Navegando por Autor "QUEIROZ, Marcos Fabiano de Almeida"

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Acesso aberto (Open Access)
Evaluation of agreement between tests for the diagnosis of leprosy
(Sociedade Brasileira de Patologia Clínica, 2017-04) SILVA, Alison Rodrigues da; QUEIROZ, Marcos Fabiano de Almeida; ISHIKAWA, Edna Aoba Yassui; SILVESTRE, Maria do Perpétuo Socorro Amador; XAVIER, Marilia Brasil
Introduction: Leprosy is a chronic infectious disease caused by the intracellular parasite Mycobacterium leprae. The diagnosis is essentially clinical, based on symptoms, skin exam, peripheral nerves and epidemiological history. Laboratory tests are carried out to complement the result of clinical diagnosis, or even serving as a confirmatory method. Objective: To investigate the positivity and agreement between skin smear, enzyme-linked immunosorbent assay (ELISA) with synthetic antigen ND-O-BSA, ML Flow test and polymerase chain reaction (PCR) for detection of Mycobacterium leprae in new cases of leprosy. Methods: We conducted a case series study assessing a convenience sample of 39 new cases of leprosy and a control group of 18 household contacts in Belém (PA) and in Igarapé-Açu (PA) from March 2014 to September 2015. Results: The agreement between ELISA, ML Flow and PCR tests combinations showed slight to absent reproducibility (Kappa ≤ 0.24). The results showed greater sensitivity in PCR assay, with higher positivity in multibacillary cases. The ELISA test showed low positivity, even in multibacillary cases, resulting in no reaction to paucibacillary cases and household contacts. Conclusion: The high sensitivity of PCR decreases the agreement with other tests.
Acesso aberto (Open Access)
Soroprevalência de testes rápidos (ML Flow) em casos de hanseníase e contatos intradomiciliares em municípios endêmicos do Pará
(Universidade Federal do Pará, 2012-05-22) QUEIROZ, Marcos Fabiano de Almeida; XAVIER, Marília Brasil; http://lattes.cnpq.br/0548879430701901
Leprosy, a millennial, still poses a major challenge to public health, including in relation to early diagnosis and health surveillance. In order to determine the seroprevalence of antiPGL-1 through ML-Flow rapid test in leprosy cases and their household contacts of endemic municipalities in Pará, we performed a cross-sectional study including 73 new leprosy cases and 135 household contacts, selected from April 2011 to January 2012, the reference units for treatment of leprosy in the municipalities of Belém, Marituba, Igarapé-Açú and Santarém. The results showed a prevalence of 14.8/10.000 inhabitants of leprosy cases among contacts investigated. The ML Flow seropositivity in index cases was 53.42% in multibacillary and 13.33% in household contacts. There was a direct association of positive ML Flow in household contact with the bacterial index of the index case. There was no association with time of coexistence and consanguinity of the index case in relation to Ml Flow test. There was an association between positive ML Flow test and realization of BCG between the contacts. These results indicate that the introduction of the ML Flow test could serve as an aid in the monitoring of cases and their contacts, becoming very important in epidemiological surveillance of leprosy.