técnica n. 04/2022/UFPA/PPGAC. Apresentação de uma tabela de consulta rápida para dispensação de concentrados de hemácias
(Universidade Federal do Pará, 2022) RAMOS, Neidiane Farias; BRITO JUNIOR, Lacy Cardoso de
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Nota técnica n. 05/2022/UFPA/PPGAC. Vantagens do teste de fragilidade osmótica com amostras analisadas após incubação por 24h a 37º c em banho-maria
(Universidade Federal do Pará, 2022-07) QUADROS, Alene de Oliveira; BRITO JUNIOR, Lacy Cardoso de
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Relato de caso de leucemia de células pilosas
(2011-12) BRITO JUNIOR, Lacy Cardoso de; BARBOSA, Suane Reis; FRANCÊS, Larissa Tatiane Martins
Hairy cell leukemia (HCL) is a rare type of B-cell non-Hodgkin's lymphoma. The clinical symptoms include splenomegaly, pancytopenia, and lymphocytosis. Studies on its carcinogenesis reveal association with exposure to agricultural chemical agents. The objective of this study was to report the case of a male patient, tractor operator, diagnosed with HCL, pancytopenia, cutaneous lesions, without splenomegaly and positive markers for B-cell lymphocytes (CD19, CD20, CD22, CD79b, CD23, Lambda, immunoglobulin M [IgM], CD25 and CD103). Although HCL is a rare disease, late diagnosis may ultimately lead to severe complications and patient's death.
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Tabus alimentares em medicina: uma hipótese para fisiopatologia referente aos alimentos remosos
(Universidade Federal do Pará, 2013-06) BRITO JUNIOR, Lacy Cardoso de; ESTACIO, Adriana Guimarães
Introduction: Human eating habits in the Amazon, especially in riparian communities, include a series of dietary restrictions (taboos) resulting, in part, from the cultural miscegenation (Native Brazilians, Africans, and Portuguese settlers) that occurred during the formation of this population.1 Among these food taboos, the most important refers to foods considered “remosos” (harmful), an adjective attributable to foods that have “reima”, i.e., that affect the blood and cause itching.2 In the popular Amazon vocabulary, “remosos” foods are heavy foods derived from pork; seafood such as crab, shrimp, scaleless fish; and cascudos, such as the tamuata; birds such as ducks; and some wild animals such as lowland pacas and capybaras. These foods should not be eaten by people at risk, for example, postoperative patients and people with infections, inflammations, or injuries, because of the risk of increasing tissue damage, creating pus, and exacerbating the inflammatory process.3,4 This recommendation, although not fully accepted by local physicians, is frequently made by some local doctors to postoperative patients. However, there are very few studies evidencing the pathophysiology of the effect of these foods on healing and inflammatory processes. Thus, the objective of this study was to promote a short review about the topic of foods considered “remoso” and to propose a theoretical hypothesis for this phenomenon based on fundamentals of immunology and of the Amazon ecosystem in order to scientifically help physicians to understand this phenomenon and treat patients from these regions.