Navegando por Autor "CAMPOS, Marliane Batista"

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Acesso aberto (Open Access)
Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs
(2010-10) LIMA, Luciana Vieira Rego; CARNEIRO, Liliane Almeida; CAMPOS, Marliane Batista; CHAGAS, Eujênia Janis; LAURENTI, Márcia Dalastra; CORBETT, Carlos Eduardo Pereira; LAINSON, Ralph; SILVEIRA, Fernando Tobias
Canine visceral leishmaniasis (CVL) is recognizable by characteristic signs of disease and is highly lethal. The infection, however, may be quite inapparent in some seropositive dogs, and this has raised the polemic question as to whether or not such animals can be a source of infection for Lutzomyia longipalpis, the vector of American visceral leishmaniasis (AVL). In this study we have examined 51 dogs with acute CVL from an AVL area in Pará State, northern Brazil, and compared the parasite density, amastigotes of Leishmania (L.) infantum chagasi, in the skin, lymph node and viscera of symptomatic with that of nine asymptomatic but seropositive dogs (IFAT-IgG). Post-mortem biopsy fragments of these tissues were processed by immunohistochemistry, using a polyclonal antibody against Leishmania sp. The X2 and Mann Whitney tests were used to evaluate the means of infected macrophage density (p < 0.05). There was no difference (p > 0.05) in the skin (10.7/mm2 x 15.5/mm2) and lymph node (6.3/mm2 x 8.3/mm2), between asymptomatic and symptomatic dogs, respectively. It was higher (p < 0.05), however, in the viscera of symptomatic (5.3/mm2) than it was in asymptomatic (1.4/mm2) dogs. These results strongly suggest that asymptomatic or symptomatic L. (L.) i. chagasi-infected dogs can serve as a source of infection, principally considering the highest (p < 0.05) parasite density from skin (10.7/mm2 x 15.5/mm2), the place where the vetor L. longipalpis takes its blood meal, compared with those from lymph node (6.3/mm2 x 8.3/mm2) and viscera (1.4/mm2x 5.3/mm2).
Acesso aberto (Open Access)
Estudo in vitro da infectividade de espécies de Leishmania do subgênero Viannia em macrófagos peritoneais de camundongo BALB/c
(Universidade Federal do Pará, 2003) CAMPOS, Marliane Batista; GOMES, Claudia Maria de Castro; http://lattes.cnpq.br/8660496919187291; SILVEIRA, Fernando Tobias; http://lattes.cnpq.br/8106158306299969
Parasites ofthe genus Leishmania show both intra- and inter-specific variations of infectivity. There is little available information, however, regarding the infective behavior of New World species particularly those of the Amazon Region of Brazil, where there occur at six species of the subgenus Viannia causing human cutaneous leishmaniasis (ACL). The aim of the present study was to investigate the infectivity of 5 of these species for the peritoneal macrophages of BALB/c mice, and their role in the host-cell's production of nitric oxide (NO). Thirty strains of Leishmania were divided into 6 groups of the fol1owing species: I-L. (V.) braziliensis (from cases of localized skin lesions-LCL); II- L. (V.) braziliensis (from cases of mucocutaneous lesions- LeM); III- L. (V.) guyanensis; IV- L. (V.) shawi; V- L. (V.) naiffi and VI- L. (V.) lainsoni. They were cultivated in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/gentamicin, until reaching the stationary phase of development. These promastigotes were used to infect the macrophage cell cultures, in the proportion of 4 flagellates/macrophage. The cultures were then incubated at 35°C with 5% CO2 and, after 24 hours following inoculation, the corvelips were stained by Giemsa's method to determine the infection index. The nitric oxide (nitrite) concentration was measured in the culture supernatant by Griess' s method to determine the infection index. The nitric oxide (nitrite) concentration was measured in the culture supernatant by Griess' s method. It was found that LCM strains of L. (V.) braziliensis showed the highest infection index (385), significance p<0.05,compared with L. (V.) braziliensis LCL strains (264). L. (V). naiffi and L. (V.) lansoni had the lowest infection indices of (215) and (272), respectively, but, were not significally different from index of L. (V.) guyanensis (300). Rrgarding the NO levels, the highest was that for L. (V.) naiffi (4,1µM), and the lowest for L. (V.) braziliensis-LCM strains (2, 15µM). The other readings were (3,14µM) for L. (V.) lainsoni, 2,96µM for L. (V.) shawi, 2,76µM for L. (V.) guyanensis and 3,1µM for L. (V.) braziliensis-LCL strains. It is conc1uded that L. (V.) natffi. In this way it may be noted the NO levels for infected macrophage were inversely proportional to the degree of infectivity of the species studied.
