Navegando por Autor "FECURY, Amanda Alves"

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Analysis of polymorphisms in the interleukin 18 gene promotor (-137 g/c and -607 c/a) in patients infected with hepatitis c virus from the brazilian amazon
(Universidade Federal do Pará, 2015-09) SANTOS, Kemper Nunes dos; ALMEIDA, Marcella Kelly Costa de; FECURY, Amanda Alves; COSTA, Carlos Araújo da; MARTINS, Luisa Caricio
Background - The hepatitis C virus has been recognized as the leading cause of chronic liver disease in the world. Host genetic factors have been implicated in the persistence of hepatitis C virus infection. Single nucleotide polymorphisms at positions -607 C/A (rs1946518) and -137 G/C (rs187238) in the IL-18 gene promoter have been suggested to be associated with delayed hepatitis C virus clearance and persistence of the disease. Objective - Identify these polymorphisms in a population infected with hepatitis C virus from the Brazilian Amazon region. Methods - In a cross-sectional analytical study conducted in Belém, Pará, Brazil, 304 patients infected with hepatitis C virus were divided into two groups: group A, patients with persistent infection; group B, patients with spontaneous clearance. The control group consisted of 376 volunteers not infected with hepatitis C virus. Samples were analyzed by RT-PCR for the detection of viral RNA and by RFLP-PCR to evaluate the presence of the -137 G/C and -607 C/A IL-18 gene promoter polymorphisms. Results - Comparison of polymorphism allele frequencies between the patient and control groups showed a higher frequency of allele C at position -607 among patients (P=0.02). When the association between the polymorphisms and viral infection was analyzed, patients carrying genotype C/A at position -607 were found to be at higher risk of persistent hepatitis C virus infection (P=0.03). Conclusion - the present results suggest a possible role of the -607 IL-18 gene promoter polymorphism in the pathogenesis of hepatitis C virus infection.
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Aspectos epidemiológicos da infecção pelo vírus da hepatite C em populações ribeirinhas do estado do Pará, na Amazônia brasileira
(Universidade Federal do Pará, 2015-09-24) FECURY, Amanda Alves; MARTINS, Luisa Caricio; http://lattes.cnpq.br/1799493244439769
The hepatitis C virus (HCV) has large number of infected and may cause hepatocellular carcinoma. Its genetic material RNA presents six different genotypes (1, 2, 3, 4, 5 and 6) and subtypes, related by disease prognosis and response to treatment. The main route of transmission of HCV is the parenteral route, but the vertical and intrafamily transmission and is also reported. Drug use and needlestick share were characterized as risk factors for acquisition of infection and pathways are described and confirmed by literature. Less is known about viral transmission in remote or hard to reach communities such as riverside communities. This study aimed to determine the prevalence of viral infection, evaluate the serological profile and circulating genotype HCV, and trace the epidemiological characteristics, describing the main risk factors for acquisition of HCV infection that different riverside communities living in the State of Pará, in the Eastern Amazon, are exposed. The respondents answered a questionnaire to obtain epidemiologic information and blood samples were collected to carry out the serological, molecular, and genotyping tests. 1.277 samples were collected from Surrounding of the Tucuruí Hydroeletric, Pacuí, Hole of Maracujá and Nazário communities. Predominant age group 18-37 years old, female, married, low education, fishermen or açaí extractor, with up to 1 minimum wage. Respecting the particular characteristics of each local, consumption of alcohol and tobacco ranged between communities, as well as sharing the spiked material and cutting, the manicure kits, surgeries, hospitalization, presence of sexually transmitted diseases and if has any familiar with hepatitis. The Pacuí presented prevalence of HCV infection of 8,84 % having moderate prevalence of infection and Tucuruí was 2,25 %, close to the national average. The genotype 1 was found in 100% of Pacuí samples and 70% of Tucuruí samples, which possessed the remaining 30% of the samples belonging to genotype 3, following the pattern expected genotype distribution. In Maracujá Hole and Nazário Hole, there were no positive samples. The communities seem to have individuals who should be considered for the taking of public health measures, with increased risk of infection through drug use and the sharing of needlestick objects. The viral circulation have intermediate to moderate prevalence, with standard genotypic distribution similar to the Brazilian. The distribution of the infection and its features can vary within the country and even within their own regions, depending on the presence and viral circulation, in addition to the risk factors to which comunities are exposed.
