Navegando por Autor "DINIZ, Daniel Guerreiro"

Agora exibindo 1 - 10 de 10

Acesso aberto (Open Access)
Effects of chromium supplementation on the infrapopulations of Anacanthorus penilabiatus (Monogenoidea) and Piscinoodinium pillulare (Dinoflagellida) parasites of Piaractus mesopotamicus (Characidae)
(2010-08) FUJIMOTO, Rodrigo Yudi; CASTRO, Marcello Pardi de; MARTINS, Maurício Laterça; MORAES, Flávio Ruas de; VARELLA, Jamille Elzira de Almeida; DINIZ, Daniel Guerreiro
This study evaluated the parasitism by Anacanthorus penilabiatus (Monogenoidea) and Piscinoodinium pillulare (Dinoflagellida) in the gills of "pacu", Piaractus mesopotamicus supplemented with chromium in the diet. Randomised delineation by using factorial scheme (4x2x2) for Monogenoidea and 4x2x3 for Dinoflagellate with four levels of chromium (0, 6, 12, 18mg/kg diet), two stocking densities (4kg/m3 and 20kg/m3), two classes of length (higher and lower than 17 cm) for a period of 7, 60 and 90 days, and four replicates was used. The fishes in low density which received 12 and 18mg/kg showed decreased monogeneoidea values, seven days after the experiment begin. Six, 12 and 18 mg/kg caused reduction in the dinoflagellate number in the fishes maintained at 20kg/m3. It was possible that chromium supplementation (12 e 18mg/kg) favoured the fishes health by reducing Monogenoidea and Dinoflagellida number in lower and higher densities, respectively.
Acesso aberto (Open Access)
Environmental influences on antibody-enhanced dengue disease outcomes
(2012-12) DINIZ, Daniel Guerreiro; FÔRO, César Augusto Raiol; TURIEL, Maíra Catherine Pereira; SÓSTHENES, Márcia Consentino Kronka; DEMACHKI, Samia; GOMES, Giovanni Freitas; REGO, Carla M Damasceno; MAGALHÃES, Marina Cutrim; PINHO, Brunno Gomes; RAMOS, Juliana Pastana; CASSEB, Samir Mansour Moraes; BRITO, Maysa de Vasconcelos; SILVA, Eliana Vieira Pinto da; NUNES, Márcio Roberto Teixeira; DINIZ JUNIOR, José Antônio Picanço; CUNNINGHAM, Colm; PERRY, Victor Hugh; VASCONCELOS, Pedro Fernando da Costa; DINIZ, Cristovam Wanderley Picanço
Because an enriched environment (EE) enhances T-cell activity and T-lymphocytes contribute to immunopathogenesis during heterologous dengue virus (DENV) infections, we hypothesised that an EE increases dengue severity. To compare single serotype (SS) and antibody-enhanced disease (AED) infections regimens, serial intraperitoneal were performed with DENV3 (genotype III) infected brain homogenate or anti-DENV2 hyperimmune serum followed 24 h later by DENV3 (genotype III) infected brain homogenate. Compared AED for which significant differences were detected between the EE and impoverished environmental (IE) groups (Kaplan-Meyer log-rank test, p = 0.0025), no significant differences were detected between the SS experimental groups (Kaplan-Meyer log-rank test, p = 0.089). Survival curves from EE and IE animals infected with the AED regimen were extended after corticoid injection and this effect was greater in the EE than in the IE group (Kaplan-Meyer log-rank test, p = 0.0162). Under the AED regimen the EE group showed more intense clinical signs than the IE group. Dyspnoea, tremor, hunched posture, ruffled fur, immobility, pre-terminal paralysis, shock and death were associated with dominant T-lymphocytic hyperplasia and presence of viral antigens in the liver and lungs. We propose that the increased expansion of these memory T-cells and serotype cross-reactive antibodies facilitates the infection of these cells by DENV and that these events correlate with disease severity in an EE.
