Navegando por Autor "HERNANDEZ, Juliana das Merces"

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Sapoviruses in children with acute gastroenteritis from Manaus, Amazon region, Brazil, 2010-2011
(Universidade Federal do Pará, 2016-11-03) REYMÃO, Tammy Kathlyn Amaral; HERNANDEZ, Juliana das Merces; COSTA, Samya Thalita Picanço da; SOUSA, Maisa Silva de; OLIVEIRA, Darleise de Souza; SILVA, Luciana Damascena da; BANDEIRA, Renato da Silva; LIMA, Ian Carlos Gomes de; SOARES, Luana da Silva; MASCARENHAS, Joana D'Arc Pereira; GABBAY, Yvone Benchimol
Sapoviruses (SaVs) are responsible for acute gastroenteritis in humans, especially children and the elderly. In Brazil, data on SaVs infections are very limited, especially in Northern Brazil. Here, we investigated the occurrence of SaVs in samples from hospitalized children under ten years old that presented acute gastroenteritis. Positive samples were genotyped and phylogenetic analysis was performed using prototype strains sequences obtained from GenBank database. In total, 156 fecal samples were screened by RT-PCR for SaVs. A positivity rate of 3.8% (6/156) was found in children under three years of age. Four genotypes were detected: GI.I, GI.2 and GII.2?-GII.4?/GII.4, suggesting a possible inter-genotypes recombination. Most infections (83.3%) occurred between August and September. The positivity was similar to that found in other countries and genotyping demonstrated the presence of distinct genotypes. To our knowledge, this is the first study reporting the circulation of SaVs in Manaus, state of Amazonas, Amazon region, Brazil.