Navegando por Autor "CIPRANDI, Alessandra"
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Item Acesso aberto (Open Access) Análise proteômica da resposta ao arsênio e do exoproteoma de Chromobacterium violaceum(Universidade Federal do Pará, 2011-07-06) CIPRANDI, Alessandra; SILVA, Artur Luiz da Costa da; http://lattes.cnpq.br/7642043789034070Chromobacterium violaceum is a Gram-negative beta-proteobacterium found in tropical ecosystems and it is an opportunistic pathogen for animals and humans. C. violaceum infection is associated with a high mortality rate, but little is known about the molecular basis of pathogenicity mechanisms. As an environmental microorganism, C. violaceum is exposed to diverse external conditions, which require great adaptability and effective protection systems. C. violaceum possesses an arsenic resistance operon arsRBC. Arsenic is a toxic metalloid associated with skin lesions, neurological diseases and cancer. The aim of this study was to investigate changes in protein pattern in presence of arsenite and characterize secreted proteins of C. violaceum ATCC 12472. The proteins from C. violaceum were analyzed by twodimensional electrophoresis and mass spectrometry. Proteomic analysis revealed that arsenite induces an increase of proteins involved in oxidative stress response, DNA repair and energetic metabolism. Among the secreted proteins were identified virulence factors (metallopeptidases, collagenase and toxins), transporters, and proteins involved in stress response and potentially useful. The results show novel insights into the adaptive response of C. violaceum.