Acesso aberto (Open Access)
First occurrence of Quadrigyrus nickoli (Acanthocephala) in the ornamental fish Hyphessobrycon eques
(2013-03) FUJIMOTO, Rodrigo Yudi; SARMENTO, Aline de Melo Braun; DINIZ, Daniel Guerreiro; EIRAS, Jorge Costa; MARTINS, Maurício Laterça
The objective of this work was to report the first seasonal occurrence of the acanthocephalan Quadrigyrus nickoli Schmidt & Hugghins, 1973 (Quadrigyridae), in the “Mato Grosso” Hyphessobrycon eques (Characidae) (Steindachner, 1882), collected from the Chumucuí River, state of Pará, Brazil. The fish were collected between July 2006 (rainy season) and June 2007 (dry season) and were examined for parasites using pattern techniques. A total of 75 parasites were found in the stomach and intestine. Among 83 fish examined (50 in the dry season and 33 in the rainy season), 22 were parasitized by cystacanths of Q. nickoli. The importance of H. eques as a paratenic host for Q. nickoli is discussed. This is the first study on the biology of and infection by Q. nickoli occurring in the eastern Amazon region.
Acesso aberto (Open Access)
First occurrence of Quadrigyrus nickoli (Acanthocephala) in the ornamental fishHyphessobrycon eques
(Colégio Brasileiro de Parasitologia Veterinária, 2013-03) FUJIMOTO, Rodrigo Yudi; ALMEIDA, Edilene Santos de; DINIZ, Daniel Guerreiro; MARTINS, Maurício Laterça; EIRAS, Jorge Costa
The objective of this work was to report the first seasonal occurrence of the acanthocephalan Quadrigyrus nickoliSchmidt & Hugghins, 1973(Quadrigyridae), in the “Mato Grosso”Hyphessobrycon eques (Characidae) (Steindachner, 1882), collected from the Chumucuí River, state of Pará, Brazil. The fish were collected between July 2006 (rainy season) and June 2007 (dry season) and were examined for parasites using pattern techniques. A total of 75 parasites were found in the stomach and intestine. Among 83 fish examined (50 in the dry season and 33 in the rainy season), 22 were parasitized by cystacanths of Q. nickoli. The importance of H. eques as a paratenic host for Q. nickoli is discussed. This is the first study on the biology of and infection by Q. nickoli occurring in the eastern Amazon region.
Acesso aberto (Open Access)
Influências da idade e do ambiente sobre o curso temporal da infecção pelo vírus da Dengue acentuada por anticorpo heterólogo em modelo murino: ensaios comportamentais e histopatológicos
(Universidade Federal do Pará, 2014-01-03) DINIZ, Daniel Guerreiro; DINIZ, Cristovam Wanderley Picanço; http://lattes.cnpq.br/2014918752636286
Because the enriched environment (EE) increases the activity of T cells, contribute to the immunopathogenesis of dengue virus infections (VDEN) we hypothesized that animals maintained in an enriched environment (AE) compared with animals from impoverished environment of standard laboratory cages (IE), would develop more severe forms of the disease. Because older animals have less functional decline in adaptive immunity T cells, we tested the hypothesis that AE old mice would show higher number of deaths and more intense clinical signs than age-matched IE animals, and this would be associated with greater expansion of T lymphocytes. To test these hypotheses we established scheme of multiple inoculations in adult animals of 9 and 18 months of age. Two regimens of inoculation were tested: multiple injections of single serotype (VDEN3 genotype III) infected brain homogenate (SS) or alternatively multiple injections with that infected brain homogenate followed 24h later by inoculation of heterologous antibody (SSHA). In both cases multiple i.p. inoculations were done. It was found significant differences in the temporal progression of the disease in the animals submitted to one or another scheme of inoculation: SSHA group (Kaplan -Meier log-rank test, p = 0.0025); IUS (Kaplan -Meier log-rank test, p = 0.089). The survival curves of AE and AP under SSHA regime were extended after a single injection of glucocorticoids, reducing the symptoms and the number of deaths, and these effects were greater in the EE group than in the IE (Kaplan-Meier log-rank test, p = 0.0162). In SSHA scheme, EE group showed clinical signs more intense than the AP and those included dyspnea, tremor, hunched posture, immobility, pre-terminal paralysis, shock and eventual death. Compared to the IE group, the AE group regardless of age showed higher mortality and more severe clinical signs. These more severe clinical signs in EE animals under SSHA regime were associated with increased hyperplasia of T lymphocytes in the spleen and increased infiltration of these cells in the liver, lungs and kidneys. Although lymphocytic hyperplasia and infiltration have been more intense in older than in younger animals, immunostaining for viral antigens in target organs was higher in young than in the aged mice. The presence of the virus in various infected organs were confirmed by real time PCR. Taken together the results suggest that the enriched environment life style exacerbates the subsequent inflammatory response to infection, and that is associated with more severe clinical symptoms, higher mortality and increased T cell expansion. Behavioral and histopathological data validate a new immunocompetent murine model for studies on dengue disease allowing in vivo tests of a number of hypothesis raised by epidemiological and in vitro studies.