Acesso aberto (Open Access)
Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection
(2011-02) CARNEIRO, Liliane Almeida; SILVEIRA, Fernando Tobias; CAMPOS, Marliane Batista; BRÍGIDO, Maria do Carmo de Oliveira; CORBETT, Carlos Eduardo Pereira; LAURENTI, Márcia Dalastra
In Amazonian Brazil, the Cebus apella monkey (Primates: Cebidae) has been associated with the enzootic cycle of Leishmania (V.) shawi, a dermotropic parasite causing American cutaneous leishmaniasis (ACL). It has also been successfully used as animal model for studying cutaneous leishmaniasis. In this work, there has been investigated its susceptibility to experimental Leishmania (L.) infantum chagasi-infection, the etiologic agent of American visceral leishmaniasis (AVL). There were used ten C. apella specimens, eight adult and two young, four males and six females, all born and raised in captivity. Two experimental infection protocols were performed: i) six monkeys were inoculated, intra-dermal via (ID), into the base of the tail with 2 x 106 promastigotes forms from the stationary phase culture medium; ii) other four monkeys were inoculated with 3 x 107 amastigotes forms from the visceral infection of infected hamsters by two different via: a) two by intravenous via (IV) and, b) other two by intra-peritoneal via (IP). The parameters of infection evaluation included: a) clinical: physical exam of abdomen, weigh and body temperature; b) parasitological: needle aspiration of the bone-marrow for searching of amastigotes (Giemsa-stained smears) and promastigotes forms (culture medium); c) immunological: Indirect fluorescence antibody test (IFAT) and, Delayed-type hypersensitivity (DTH). In the six monkeys ID inoculated (promastigotes forms) all parameters of infection evaluation were negative during the 12 months period of follow-up. Among the four monkeys inoculated with amastigotes forms, two IV inoculated showed the parasite in the bone-marrow from the first toward to the sixth month p.i. and following that they cleared the infection, whereas the other two IP inoculated were totally negative. These four monkeys showed specific IgG-antibody response since the third month p.i. (IP: 1/80 and IV: 1/320 IgG) toward to the 12th month (IP: 1/160 and IV: 1/5120). The DTH-conversion occurred in only one IV inoculated monkey with a strong (30 mm) skin reaction. Considering these results, we do not encourage the use of C. apella monkey as animal model for studying the AVL.
Acesso aberto (Open Access)
Susceptibility of peritoneal macrophage from different species of neotropical primates to Ex vivo Leishmania (L.) infantum chagasi-infection
(2012-04) CARNEIRO, Liliane Almeida; LAURENTI, Márcia Dalastra; CAMPOS, Marliane Batista; GOMES, Claudia Maria de Castro; CORBETT, Carlos Eduardo Pereira; SILVEIRA, Fernando Tobias
This study examined the susceptibility of peritoneal macrophage (PM) from the Neotropical primates: Callithrix jacchus, Callithrix penicillata, Saimiri sciureus, Aotus azarae infulatus and Callimico goeldii to ex vivo Leishmania (L.) infantum chagasi-infection, the etiological agent of American visceral leishmaniasis (AVL), as a screening assay for evaluating the potential of these non-human primates as experimental models for studying AVL. The PM-susceptibility to infection was accessed by the PM-infection index (PMI) at 24, 72 h and by the mean of these rates (FPMI), as well as by the TNF-α, IL-12 (Capture ELISA) and Nitric oxide (NO) responses (Griess method). At 24h, the PMI of A. azarae infulatus (128) was higher than those of C. penicillata (83), C. goeldii (78), S. sciureus (77) and C. jacchus (55). At 72h, there was a significant PMI decrease in four monkeys: A. azarae infulatus (128/37), C. penicillata (83/38), S. sciureus (77/38) and C. jacchus (55/12), with exception of C. goeldii (78/54). The FPMI of A. azarae infulatus (82.5) and C. goeldii (66) were higher than C. jacchus (33.5), but not higher than those of C. penicillata (60.5) and S. sciureus (57.5). The TNF-a response was more regular in those four primates which decreased their PMI at 24/72 h: C. jacchus (145/122 pg/mL), C. penicillata (154/130 pg/mL), S. sciureus (164/104 pg/mL) and A. azarae infulatus (154/104 pg/mL), with exception of C. goeldii (38/83 pg/mL). The IL-12 response was mainly prominent in A. infulatus and C. goeldii which presented the highest FPMI and, the NO response was higher in C. goeldii, mainly at 72 h. These findings strongly suggest that these New World primates have developed a resistant innate immune response mechanism capable of controlling the macrophage intracellular growth of L. (L.) i. chagasi-infection, which do not encourage their use as animal model for studying AVL.