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Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil
(2005-12) MARTINS, Luisa Caricio; CORVELO, Tereza Cristina de Oliveira; DEMACHKI, Sâmia; ARAÚJO, Marialva Tereza Ferreira de; ASSUMPÇÃO, Mônica Baraúna de; VILAR, Simone Cristina Araujo Jucá; FREITAS, Felipe Bonfim; BARBOSA, Hivana Patricia Melo; FECURY, Amanda Alves; AMARAL, Renata Kelly Costa do; SANTOS, Sidney Emanuel Batista dos
We have examined the prevalence of gene cagA and vacA alleles in 129 patients, 69 with gastritis and 60 with peptic ulcer diseases from North Brazil and their relation with histopathological data. vacA and cagA genotype were determined by polymerase chain reaction. Hematoxylin-eosin staining was used for histological diagnosis. 96.6% of the patients were colonized by Helicobacter pylori strains harboring single vacA genotype (nont-mixed infection). Among them, 11.8% had subtype s1a, 67.8% had subtype s1b, and 17% subtype s2. In regard to the middle region analysis, m1 alleles were found in 75.4% and m2 in 21.2% of patients. The cagA gene was detected in 78% patients infected with H. pylori and was associated with the s1-m1 vacA genotype. The H. pylori strains, vacA s1b m1/cagA-positive, were associated with increased risk of peptic ulcer disease and higher amounts of lymphocytic and neutrophilic infiltrates and the presence of intestinal metaplasia. These findings show that cagA and vacA genotyping may have clinical relevance in Brazil.
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Helicobacter pylori infection and immune profile of patients with different gastroduodenal diseases
(Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades, 2018-06) VINAGRE, Ruth Maria Dias Ferreira; VINAGRE, Igor Dias Ferreira; SILVA, Adenielson Vilar e; FECURY, Amanda Alves; MARTINS, Luisa Caricio
The association between infection with Helicobacter pylori and different gastroduodenal diseases is related to bacterial, host and environmental factors. Studies have demonstrated an association between the genetic diversity of H. pylori, especially in the vacA and cagA genes, and the development of digestive diseases such as peptic ulcer and gastric cancer. In addition, the nature of the host inflammatory response may explain these different manifestations of infection caused by this microorganism. In this respect, host factors that regulate the immune and inflammatory responses involving the functional interaction of H. pylori infection with different components of the immune system, particularly T cells, in gastroduodenal diseases still need further investigation. Objective: To characterize the immune response, including immunity induced by infection with H. pylori, especially virulent strains (vacA alleles and cagA gene), by analyzing the cytokine profile and T-cell population present in gastroduodenal diseases in a Brazilian population. Methods: In a prospective study, gastric biopsies were collected from 554 patients with different gastroduodenal diseases for histological analysis and for the determination of bacterial genotype and cytokine production (IL-4, IL-10, IFN-γ and IL-12) by ELISA. Results: The predominant genotype of the H. pylori strains isolated from the patients studied was s1m1cagA+, which was more common among patients with gastric ulcer, duodenal ulcer and gastric cancer. A significant association was observed between the s1m1cagA+ genotype and a higher degree of inflammation, higher neutrophil activity and the development of intestinal metaplasia. The gastric concentrations of IFN-γ and IL-12 were significantly higher in patients infected with H. pylori than in uninfected individuals. Higher levels of these cytokines were detected in patients with gastric ulcer and cancer, while the levels of IL-4 and IL-10 in the gastric mucosa were lower in these patients. In addition, IFN-γ and IL-12 concentrations in gastric biopsies were higher in patients infected with the virulent s1m1cagA+ genotype. In contrast, IL-4 and IL-10 levels were higher in tissue infected with s2m2cagA in gastric biopsies. Conclusion: Our study shows that the interaction between the type of infectious strain and the Th1 immune response can influence and perpetuate gastric inflammation, and thus contributes to the development of the different clinical manifestations of H. pylori infection.
