Navegando por Autor "MARTINOTTO, Cristiano"
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Item Acesso aberto (Open Access) Efeito da escarificação e luminosidade na germinação in vitro de sementes de cagaiteira (Eugenia dysenterica DC.)(2007-12) MARTINOTTO, Cristiano; PAIVA, Renato; SANTOS, Breno Régis; SOARES, Fernanda Pereira; HERRERA, Raírys Cravo; SILVA, Álvaro Augusto NavesCagaiteira is a native specie of brazilian cerrado with high yield. Because it seeds show high variability, it is yet considered recalcitrant and dormant, the germination in vitro appears as an alternative for specie propagation, in addition to obtain juvenile explant for micropropagation. The present research aimed to evaluate the effect of scarification and ligth on germination in vitro of cagaiteira seeds. Seeds without teguments and intact seeds were inoculated in MS medium and kept in abscence and presence of radiation. Seeds without tegument germinated in the presence or abscence of ligth showed 86.25% and 88.25% germination at 31 and 71 days of inoculation, respectively. After 150 days of inoculation, plantlets from seeds without tegument germinated in the presence and abscence of ligth, showed 10% and 12% of abnormalities, respectively.Item Acesso aberto (Open Access) Morfogênese in vitro de nim a partir de explantes cotiledonares(2009) RODRIGUES, Marcelo; PAIVA, Renato; HERRERA, Raírys Cravo; MARTINOTTO, Cristiano; SILVA JÚNIOR, Jessé MarquesAzadirachta indica A. Juss, popularly known as neem, is a woody species widely used because of its insecticide, fungicide, bactericide, and nematicide properties. Seeds of neem were inoculated in WPM (Wood Plant Medium) containing different concentrations of gibberelic acid (GA3) (0, 3, 6, 9, and 12 mg L-1). After 30 days, cotyledons were inoculated in WPM supplemented with 0.0; 1.0; 2.0 and 3.0 mg L-1 acid 2,4-phenoxyacetic acid (2,4-D) and/or 0.0; 1.0; 2.0 and 3.0 mg L-1 6-benzylaminopurine (BAP). The cultures were kept in the dark at 28 ºC. Differentiated calli were evaluated based on their coloration and texture, and three subcultures were carried out on a monthly basis in WPM medium with 2.0 mg L-1 BAP, in the presence of light. Every 30 days, the number of differentiated shoot-buds was evaluated. WPM medium lacking GA3 promoted the highest in vitro seed germination indexes. Cotyledonary-derived explants cultured in WPM medium with 2.0 mg L-1 BAP yielded calli with high morphogenic potential. When those calli were transferred to medium with similar composition, a high shoot formation was achieved up to the third subculture.