Navegando por Autor "MORAES, Carina Martins de"

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Acesso aberto (Open Access)
Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil
(Universidade Federal do Pará, 2016-08) BRITO, Jacqueline da Silva; MORAES, Carina Martins de; SILVA, Cleyzer Lopes; SALES, Gustavo Aguiar; KEID, Lara Borges; MATOS, Paulo Cesar Magalhães; LARA, Ana P.S.S.; MORAES, Carla Cristina Guimarães de
Brucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differentiate between a vaccine strain (B19) and the field strain. Sixty-six samples of different cheeses which are produced and marketed in three states of the Brazilian Amazon region (Amapá [5 samples], Pará [55 samples] and Rondônia [6 samples]) were evaluated. Thirty-nine of these samples were from cheeses made from cow's milk, and 27 were from cheeses made from buffalo milk. Four of the 66 samples were from cheeses produced in milk processing plants regulated by the Federal Inspection Service (Serviço de Inspeção Federal); nine of the samples were from cheeses produced in processing plants regulated by the State Inspection Service (Serviço de Inspeção Estadual); five of the samples were from artisanal cheeses; and the remaining 48 samples were from informally produced cheese. DNA was obtained from the samples following a DNA extraction protocol, and PCR was conducted using primers B4 and B5 to detect Brucella spp. Primers eri1 and eri2 were used to differentiate the field strain from the B19 vaccine strain. The results showed that 21.21% (14/66) of the samples were positive for Brucella spp., of which 21.43% (3/14) were positive for the B. abortus field strain, and 7.14% (1/14) were identified as harboring vaccine strain B19. These results demonstrate that it is possible to identify Brucella spp. in cheese from the Amazon region using the PCR technique and to differentiate the B. abortus field strain from the B19 vaccine strain.
Acesso aberto (Open Access)
Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA
(2014-08) MORAES, Carina Martins de; CONCEIÇÃO, Fabrício Rochedo; ROCHA, Andréa da Silva Ramos; SANTOS JÚNIOR, Alceu Gonçalves dos; RIBAS, Leandro dos Montes; VARGAS, Agueda Palmira Castagna de; NOGUEIRA, Carlos Eduardo Wayne; TURNES, Carlos Gil; LEITE, Fabio Pereira Leivas
Strangles is an economically important horse disease caused by Streptococcus equi subsp. equi. The diagnosis can be confirmed either directly by bacterial isolation and PCR or by ELISA, which is an indirect method based on the detection of serum antibodies. The aim of this study was to clone, express and characterize the SeM protein of Streptococcus equi subsp. equi, evaluate its use as antigen in indirect ELISA and determine its performance to distinguish sera of negative, vaccinated and positive animals. This was initially performed by cloning the gene encoding the SeM protein and its expression in Escherichia coli. Subsequently, the protein produced was characterized and used as antigen in ELISA. Serum samples for evaluation were taken from 40 negative foals, 46 horses vaccinated with a commercial vaccine against strangles and 46 horses diagnosed with the disease. The test showed high specificity and sensitivity, allowing discrimination between negative and positive, positive and vaccinated animals, and vaccinated animals and negative sera. Thus, it was concluded that the protein produced rSeM, which can be used as antigen for disease diagnosis, and the described ELISA might be helpful to evaluate the immune status of the herd.
Acesso aberto (Open Access)
Palm kernel meal as additive in the elephant-grass silage
(Universidade Federal do Pará, 2014-09) SANTOS, Ivan Alberto Palheta; DOMINGUES, Felipe Nogueira; RÊGO, Aníbal Coutinho do; SOUZA, Natália Sidrim da Silva de; BERNARDES, Thiago Fernandes; BARATA, Zuleide Rafaela Pimentel; MORAES, Carina Martins de
The objective of this study was to evaluate the effect of adding palm kernel meal (BP) as an additive in elephant grass silage. The chemical qualitative and fermentation were analysed in a completely randomized design with six treatments, one with no BP (C) and six replications, where the averages of the results of days of closing and opening were analysed by regression analysis. We collected samples at the time of ensiling and after 190 days of storage for evaluation of dry matter, crude protein, carbohydrates (total, non-fibrous, water-soluble, cellulose and hemicellulose), ash, lignin and ether extract. In aerobic phase, we collected samples for pH, ammoniacal nitrogen, fungi and yeasts. The elephant grass of this experiment showed values of 13.9% DM and 8.25% CP while the BP has 77.2% DM and 16.9% CP. The inclusion of BP had inhibited the growth of fungi and yeasts. The increase in the content of BP decreased the concentration of NH3-N. Higher concentrations of BP in silage were more stable, and the stability breaks in 72 hours, while the stability of C was 19 hours. Concentrations above 15% BP had no significant variations in pH in the aerobic phase. The inclusion of palm kernel meal at concentrations 10% to 15% can be used in silage of elephant grass, inhibiting undesired fermentation and making them more stable. Concentrations above this value may affect the nutritive value of silage by high lignin content of the additive.
Acesso aberto (Open Access)
Segmentation of the buffalo meat consumer market in Belém, Pará, Brazil
(Universidade Federal do Pará, 2016-06) MARQUES, Cristiane Soares Simon; OAIGEN, Ricardo Pedroso; MORAES, Carina Martins de; SANTOS, Marcos Antônio Souza dos; LOURENÇO JÚNIOR, José de Brito; BEZERRA, Isis Abel
The objective of this study was to identify different market segments for buffalo meat with target groups defined in the city of Belém, Pará, Brazil. Paired samples of beef and buffalo meat were given to 447 volunteers, and a sensory analysis of the products was conducted. After the tasting, a questionnaire was administered to obtain demographic (age, educational level, marital status) and economic (household income) data as well as information on the habits of respondents and preferences regarding buffalo meat. The factors were estimated using the principal components method and the factors with characteristic roots greater than one were extracted. To check the suitability of the factorial model, Bartlett’s sphericity test and the Kaiser-Meyer-Olkin (KMO) test were used. A factor analysis was performed, identifying five factors with common variability dimensions. The study has helped identify four distinct market segments which, combined with a targeted marketing strategy, can be used to leverage the productive chain: I - Young and interested, II - Mixed and indifferent, III - Graduates and successful, and IV - Healthy women. All had positive responses to buffalo meat; clusters I and III stood out with regard to several indicators, especially those related to the preference for buffalo meat and the predisposition to purchase and include it in meals. The sensory analysis indicated that consumers showed a greater acceptance of buffalo meat based on the characteristics of flavour, colour, succulence and tenderness. The study helps to identify market segments with distinct features that, combined with a targeted marketing strategy, can be used to leverage the supply chain.