Navegando por Autor "LAGE, Andrey Pereira"

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Acesso aberto (Open Access)
Complete Genome Sequence of Type Strain Campylobacter fetus subsp. venerealis NCTC 10354
(American Society for Microbiology, 2011-10) STYNEN, Ana Paula Reinato; LAGE, Andrey Pereira; MOORE, Robert J.; REZENDE, Antonio Mauro; FERREIRA, Vívian D'Afonseca da Silva; RUY, Patrícia de Cássia; OLIVEIRA, Nesley Jesus Daher de; RESENDE, Daniela de Melo; ALMEIDA, Sintia Silva de; SOARES, Siomar de Castro; ABREU, Vinicius Augusto Carvalho de; ROCHA, Aryane Aparecida Magalhães Cassiano; SANTOS, Anderson Rodrigues dos; BARBOSA, Eudes Guilherme Vieira; COUTO, Danielle Costa Carrara; DORELLA, Fernanda Alves; MIYOSHI, Anderson; LIMA, Alex Ranieri Jerônimo; CAMPOS, Frederico Davi da Silva; SÁ, Pablo Henrique Caracciolo Gomes de; LOPES, Thiago Souza; RODRIGUES, Ryan Maurício Araújo; CARNEIRO, Adriana Ribeiro; LEÃO, Tiago Ferreira; CERDEIRA, Louise Teixeira; RAMOS, Rommel Thiago Juca; SILVA, Artur Luiz da Costa da; AZEVEDO, Vasco Ariston de Carvalho; RUIZ, Jeronimo Conceição
Campylobacter fetus subsp. venerealis is the etiologic agent of bovine genital campylobacteriosis, a sexually transmitted disease of cattle that is of worldwide importance. The complete sequencing and annotation of the genome of the type strain C. fetus subsp. venerealis NCTC 10354T are reported.
Acesso aberto (Open Access)
Quadruplex PCR assay for identification of Corynebacterium pseudotuberculosis differentiating biovar Ovis and Equi
(BioMed Central Ltd, 2017) ALMEIDA, Sintia Silva de; DORNELES, Elaine Maria Seles; DINIZ, Carlos Augusto Almeida; ABREU, Vinicius Augusto Carvalho de; SOUSA, Cassiana Severiano de; ALVES, Jorianne Thyeska Castro; CARNEIRO, Adriana Ribeiro; VILAS BOAS, Priscilla Carolinne Bagano; SPIER, Sharon; BARH, Debmalya; LAGE, Andrey Pereira; FIGUEIREDO, Henrique Cesar Pereira; AZEVEDO, Vasco Ariston de Carvalho
Background: Corynebacterium pseudotuberculosis is classified into two biovars, nitrate-negative biovar Ovis which is the etiologic agent of caseous lymphadenitis in small ruminants and nitrate-positive biovar Equi, which causes abscesses and ulcerative lymphangitis in equines. The aim of this study was to develop a quadruplex PCR assay that would allow simultaneous detection and biovar-typing of C. pseudotuberculosis. Methods: In the present study, genomes of C. pseudotuberculosis strains were used to identify the genes involved in the nitrate reduction pathway to improve a species identification three-primer multiplex PCR assay. The nitrate reductase gene (narG) was included in the PCR assay along with the 16S, rpoB and pld genes to enhance the diagnosis of the multiplex PCR at biovar level. Results: A novel quadruplex PCR assay for C. pseudotuberculosis species and biovar identification was developed. The results of the quadruplex PCR of 348 strains, 346 previously well-characterized clinical isolates of C. pseudotuberculosis from different hosts (goats, sheep, horse, cattle, buffalo, llamas and humans), the vaccine strain 1002 and the type strain ATCC 19410T , were compared to the results of nitrate reductase identification by biochemical test. The McNemar’s Chi-squared test used to compare the two methods used for C. pseudotuberculosis biovar identification showed no significant difference (P = 0.75) [95% CI for odds ratio (0.16–6.14)] between the quadruplex PCR and the nitrate biochemical test. Concordant results were observed for 97.13% (338 / 348) of the tested strains and the kappa value was 0.94 [95% CI (0.90–0.98)]. Conclusions: The ability of the quadruplex assay to discriminate between C. pseudotuberculosis biovar Ovis and Equi strains enhances its usefulness in the clinical microbiology laboratory.