Navegando por Autor "CARVALHO, Josiwander Miranda"

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Acesso aberto (Open Access)
Annularins I and J: New Metabolites Isolated from Endophytic Fungus Exserohilum rostratum
(Universidade Federal do Pará, 2016-08) PINHEIRO, Eduardo Antonio Abreu; BORGES, Fábio Cardoso; PINA, Jeferson Rodrigo Souza; FERREIRA, Leila do Remédio Santos; CORDEIRO, Jorgeffson da Silva; CARVALHO, Josiwander Miranda; FEITOSA, André de Oliveira; CAMPOS, Francinete Ramos; BARISON, Andersson; SOUZA, Afonso Duarte Leão de; MARINHO, Patrícia Santana Barbosa; MARINHO, Andrey Moacir do Rosário
The paper describes the isolation of ergosterol peroxide, monocerin, annularin I and annularin J compounds of Exserohilum rostratum biomass extracts. Compounds were isolated by silica gel column chromatography in polarity gradient and the structures were determined by nuclear magnetic resonance (NMR) and mass spectrometry (MS) methods. Monocerin, annularin I and annularin J compounds were tested against Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis bacteria and showed moderate activity. Annularin I and annularin J compounds are new natural products.
Acesso aberto (Open Access)
Chemical constituents of Aspergillus sp EJC08 isolated as endophyte from Bauhinia guianensis and their antimicrobial activity
(2013) PINHEIRO, Eduardo Antonio Abreu; CARVALHO, Josiwander Miranda; SANTOS, Diellem Cristina Paiva dos; FEITOSA, André de Oliveira; MARINHO, Patrícia Santana Barbosa; GUILHON, Giselle Maria Skelding Pinheiro; SANTOS, Lourivaldo da Silva; SOUZA, Afonso Duarte Leão de; MARINHO, Andrey Moacir do Rosário
The present work reports the isolation of five compounds from Aspergillus sp EJC08 isolated as endophytic from Bauhinia guianensis, a tipical plant of the Amazon. The compounds ergosterol (1), ergosterol peroxide (2), mevalolactone (3), monomethylsulochrin (4) and trypacidin A (5) were isolated by chromatographic procedures and identified by spectral methods of 1D and 2D NMR and MS. Compounds 3, 4 and 5 were tested against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus and showed good activity.
Acesso aberto (Open Access)
Phytosterols isolated from endophytic fungus Colletotrichum gloeosporioides (Melanconiaceae)
(Instituto Nacional de Pesquisas da Amazônia, 2016-03) CARVALHO, Josiwander Miranda; PAIXÃO, Luanna Karyna Olimpio da; DOLABELA, Maria Fâni; MARINHO, Patrícia Santana Barbosa; MARINHO, Andrey Moacir do Rosário
Endophytic fungi are fungi that colonize internal tissues of plants. There are few studies of compounds isolated from endophytic fungi of Amazon plants. Thus, the aim this study was the isolation and structural identification of sitosterol (1), stigmasterol (2), sitostenone (3), squalene (4), ergosterol (5) and ergosterol peroxide (6) from fungus Colletotrichum gloeosporioides isolated as endophytic from Virola michelli, a typical Amazon plant, used in folk medicine against skin infection. Compounds were isolated by chromatography column on silica and identified by 1 H and 13C NMR and MS. The presence of phytosterols in fungi is rare and this is the first report of the isolation of the phytosterols sitosterol, stigmasterol and sitostenone from the genus Colletotrichum.