Navegando por Autor "NOBRE, Akim Felipe Santos"

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Acesso aberto (Open Access)
Diversidade genética dos vírus linfotrópicos de Células T do tipo 1 na Região Metropolitana de Belém
(Universidade Federal do Pará, 2015) NOBRE, Akim Felipe Santos; SOUSA, Maísa Silva de; http://lattes.cnpq.br/1775363180781218; SOUSA, Rita Catarina Medeiros; http://lattes.cnpq.br/3560941703812539
The human T-cell lymphotropic virus type 1 (HTLV-1) is a DELTARETROVIRUS which was first isolated from a blood sample of a patient with african american cutaneous T-cell lymphoma, in the mid 1970s; Hodgkin was later classified as leukemia / lymphoma adult T-cell (LLcTA) severe disease that affects T lymphocytes Subsequently, the virus was also associated with tropical spastic paraparesis / HTLV-associated myelopathy (HAM / TSP), character chronic and progressive causing damage mainly at the thoracic spinal cord. Their genetic diversity varies according to the region studied, and its mutation rate is very low (about 1% per thousand years) compared to other viruses. The objective of this study was to assess the genetic diversity of HTLV-1 in the metropolitan area of Belém, as well as the frequency of genotypes and the comparison of samples obtained with the sequences available in databases such as GenBank. We used blood samples collected from patients enrolled in the NMT / UFPA between the period January 2010 to December 2013. Then these samples were subjected to DNA extraction and amplification of HTLV PX region, using oligonucleotides inciciadores specific, from the PCR in two steps: internal and external (nested PCR). Positive samples then were subjected to enzymatic digestion of TaqI enzyme so that we could identify and differentiate the HTLV 1 and 2. Next, we performed amplification of the 5 'LTR region also by nested PCR positive samples, which, then were subjected to genetic sequencing. Using the method of maximum likelihood, we constructed a phylogenetic tree for the group display in clades of sequences in question. A Bayesian analysis (molecular clock) to visualize the evolutionary profile of the samples was also performed. The tests identified 78 samples positive for HTLV-1, of these, 44 were sequenced. The aa genotype was common in 100% of samples; the different subtypes had the following range rate and mutations per site per year: a- 2.10 -3; b- 2,69 . 10 -2; c- 6,23 . 10 -2; d- 3,08 . 10 -2; e- 6 . 10 -2; f- 1,78 . 10 -3; g- 2,2 . 10 -2 changes per site per year. It was then revealed a low genotypic diversity and a high genetic stability of the samples positive for HTLV-1 in the region.
Acesso aberto (Open Access)
Hematological changes in human lymphotropic-T virus type 1 carriers
(Frontiers Media S.A., 2022) RIBEIRO, Jairo Falcão; NOBRE, Akim Felipe Santos; COVRE, Louise Canto Ferreira; VIANA, Maria de Nazaré do Socorro de Almeida; SILVA, Ingrid Christiane; SANTOS, Leonardo Miranda dos; ISHIKAWA, Edna Aoba Yassui; COSTA, Carlos Araújo da; SOUSA, Maísa Silva de
The human T-lymphotropic virus type 1 (HTLV-1), isolated in 1980, causes T-cell leukemia/lymphoma in adulthood, a type of lymphoproliferative disease, and chronic HTLV-1-associated myelopathy, a disease that causes paralysis of the lower limbs, which occur in about 5% of cases in this viral infection. This study aimed to establish the hematological profile of patients with HTLV-1 infection in Belém do Pará, describing the hematological parameters under study, estimating the frequency of lymphocytic atypical, and associating the hematological profile with diseases and symptoms. Hematologic data from 202 individuals were analyzed, including 87 HTLV-1 infected individuals and 115 non-HTLV-1 infected individuals as a control group, composed, at a great part, of relatives of the infected. The seroprevalence of HTLV-1 infection was observed in 71.3% of female individuals, with predominance in the group older than 50 years (44.8%). The analysis of hematological parameters showed a significant difference in the counts of the segmented cells (p = 0.0303) and eosinophils (p = 0.0092) in HTLV-1 carriers. Lymphocytic atypical was a finding present only in HTLV-1 carriers (p = 0.0001). There was no high frequency in the leukocyte counts of those infected by HTLV-1 not among them concerning a significant increase or decrease. It is concluded that HTLV-1 infection is prominent in women over 50 years old. The hematological profile of those infected shows a reduction of segmented cells, an increase of eosinophils, and the presence of atypical lymphocytes. The hematological profile of the HTLV-1 carrier should always be evaluated to identify early some diseases associated with the infection.
Acesso aberto (Open Access)
Moderada endemicidade da infecção pelo vírus linfotrópico-T humano na região metropolitana de Belém, Pará, Brasil
(Universidade Federal do Pará, 2018-10) SILVA, Ingrid Christiane; PINHEIRO, Bruna Teles; NOBRE, Akim Felipe Santos; COELHO, Jaciana Lima; PEREIRA, Cássia Cristine Costa; COVRE, Louise de Souza Canto; ALMEIDA, Camila Pâmela Santos de; VIANA, Maria de Nazaré do Socorro de Almeida; ALMEIDA, Danilo de Souza; RIBEIRO, Jairo Falcão; SANTOS, Yago Costa Vasconcelos dos; ARAÚJO, Marcos William Leão de; BORGES, Mariza da Silva; NASCIMENTO, Lisandra Duarte; VALENTIM, Lorena Saldanha; CASSEB, Jorge Simao do Rosario; COSTA, Carlos Araujo da; SOUSA, Maisa Silva de
The spread of the HTLV infection in families living in the metropolitan area of Belém, Pará, Brazil, and the lack of studies in the general population requires studies to better understand its prevalence in the region. Methods: An anti-HTLV-1/HTLV-2 antibodies test was carried out on random adults in public places in Belém between November 2014 and November 2015. A proviral DNA test detected if the person was infected, and then a clinical evaluation and an intrafamilial investigation were carried out. Results: Of the 1059 individuals being investigated, 21 (2.0%) had seroreagent samples, 15 (1.4%) had HTLV-1, 5 (0.5%) had HTLV-2, and proviral DNA was undetectable in one case. The mean age of the infected people (57.2) was higher than that of those that were uninfected (46.2) (p = 0.0010). The prevalence of infection increased with age, especially in individuals with a family income equal to or less than a minimum wage. Intrafamilial transmission seems to have occurred in all of the families being studied. Among the patients with HTLV-1, 30% (3/10) already had some symptom related to the infection. Discussion: The increase in prevalence rates according to age may be due to late seroconversion of a previously acquired infection, or the cumulative risk of new infections, especially in women. Conclusion: There was a moderate prevalence of the HTLV infection among adult individuals from the metropolitan area of Belém, with a predominance of HTLV-1. This infection was associated with low income and increasingly older women. It also presented intrafamily spread and negligence in the diagnosis of associated diseases.