Navegando por Autor "KUMAR, Anil"

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Acesso aberto (Open Access)
Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds
(Public Library of Science, 2013-01) BARH, Debmalya; SHARMA, Neha Barve; GUPTA, Krishnakant; CHANDRA, Sudha; JAIN, Neha; TIWARI, Sandeep; LEON-SICAIROS, Nidia; CANIZALEZ-ROMAN, Adrian; SANTOS, Anderson Rodrigues dos; HASSAN, Syed Shah; ALMEIDA, Sintia Silva de; RAMOS, Rommel Thiago Juca; ABREU, Vinicius Augusto Carvalho de; CARNEIRO, Adriana Ribeiro; SOARES, Siomar de Castro; CASTRO, Thiago Luiz de Paula; MIYOSHI, Anderson; SILVA, Artur Luiz da Costa da; KUMAR, Anil; NARAYAN MISRA, Amarendra; BLUM, Kenneth; BRAVERMAN, Eric R.; AZEVEDO, Vasco Ariston de Carvalho
Vibrio cholerae is the causal organism of the cholera epidemic, which is mostly prevalent in developing and underdeveloped countries. However, incidences of cholera in developed countries are also alarming. Because of the emergence of new drug resistant strains, even though several generic drugs and vaccines have been developed over time, Vibrio infections remain a global health problem that appeals for the development of novel drugs and vaccines against the pathogen. Here, applying comparative proteomic and reverse vaccinology approaches to the exoproteome and secretome of the pathogen, we have identified three candidate targets (ompU, uppP and yajC) for most of the pathogenic Vibrio strains. Two targets (uppP and yajC) are novel to Vibrio, and two targets (uppP and ompU) can be used to develop both drugs and vaccines (dual targets) against broad spectrum Vibrio serotypes. Using our novel computational approach, we have identified three peptide vaccine candidates that have high potential to induce both B- and T-cell-mediated immune responses from our identified two dual targets. These two targets were modeled and subjected to virtual screening against natural compounds derived from Piper betel. Seven compounds were identified first time from Piper betel to be highly effective to render the function of these targets to identify them as emerging potential drugs against Vibrio. Our preliminary validation suggests that these identified peptide vaccines and betel compounds are highly effective against Vibrio cholerae. Currently we are exhaustively validating these targets, candidate peptide vaccines, and betel derived lead compounds against a number of Vibrio species.
Acesso aberto (Open Access)
Whole-Genome Sequence of Corynebacterium pseudotuberculosis Strain Cp162, Isolated from Camel
(American Society for Microbiology, 2012) HASSAN, Syed Shah; SCHNEIDER, Maria Paula Cruz; RAMOS, Rommel Thiago Juca; CARNEIRO, Adriana Ribeiro; LIMA, Alex Ranieri Jerônimo; GUIMARÃES, Luís Carlos; ALI, Amjad; BAKHTIAR, Syeda Marriam; PEREIRA, Ulisses de Pádua; SANTOS, Anderson Rodrigues dos; SOARES, Siomar de Castro; DORELLA, Fernanda Alves; GOMIDE, Anne Cybelle Pinto; RIBEIRO, Dayana; BARBOSA, Maria Silvanira Ribeiro; ALMEIDA, Sintia Silva de; ABREU, Vinicius Augusto Carvalho de; ABURJAILE, Flavia Figueira; NASCIMENTO, Karina Kelly Fiaux do; BARBOSA, Eudes Guilherme Vieira; DINIZ, Carlos Augusto Almeida; ROCHA, Flávia de Souza; SAXENA, Rashmi; TIWARI, Sandeep; ZAMBARE, Vasudeo; GHOSH, Preetam; PACHECO, Luis Gustavo Carvalho; DOWSON, Christopher G.; KUMAR, Anil; BARH, Debmalya; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de Carvalho; SILVA, Artur Luiz da Costa da
Corynebacterium pseudotuberculosis is a pathogen of great veterinary and economic importance, since it affects livestock, mainly sheep and goats, worldwide, together with reports of its presence in camels in several Arabic, Asiatic, and East and West African countries, as well as Australia. In this article, we report the genome sequence of Corynebacterium pseudotuberculosis strain Cp162, collected from the external neck abscess of a camel in the United Kingdom.