Navegando por Autor "FEITOSA, Rosimar Neris Martins"

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Acesso aberto (Open Access)
Associação entre marcadores da resposta inflamatória e a imunopatogênese de agentes infecciosos de natureza viral (Vírus da dengue, HTLV-1 e HTLV-2) e bacteriana (Chlamydia trachomatis e Chlamydia pneumoniae)
(Universidade Federal do Pará, 2010-11-29) FEITOSA, Rosimar Neris Martins; ISHAK, Ricardo; http://lattes.cnpq.br/5621101706909450
The genetic basis of diseases is frequently studied aiming the polymorphisms of cytocine genes. The present study investigated markers of the inflammatory response associated to the course of infection and disease caused by viruses and bacteria. Serum levels (measured by an ELISA assay) and the polymorphisms (using PCR, RFLP and allele specific PCR) of TNF-α (-308), TNF-β (+252), IFN-γ (+874) and C reactive protein were measured among persons with febrile disease, infected by dengue virus (n=80), not infected by DV (100), a group of HTLV infected (30 symptomatic and 47 asymptomatic), a group with coronary disease (58 seroreactive to Chlamydia and 31 with negative serology) and a control group (99 persons with no reaction to DV, HTLV and Chlamydia). No group showed association with demographic informations. Dengue virus 3 (66.2%) and HTLV-1 (90% symptomatic and 76.6% asymptomatic persons) were the most frequent agents found among their groups. The majority of those with coronary disease (65.1%) presented antibodies to Chlamydia (39.6% to C. trachomatis and C. pneumoniae, 58.6% solely to C. trachomatis and 1.7% to C. pneumoniae). Statistically significant levels of differences were found among: (i) serum levels of TNF-β, IFN-γ and PrtCR of positive and negative dengue and control groups (p< 0,01); (ii) serum levels of TNF-α, TNF-β and IFN-γ of HTLV (including its types) and control groups; (iii) serum levels of TNF-α, TNF-β, IFN-γ and PrtCR among patients with coronary disease, serum reactive to Chlamydia, and the control group; (iv) the presence of antibodies to C. trachomatis and C. pneumoniae and the control group comparing TNF-β, IFN-γ and PrtCR. Genotypic frequency distributions were statistically significant for the polymorphisms: (i) of TNF-α (p=0,0494) and IFN-γ (p= 0,0008) genes among positive, negative and control dengue groups and to IFN-γ (p= 0,0007) among groups DEN 1, DEN 2, DEN 3 and controls; (ii) of IFN-γ gene (p= 0,0023) among the group of patients with coronary disease and sero reactivity to C. trachomatis e C. pneumoniae, as well as to the mono reactants in the comparison between the positivity to C. trachomatis and the control group.
Acesso aberto (Open Access)
Lack of evidence for human infection with Xenotropic murine leukemia virus-related virus in the Brazilian Amazon basin
(2014-06) GOMES, Samara Tatielle Monteiro; IMBIRIBA, Luciana; BURBANO, Rommel Mario Rodriguéz; SILVA, Artur Luiz da Costa da; FEITOSA, Rosimar Neris Martins; VALLINOTO, Izaura Maria Vieira Cayres; ISHAK, Marluísa de Oliveira Guimarães; ISHAK, Ricardo; VALLINOTO, Antonio Carlos Rosário
Introduction: This study confirmed the absence of natural infection with Xenotropic murine leukemia virus-related virus (XMRV) or XMRV-related disease in human populations of the Brazilian Amazon basin. We demonstrated that 803 individuals of both sexes, who were residents of Belem in the Brazilian State of Pará, were not infected with XMRV. Methods: Individuals were divided into 4 subgroups: healthy individuals, individuals infected with human immunodeficiency virus, type 1 (HIV-1), individuals infected with human T-lymphotrophic virus, types 1 or 2 (HTLV-1/2), and individuals with prostate cancer. XMRV infection was investigated by nested PCR to detect the viral gag gene and by quantitative PCR to detect pol. Results: There was no amplification of either gag or pol segments from XRMV in any of the samples examined. Conclusions: This study supports the conclusions of the studies that eventually led to the retraction of the original study reporting the association between XMRV and human diseases.
Acesso aberto (Open Access)
Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
(Universidade Federal do Pará, 2016-12) QUEIROZ, Maria Alice Freitas; GOMES, Samara Tatielle Monteiro; ALMEIDA, Núbia Caroline Costa de; SOUSA, Maria Izete Machado de; COSTA, Suzanne Roberta Cardoso Fernandes; HERMES, Renata Bezerra; LIMA, Sandra Souza; ZANINOTTO, Marcelo Martins; FOSSA, Marco Antonio Ayin ; MANESCYH, Manoel Araujo; FEITOSA, Rosimar Neris Martins; AZEVEDO, Vânia Nakauth; MACHADO, Luiz Fernando Almeida; ISHAK, Marluísa de Oliveira Guimarães; ISHAK, Ricardo; VALLINOTO, Antonio Carlos Rosário
The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.
Acesso aberto (Open Access)
Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection
(Universidade Federal do Pará, 2015-04) SÁ, Keyla Santos Guedes de; PIRES NETO, Orlando de Souza; SANTANA, Bárbara Brasil; GOMES, Samara Tatielle Monteiro; AMORAS, Ednelza da Silva Graça; CONDE, Simone Regina Souza da Silva; DEMACHKI, Sâmia; AZEVEDO, Vânia Nakauth; MACHADO, Luiz Fernando Almeida; FEITOSA, Rosimar Neris Martins; ISHAK, Marluísa de Oliveira Guimarães; ISHAK, Ricardo; VALLINOTO, Antonio Carlos Rosário
Introduction: The present study investigated the prevalence of two single-nucleotide polymorphisms (SNPs) in the Toll-like receptor 3 (TLR3) gene in patients infected with hepatitis B virus (HBV) and hepatitis C virus (HCV). Methods: Samples collected from HCV (n = 74) and HBV (n = 35) carriers were subjected to quantitative real-time PCR (qPCR) to detect the presence of the SNPs rs5743305 and rs3775291 in TLR3 and to measure the following biomarkers: alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), and prothrombin time (PT). A healthy control group was investigated and consisted of 299 HCV- and HBV-seronegative individuals. Results: No signifi cant differences in allele, genotype and haplotype frequencies were observed between the investigated groups, and no association was observed between the polymorphisms and histopathological results. Nevertheless, genotypes TA/AA (rs5743305) and GG (rs3775291) appear to be associated with higher levels of ALT (p<0.01), AST (p<0.05) and PT (p<0.05). In addition, genotypes TT (rs5743305; p<0.05) and GG (rs3775291; p<0.05) were associated with higher GGT levels. Conclusions: This genetic analysis revealed the absence of an association between the polymorphisms investigated and susceptibility to HBV and HCV infection; however, these polymorphisms might be associated with a greater degree of biliary damage during the course of HCV infection.