Navegando por Autor "DANTAS, Vanderson Vasconcelos"

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Acesso aberto (Open Access)
Caracterização dos sistemas de produção na bovinocultura leiteira nas mesorregiões sudeste e nordeste paraense
(Universidade Federal do Pará, 2014-02-25) DANTAS, Vanderson Vasconcelos; GODOY, Bruno Spacek; http://lattes.cnpq.br/4036516695601666; OAIGEN, Ricardo Pedroso; http://lattes.cnpq.br/8056365542183068
The dairy industry is of great importance in the regional economy in terms of employment and income generation , especially for family farming . Know the reality of these systems becomes essential so that they can generate specific grants for further development of the activity. In this context, studies on the different production systems emerge as an important tool. The objective of this study systems of milk production was characterized in the regions of Southeast and Northeast Pará through the use of multivariate analysis techniques . The survey was conducted through questionnaires with 112 properties located in the municipalities of Ulianópolis - PA and PA - Irituia . Data were tabulated in Microsoft Excel and processed using SPSS 18.0 software . Through frequency analysis revealed that the total area of varied properties 7-1600 ha . The owners were ranked according to the number of cows in the existing properties and the lowest represented by those with less than 10 cows, which corresponded to 5.35 % and higher over 50 ( 23.21 %). Regarding herd productivity observed an overall average of 4.34 ( L cow -1 day -1) . Feeding the flock is based on cultivated pastures , being the most used braquiarão . Only 19.60% of the properties held supplementation with concentrate and roughage with 13.39% . The reproductive management is performed in 86.6 % through natural mating without any control , which results in little specialized animals for dairy production . The management of milking presents itself as the poor hygienic and sanitary conditions , resulting in a poor quality product . Management practices show little expression among producers . With respect to the typology established through factor analysis revealed four factors that explained 66.99 % of the original variables , and the cluster analysis identified 4 groups of producers with similar profiles . The group III corresponded to the group of producers that had the highest adoption of technology such as supplemental feeding , health management , milking and activity management technology, thus having better performance with an average productivity of 5.02 ( L cow -1 day -1) . Groups I and II are characterized by family production systems with low specialization and low productivity and group IV consists of family farmers who presented with an incipient share of agriculture production , having the lowest production and economic returns.
Acesso aberto (Open Access)
Reação em cadeia da polimerase multiplex (mPCR) para detecção de fraude e identificação de salmonella spp. em amostras de carne bovina e bubalina
(Universidade Federal do Pará, 2019-09-20) DANTAS, Vanderson Vasconcelos; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769
The authenticity of food has become a major issue today, for economic reasons, lifestyle, health and religious beliefs, as well as a requirement of food regulators. Proper labeling of a meat product according to its identity and quality standard is essential to ensure the food safety of consumers. However, the identification of meat and meat constituents is still a challenge for the authorities, as it is not always possible to authenticate the species of origin using commonly used methods, especially in cases where adulteration by the addition of material occurs. from a species other than those indicated on the label. The aim of this study was to optimize and develop different Multiplex Polymerase Chain Reaction (mPCR) protocols for simultaneous identification of bovine and buffalo species and for identification of Salmonella spp. in commercially available meat cuts. Two methods of DNA extraction were applied (commercial kit and from the use of organic solvents) and the quality / quantity of the obtained material was determined by agarose gel electrophoresis and spectrophotometry. Primers that amplify sequences of 429 base pairs for Salmonella spp. DNA, 346 base pairs for bovine DNA and 220 base pairs for buffalo DNA were used to perform the proposed mPCR. dilution of the extracted template DNA. For the verification of specificity, DNAs from different animal species and microorganisms were used. After standardization of the technique, bovine and buffalo meat samples were artificially contaminated with standard Salmonella tiphymurium strain (ATCC 14028), so that the detection limit of the technique was determined and, finally, commercial samples of meat cuts commercialized in northern Brazil. Brazil were analyzed by this technique to verify the hypothesis of fraud and the presence of Salmonella spp. in products marketed in the target region of the study. The results showed that the proposed mPCR presented adequate sensitivity and specificity, being able to detect Salmonella spp. from 106 cfu/mL after 12h enrichment. In addition, it was observed that approximately 20% of the samples marketed as bovine origin were of bubaline origin, and of this total, 31% had Salmonella spp. It was concluded that The developed mPCRs are efficient to detect fraud in meat cuts and Salmonella spp. It may be an alternative to be used in the routine inspection of these products.