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Hepatitis B and C virus infection among Brazilian Amazon riparians
(2011-10) OLIVEIRA, Claudia Suellen Ferro de; SILVA, Adenielson Vilar e; SANTOS, Kemper Nunes dos; FECURY, Amanda Alves; ALMEIDA, Marcella Kelly Costa de; FERNANDES, Adriana Prado; COSTA, Carlos Araújo da; FREITAS, Andrei Silva; CORVELO, Tereza Cristina de Oliveira; MARTINS, Luisa Caricio
INTRODUCTION: Viral hepatitis is a major public health concern in Brazil. There are few past studies on this issue, especially among riparian communities. This study aims at determining the seroprevalence of viral hepatitis B and C in the riparian community of Pacuí Island, within the Cametá municipality of Pará State, Brazil. Moreover, this study aims to investigate the principal risk factors that this community is exposed to. METHODS: The current study has accessed blood samples from 181 volunteers who have answered an epidemiological questionnaire. Analyses on serological markers have been tested with commercial ELISA kits for detecting HBsAg, total anti-HBc, anti-HBs, and anti-HCV. Within seroreactive patients for HCV, RT-PCR and line probe assay have been performed to identify the viral genotype. RESULTS: In the serological marker analysis for hepatitis B, no reactivity for HBsAg, rate of 1.1% for total anti-HBc, and rate of 19.3% for anti-HBs have been observed. On hepatitis C, 8.8% seroprevalence has been found, in which 62.5% have gotten viral RNA. Among the risk factors studied, the following have been highlighted: non-use of condoms, sharing of cutting instruments, use of illicit drugs, and reports of family disease with HBV or HCV. CONCLUSIONS: The vaccination coverage against HBV is low, and the high prevalence of HCV within this community has been observed.
Acesso aberto (Open Access)
Vírus da Hepatite C: prevalência dos genótipos, fatores de risco, alterações bioquímicas e histopatológicas de pacientes atendidos no Núcleo de Medicina Tropical
(Universidade Federal do Pará, 2011) FECURY, Amanda Alves; MARTINS, Luisa Caricio; http://lattes.cnpq.br/1799493244439769
The hepatitis caused by HCV is a silent disease that tends to develop into the chronic form. The viral persistence depends on genetic factors of the individual and the virus (genotypes), lifestyle and exposure to risk factors increase the chances of the carrier to develop hepatocellular carcinoma. This study objective to check the liver function of patients with hepatitis C and evaluate the risk factors for acquiring the virus and determine the most prevalent HCV genotypes and the genotypes correlate with histopathologic findings of liver biopsies.. The sample consisted of 152 adult patients with reagent serology (ELISA) for anti-HCV, who agreed to participate, collected blood samples for analysis and answered an epidemiological questionnaire. Epidemiological analysis showed most male, age 45 years and a predominance of married or stable individuals. Regarding risk factors for acquiring the infection, there was a multiplicity of partners, not condom use, hospitalizations, and manicure kits share. In the detection of viral RNA, 107 (70,4%) were positive, with 97 (90,6%) genotype 1 and 10 (9,4%) genotype 3. There was no variation in the biochemical assays, the genotypes and histophatological changes. Of the 65 patients who underwent liver biopsy and histopathological examination, all patients had chronic hepatitis. Analyzing the histopatological changes and viral genotypes found association of these variables, with the genotype 1 related histological changes more intense. The results are in agreement with other previously reported.