Navegando por Autor "MEDEIROS, Arnaldo Correia de"
Agora exibindo 1 - 2 de 2
- Resultados por página
- Opções de Ordenação
Item Acesso aberto (Open Access) Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic(Universidade Federal do Pará, 2015-06) LIMA, Eleonidas Moura; LOPES, Otávio Sérgio; SOARES, Leonardo Ferreira; ARRUDA, Talitta Dantas; GIGEK, Carolina Oliveira; MELO, Cynthia Germoglio Farias; SMITH, Marília de Arruda Cardoso; OLIVEIRA, João Ricardo Gonçalves; MEDEIROS, Arnaldo Correia de; DELATORRE, Plínio; BURBANO, Rommel Mario RodriguézGastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ 2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ 2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.Item Acesso aberto (Open Access) Investigation of single-strand conformational polymorphism of the TP53 gene in women with a family history of breast cancer(2000-11) BURBANO, Rommel Mario Rodriguéz; MEDEIROS, Arnaldo Correia de; MELLO, Adriano Azevedo de; LEMOS, José Alexandre Rodrigues de; BAHIA, Marcelo de Oliveira; CASARTELLI, CacildaBreast cancer in families with germ line mutations in the TP53 gene has been described in the medical literature. Mutation screening for susceptibility genes should allow effective prophylactic and preventive measures. Using single-strand conformational polymorphism, we screened for mutations in exons 5, 6, 7 and 8 of gene TP53 in the peripheral blood of 8 young non-affected members (17 to 36 years old) of families with a history of breast cancer. Studies of this type on young patients (mean age, 25 years) are very rare in the literature. The identification of these mutations would contribute to genetic counseling of members of families with predisposition to breast cancer. The results obtained did not show any polymorphism indicating mutation. In our sample, the familial tumorigenesis is probably related to other gene etiologies.