Navegando por Autor "PONTES, Ana Rosa Botelho"

Agora exibindo 1 - 3 de 3

Acesso aberto (Open Access)
Detecção de Mycobacterium lepra por PCR em "SWAB" nasal e "SWAB" da linfa do lóbulo da orelha de pacientes hansenianos
(Universidade Federal do Pará, 2007-11-30) PONTES, Ana Rosa Botelho; ISHIKAWA, Edna Aoba Yassui; http://lattes.cnpq.br/3074963539505872
Recently some studies have used Polymerase Chain Reaction (PCR) technique for detection of the Mycobacterium leprae DNA, in diverse biological samples, demonstrating high sensitivity. The objective of this work was to evaluate the sensitivity of the PCR in the detection of M. leprae in nasal swab and lymph swab from ear lobe of leprosy patients and to compare the results of the PCR with the bacilloscopy and histophatology and multibacillary and paucibacillary forms of leprosy. Nasal secretion samples and lymph of the lobe of the ear of 24 leprosy patients had been collected. For amplification of the DNA three pairs of primers had been tested: S13 and S62, R1 and R2, LP1 and LP2 that amplify fragments of 531 DNA of pb, 372pb and 129pb, respectively. The primers LP1 and LP2 had expressed greater sensitivity, independent of the clinical samples. The results of the PCR had been highly significant for the nasal secretion samples (p<0.0000) and significant for specimens of lymph of the lobe of the ear (p=0.0000). Comparing the results of the PCR, using primers LP1 and LP2 and conservante lise 1, with the bacilloscopy and histophatology, the studies had pointed that the PCR, in nasal secretion samples, got greater sensitivity for the MBs forms (41,67%), followed of the bacilloscopy (25%) and histophatology (8,33%). In the PBs forms, sensitivity was considered same between the PCR and the histophatology (8,33%). The bacilloscopy did not present sensitivity (0%). In the samples of the lymph of the lobe of the ear, the bacilloscopy demonstrated to greater sensitivity for the MBs forms (25%), followed of the PCR (20,83%) and histophatology (16,7%). In the PBs forms, the PCR and histophatology had presented same sensitivity (4,17%). It did not have sensitivity in the bacilloscopy (0%). The PCR, although not to demonstrate a 100% sensitivity it is a tool with future perspectives to assist in the monitoring of the treatment and cure of the leprosy patients.
Acesso aberto (Open Access)
Detecção do DNA de Mycobacterium leprae em secreção nasal
(2008-11) PONTES, Ana Rosa Botelho; ALMEIDA, Maria das Graças Carvalho; XAVIER, Marília Brasil; QUARESMA, Juarez Antônio Simões; ISHIKAWA, Edna Aoba Yassui
Studies have demonstrated high sensibility of the polimerase chain reaction (PCR) technique in the identification of the Mycobacterium leprae DNA . This study aimed to evalue the PCR sensibility at the detection of the M. leprae DNA in nasal swab of leprosy patients and to compare the results with the bacilloscopy and multibacillary (MBs) and paucibacilares (PBs) forms. Nasal secretion samples of 24 leprosy patients were collected, and were preserved in one and two lise's solution. The PCR results were highly significant (p <0.0000) and they revealed grater sensibility than bacilloscopy, in several clinical forms. Nevertheless, still different studies are necessary, testing new markers and preservatives, with the purpose of lifting up the sensibility of this technique, in nasal secretion samples.
Acesso aberto (Open Access)
Uso de técnica de biologia molecular para detecção do Mycobacterium leprae, em combinação com a avaliação dermatoneurológica, no diagnóstico precoce dos contatos intradomiciliares de hanseníase
(Universidade Federal do Pará, 2016-06-28) PONTES, Ana Rosa Botelho; ISHIKAWA, Edna Aoba Yassui; http://lattes.cnpq.br/3074963539505872
The purpose of this study was to apply the technique of molecular biology in nasal secretion sample from household contacts of leprosy patients, in combination with dermatological and neurological assessments, improving the early diagnosis of leprosy. The study was conducted in health municipal units of Belém-PA, from February 2013 to April 2015. The sample consisted of 154 household contacts and 58 index cases of leprosy, totaling 212 subjects. Data collection was through epidemiological record, dermatologic and neurological evaluations and BCG scar examination. A nasal secretion sample from each subject for the PCR was collected. In index cases, positive PCR was in the age group of 40-59 years (35.0%), in multibacillary (MB) form (80,0%); cases with 7 to 9 skin lesions (35.0%) and with no enlarged nerves (40.0%). All positive index PCR cases show signs and symptoms of leprosy (34.5%) and most do not have BCG scar (65.0%). Among the contacts, positive PCR prevailed in females (63.9%); in the age group 20-39 years (44.4%); family income of a minimum wage (47.2%); student occupation (33.3%) and completed high school (36.1%). The strongest evidence of positive PCR in index cases and contacts was in multibacillary, respectively (37.2% and 25.6%). Proves to be a highly significant correlation between clinical signs and symptoms with PCR in nasal secretion of multibacillary contacts, indicating that if they get sick are more likely to reproduce the same operating way of index cases. The highest percentage of positive PCR was in contact with the absence of BCG scar (25.8%). The association between the signs, symptoms and PCR indicates that contacts with positive PCR have 07 times more likely to show signs and symptoms of leprosy. In estimating the potential risk for the development of leprosy in contacts, we identified 22 (14.3%) at intermediate risk and 06 (3.9% 0) at high risk. One hundred and forty-four (144) contacts said that they have daily contact with the index case (93.5%) and of these 36 (25.0%) were positive for PCR. The treatment of index cases led to a reduction of PCR positivity in both modes of operation. The correlation between PCR and the degree of disability of the index cases, grade 1 was more prevalent (55.0%). M. leprae DNA detection in the nasal secretion of household contacts for PCR, in association with dermatological and neurological assessments, raises the effectiveness of early diagnosis of leprosy, contributing to the control of the disease in the community.