Acesso aberto (Open Access)
Kudoa spp. (Myxozoa, Multivalvulida) parasitizing fish caught in Aracaju, Sergipe, Brazil
(Universidade Federal do Pará, 2016-12) EIRAS, Jorge Costa; FUJIMOTO, Rodrigo Yudi; MADI, Rubens Riscala; JERALDO, Veronica de Lourdes Sierpe; MELO, Cláudia Moura de; SOUZA, Jônatas dos Santos de; DINIZ, José Antonio Picanço; DINIZ, Daniel Guerreiro
This study reports on Kudoa spp. (Myxozoa, Multivalvulida) from the fish species Lutjanus analis, Bagre marinus, Aspistor luniscutis and Lutjanus jocu, which were caught in Aracaju, state of Sergipe, Brazil. The parasites formed oval plasmodia around the esophagus of L. analis, and elongated plasmodia inside the skeletal muscle of B. marinus, A. luniscutis and L. jocu. Host myoliquefaction was not observed in all the cases studied. The current study provides a morphological and morphometric description of each parasite as well as a comparison with all the species described worldwide. Lack of molecular data impaired specific identification of the parasites. The importance of these parasites is discussed and the need for further studies on infections in Brazilian fish is emphasized because of the high economic impact of some Kudoa species which cause liquefaction in hosts’ muscles and render these fish unsuitable for consumption.
Acesso aberto (Open Access)
Microglia and neurons in the hippocampus of migratory sandpipers
(Universidade Federal do Pará, 2015-11) DINIZ, Cristovam Guerreiro; MAGALHÃES, Nara Gyzely de Morais; SOUSA, Aline Andrade de; SANTOS FILHO, Carlos; DINIZ, Daniel Guerreiro; LIMA, Camila Mendes de; OLIVEIRA, Marcus Augusto de; PAULO, Dario Carvalho; PEREIRA, Patrick Douglas Corrêa; SHERRY, David Francis
The semipalmated sandpiper Calidris pusilla and the spotted sandpiper Actitis macularia are long- and short-distance migrants, respectively. C. pusilla breeds in the sub-arctic and mid-arctic tundra of Canada and Alaska and winters on the north and east coasts of South America. A. macularia breeds in a broad distribution across most of North America from the treeline to the southern United States. It winters in the southern United States, and Central and South America. The autumn migration route of C. pusilla includes a non-stop flight over the Atlantic Ocean, whereas autumn route of A. macularia is largely over land. Because of this difference in their migratory paths and the visuo-spatial recognition tasks involved, we hypothesized that hippocampal volume and neuronal and glial numbers would differ between these two species. A. macularia did not differ from C. pusilla in the total number of hippocampal neurons, but the species had a larger hippocampal formation and more hippocampal microglia. It remains to be investigated whether these differences indicate interspecies differences or neural specializations associated with different strategies of orientation and navigation.
