Navegando por Autor "CARVALHO, Milene Alves de Figueiredo"
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Item Acesso aberto (Open Access) Indução, análises morfológicas e ultraestruturais de calos de maracujazeiro nativo(Universidade Federal do Pará, 2015-08) CARVALHO, Milene Alves de Figueiredo; PAIVA, Renato; HERRERA, Raírys Cravo; ALVES, Eduardo; CASTRO, Evaristo Mauro de; PAIVA, Patrícia Duarte de Oliveira; VARGAS, Daiane PeixotoAnalysis of calluses with embryogenic characteristics is important for subsequent in vitro regeneration of species with desirable agronomic traits such as the Passiflora gibertii native passion fruit. In this context, the present study aimed to analyze the induction of calluses from leaf explants of Passiflora gibertii N. E. Brown, as well as their morphologic and ultra-structural characterization. Cotyledonary leaves were inoculated in culture medium containing 2,4-D and picloram in association with kinetin. After 30 days of cultivation in the dark, the obtained calluses were prepared for the visualization in electron microscope (transmission and scanning) and light microscope. The results allowed to infer that the addition of picloram and kinetin to the culture medium promotes higher callus formation in cotyledonary leaf explants of P. gibertii than 2,4-D and kinetin. The growth regulator 2,4-D provides the formation of calluses with small isodiametric cells and small intercellular spaces, an organized cellular system and the predominance of round shaped mitochondria. Predominance of large and elongated cells with intercellular spaces, a non-organized cellular system and predominance of elongated mitochondria was observed when picloram was used.Item Acesso aberto (Open Access) Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit(2014-12) CARVALHO, Milene Alves de Figueiredo; PAIVA, Renato; STEIN, Vanessa Cristina; HERRERA, Raírys Cravo; PORTO, Jorge Marcelo Padovani; VARGAS, Daiane Peixoto; ALVES, EduardoThis work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.