Navegando por Autor "REIS, Adriana Novaes dos"

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Acesso aberto (Open Access)
Efeito do estradiol e da progesterona no desenvolvimento e na qualidade de embriões bovinos produzidos in vitro
(2010-12) REIS, Adriana Novaes dos; SILVA, Lílian Kátia Ximenes; SILVA, Aluizio Otavio Almeida da; SOUSA, José Silva de; VALE, William Gomes
The development and quality of bovine embryos co-cultivated with bovine oviduct epithelial cells (BOEC) supplemented or not with estradiol and progesterone were evaluated. Oocytes were matured and fertilized in vitro (MIV/FIV) (5%CO2/38.5oC). The BOEC were cultivated in TCM-199 with or without estradiol (E2) (24h), in the same conditions of MIV/FIV. Presumptive zygotes were transferred to BOEC in suspension after in vitro maturation and fertilization of bovine oocytes with thawed percoll-selected sperm. The zygotes were cultivated in CR2 medium containing or not progesterone (P4) (G1=P4+E2), (G2=E2), (G3=P4), and (G4=control). No significant differences (P>0.05) were found in the cleavage rates among G1, G2, and G4 (53.5%, 56.3%, and 51.7%) as well as in relation to the blastocystis (BL) rates (29.3%, 31.2%, and 28.7%). However, significant differences (P<0.05) were observed in the G3 for both variables (34.5% and 16.4%). G1 and G2 showed hatching rates higher (P<0.05) than the other groups (23.3%; 23.2%), being G4 (19.3%) different from G3 (16.1%) (P<0.01). In the groups G1, G2, and G3, the total cell number of the BL increased (125.9, 128.4, and 123.6) (P<0.05) compared to G4 (112.5). These results demonstrate that the treatment of the BOEC with estradiol in first the 24 hours of culture can be used separately or in combination with the progesterone to improve the quality of bovine embryos produced in vitro.
Acesso aberto (Open Access)
Transporte de oócitos bovinos em meio de maturação por diferentes períodos de tempo sem controle da atmosfera gasosa
(2011-02) SILVA, Lílian Kátia Ximenes; REIS, Adriana Novaes dos; SILVA, Aluizio Otavio Almeida da; SOUSA, José Silva de; SOUSA, Alysson Jorge de Oliveira; VALE, William Gomes
The viability of the transport of the bovine oocytes was evaluated in chemically defined medium and the need for the addition or not of hormones in this medium was analyzed. The oocytes in the control group (0h) were matured for 24h in CO2 incubator, and in experimental groups they were transported in portable incubator. In experiment I, the cleavage rates were similar (P>0.05) to the groups 0h (59.7%), 3h (53.5%), and 9h (48.8%), but they decreased in groups 6h (46.1%) and 12h (43.8%), however, these rates were similar among the groups 3h, 6h, 9h, and 12h. The production of blastocysts was not different (P>0.05) for groups 0h (38.0%), 3h (32.3%), 6h (27.3%), and 9h (24.8%), but there was a reduction in the 12h group (18.9%). These rates were similar among the groups 6h, 9h and 12h. In experiment II, no significant difference (P>0.05) was observed among the rates of cleavage for the groups 0h (71.4%), 3h with (70.3%) and without hormones (64.8%), and 6h with (56.0%) and without hormones (54.1%). The production of blastocysts was similar (P>0.05) for groups 0h (46.1%) and 3h with (45.8%) and without hormones (41.1%), but decreased in groups 6h with (35.5%) and without hormones (33.5%). These rates were similar among the groups 3h without, 6h with and without hormones. These results indicate the possibility of the transport of bovine oocytes up to 9h, and the addition of hormones in this medium does not influence the rates of cleavage and blastocysts.