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Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
(Universidade Federal do Pará, 2018-02) QUEIROZ, Sabrina Ribeiro de Almeida; SILVA JÚNIOR, José Valter Joaquim; SILVA, Andrea Nazare Monteiro Rangel da; OLIVEIRA, Amanda Gomes de; SANTOS, Jefferson José da Silva; GIL, Laura Helena Vega Gonzales
Introduction: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. Methods: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. Results: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. Conclusions: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.