Acesso aberto (Open Access)
Nematode parasites of Pescada Gó, Macrodon ancylodon Bloch and Schneider, 1801 (Osteichthyes, Sciaenidae), from Vila dos Pescadores, Bragança-PA, Brazil
(2012-12) FUJIMOTO, Rodrigo Yudi; SARMENTO, Aline de Melo Braun; DINIZ, Daniel Guerreiro; EIRAS, Jorge Costa
The objective of this work was to evaluate the host/parasite relationship of nematodes in Macrodon ancylodon. Ninety seven fishes (50 in wet season and 47 in dry season) were weighed (167.1 ± 109.9g) and measured (28.3 ± 5.2cm). Only twelve specimens were not infected by any parasites and the prevalence of infection was 87.6%. The highest prevalence values were observed in August and September (dry season, 100%). The stomach was the most infected organ during the whole months (prevalence of 64.2%, and mean intensity of 4.6±7.8 parasites/fish), and the medium intestine showed the lowest infection (prevalence 27.3% and mean intensity 2.5±2.1 parasites/fish). The nematodes were identified as Raphidascaris sp., Goezia sp. and Cucullanus sp. Only the male and juvenile fishes could be presented different values of infection according to rainfall, being more infected in August to October. The female hosts presented higher values of abundance and mean intensity of infection (p<0.01) throughout the year.
Acesso aberto (Open Access)
A note on the occurrence of praniza larvae of Gnathiidae (Crustacea, Isopoda) on fishes from Northeast of Pará, Brazil
(2008-12) DINIZ, Daniel Guerreiro; VARELLA, Jamille Elzira de Almeida; HOSHINO, Maria Danielle Figueiredo Guimarães; SANTOS, Arthur Felipe Lima dos; FUJIMOTO, Rodrigo Yudi; MONFORT, Karla Cibele Fragoso; PIRES, Marcus Alexandre Borges; MARTINS, Maurício Laterça; EIRAS, Jorge Costa
The infection of the estuarine teleost fishes Mugil gaimardianus Desmarest, 1831 (Mugilidae), Arius phrygiatus Valenciennes, 1839 (Ariidae), Conodon nobilis Linnaeus, 1759 (Haemulidae), Cetengraulis edentulus Cuvier, 1829 (Engraulidae), and Anableps anableps Linnaeus, 1758 (Anablepidae) by praniza larvae of Gnathiidae (Crustacea, Isopoda) was studied in specimens fished off the Atlantic Ocean in Northeast of Pará State, near Bragança, Brazil. The highest infection prevalence value was found in Anableps anableps (42.3%) and the lowest in Conodon nobilis (9.1%). The mean intensity varied from 1 parasitein Conodon nobilis to 19.5 in Arius phrygiatus. A description of the larvae is provided. The morphology of the mouthparts is related to the blood sucking activity, and is compared with the characteristics of other gnathiidae species.
Acesso aberto (Open Access)
Parasites of four ornamental fish from the Chumucuí River (Bragança, Pará, Brazil)
(2013-03) FUJIMOTO, Rodrigo Yudi; BARROS, Zaira Monik Nunes de; MARINHO FILHO, Adjalbas Nunes; DINIZ, Daniel Guerreiro; EIRAS, Jorge Costa
The objective of the present study was to evaluate the parasite fauna of four species of ornamental fish collected in the Chumucuí River, municipality of Bragança, Pará, Brazil. From June 2006 to December 2007. Fishes (n=307) belonging to four species were collected, including 23 specimens of Moenkhausia sanctaefilomenae (redeye tetra), 37 Carnegiella strigata (marbled hatchetfish), 7 Chilodus punctatus (spotted headstander), and 240 Astyanax bimaculatus (twospot astyanax). The parasites found belonged to three taxa: monogeneans in the gills, nematodes (larvae of Capillaria sp. and Contracaecum sp.) in the digestive tract and liver and acanthocephalans (Quadrigyrus torquatus, Q. brasiliensis and Q. nickoli) in the stomach and intestine. Astyanax bimaculatus presented higher prevalence of acanthocephalans in the wet season, and lower prevalence of nematodes in the dry season. The possible importance of these parasites in the exportation of ornamental fish is discussed.