Navegando por Autor "REIS, Alessandra dos Santos Belo"

Agora exibindo 1 - 15 de 15

Acesso aberto (Open Access)
Aspectos clínico-patológicos do envenenamento botrópico experimental em equinos
(2011-09) SOUSA, Melina Garcia Saraiva de; TOKARNIA, Carlos Maria Antônio Hubinger; BRITO, Marilene de Farias; REIS, Alessandra dos Santos Belo; OLIVEIRA, Carlos Magno Chaves; FREITAS, Nayra Fernanda de Queiroz Ramos; OLIVEIRA, Cairo Henrique Sousa de; BARBOSA NETO, José Diomedes
The clinical and pathological alterations in horses, experimentally inoculated with Bothropoides jararaca, Bothrops jararacussu, Bothrops moojeni and Bothropoides neuwiedi poisons, were studied with the purpose to supply subsidies for the diagnosis of the poisoning. The liofilized poisons were diluted in 1ml of physiologic solution and subcutaneously administered to six horses, at doses of 0.5 and 1mg/kg (B. jararaca), 0.8 and 1.6mg/kg (B. jararacussu), 0.205mg/kg (B. moojeni) and 1mg/kg (B. neuwiedi). All horses, less those that received the poison of B. jararacussu, died The clinical signs began between 8min and 2h10min after the inoculation. The clinical course varied, in the four cases of lethal exit, from 24h41min to 70h41min, and was 16 days in the two horses that recovered,. The clinical picture, independent of the poison type and doses, was characterized by tumefaction at the site of inoculation, dragging on the ground with the hooves of the inoculated leg, inquietude, apathy, decrease of reaction to external stimuli, pale mucous membranes and hemorrhages. Laboratory exams revealed normocytic normochrômic anemia with progressive decrease in the number of erythrocytes, of hemoglobin and of the hematocrit, and leucocytosis due to neutrophilia. There was increase of alamina aminotransferase, creatinaquinase, lactic dehydrogenase, ureia and glucose, as well increase of the time of activation of protrombin and partial tromboplastina. At postmortem examination, the main findings were extensive hemorrhagic areas in the subcutaneous tissue, with the presence of non-coagulated blood, to a large degree associataed with edema (hemorragic edema), which extended from the inoculation site of the venom to the cervical, thoraxic and scapular region, and to the leg. In the periphery of the hemorragic areas existed gelatinous edema. There were great amounts of sanguinary liquid in the thoracic, pericardic and abdominal cavities. No significant histological alterations were found.
Acesso aberto (Open Access)
Carcinoma de células escamosas perineal em cabras no Pará
(2009-05) BARBOSA NETO, José Diomedes; DUARTE, Marcos Dutra; OLIVEIRA, Carlos Magno Chaves; REIS, Alessandra dos Santos Belo; PEIXOTO, Tiago da Cunha; PEIXOTO, Paulo Fernando de Vargas; BRITO, Marilene de Farias
Squamous cell carcinoma in goats of two farms in the State of Pará is described. Prevalence, correlation with the pigmentation of the perineal area and macro and microscopic characteristics of the lesions were studied. The tumors on the perineum were of varied development degrees, diameters and forms. On one farm, in the county of Viseu, from a total of 347 goats, 20 presented the neoplasia (5.8%). On the second farm, in the county of Garrafão do Norte, three cases of squamous cell carcinoma in a herd of 400 goats (0.75%) were observed. The tumor was only observed in animals in which the perineal area was despigmented. The high incidence of this tumor appears to be due to lack of pigmentation at the períneo and the high and short tail of the goats, that exposes the area to intense ultraviolet radiation in the tropics.
Acesso aberto (Open Access)
Degenerative joint disease in cattle and buffaloes in the Amazon region: a retrospective study
(2014-09) BARBOSA NETO, José Diomedes; LIMA, Danillo Henrique da Silva; REIS, Alessandra dos Santos Belo; PINHEIRO, Cleyton Prado; SOUSA, Melina Garcia Saraiva de; SILVA, Jenevaldo Barbosa da; SALVARANI, Felipe Masiero; OLIVEIRA, Carlos Magno Chaves
A retrospective study of the epidemiological and clinic-pathological aspects of cattle and buffaloes with degenerative joint disease (DJD) was conducted in the state of Pará, Brazil. From 1999 to 2014, eleven cattle and 24 buffaloes were evaluated. All the treated animals with suspected DJD underwent a clinical examination of the musculoskeletal system. In seven cattle and eight buffaloes with clinical signs of the disease postmortem examination was performed. The common clinical signs observed in both species were chronic lameness, stiff gait, postural changes, audible crackles in the affected limb, prolonged recumbency, difficulty in getting up and progressive weight loss. The lesions observed at necropsy were: irregular articular surfaces, erosion of the articular cartilage and the underlying bone tissue, and proliferation of the periarticular bone tissue with formation of osteophytes. The most affected joints in cattle and buffaloes wereof the hind limb. In buffaloes, the main predisposing factor to the onset of DJD was phosphorus deficiency. In cattle, defects of the anatomical conformation of the hind limbs, chronic trauma due to the activities performed, such as semen collection, and advanced age possibly contributed to the emergence of the disease.
Acesso aberto (Open Access)
Dermatite por lambedura em bovinos no estado do Pará
(2011-02) BARBOSA NETO, José Diomedes; OLIVEIRA, Carlos Magno Chaves; ALBERNAZ, Tatiane Teles; SILVA, Natália da Silva e; SILVEIRA, José Alcides Sarmento da; REIS, Alessandra dos Santos Belo; SOUSA, Melina Garcia Saraiva de
A condition of 13 cattle from 10 farms in six districts in the state of Pará, Brazil, characterized by ulcerative skin wounds was studied. The lesions were observed in regions of the body to which the cattle had access with their own tongue; the animals licked the wounds frequently. Epidemiological studies, pathological findings and the differential diagnosis indicate that the dermatitis is caused by licking. The wounds healed after restraining the head of the animals that then were unable to lick themselves.
Acesso aberto (Open Access)
Detecção de Brucella abortus em linfonodos de búfalas (Bubalus bubalis) em diferentes fases da gestação
(Universidade Federal do Pará, 2015-12) SOUSA, Melina Garcia Saraiva de; BRITO, Marilene de Farias; UBIALI, Daniel Guimarães; FONSECA JÚNIOR, Antônio Augusto; SILVA, Jenevaldo Barbosa da; REIS, Alessandra dos Santos Belo; OLIVEIRA, Carlos Magno Chaves; BARBOSA NETO, José Diomedes
The objective of this study was to detect Brucella abortus in lymph nodes of buffaloes as well as to describe the lesions caused. Nineteen buffalo cows in various stages of pregnancy, serologically positive for brucellosis and subjected to culling were used. Fetal age was determined by ultrasound examination and the size of fetuses was measured at necropsy. Fragments of lymph nodes were collected for histopathology and qPCR. The detection of B. abortus DNA in the lymph nodes was checked from the fourth month of pregnancy in seven buffaloes and in a post-calving buffalo. Acute to chronic lymphadenitis was histologically diagnosed. B. abortus DNA was detected in all evaluated groups of lymph nodes; the mammary lymph nodes were the most affected.
Acesso aberto (Open Access)
Detecção e tratamento de otite por Rhabditis blumi em bovinos da região Norte do Brasil
(Universidade Federal do Pará, 2016-07) BARBOSA NETO, José Diomedes; SILVA, Jenevaldo Barbosa da; LIMA, Danillo Henrique da Silva; ARAÚJO, Luiz Henrique Vilela; SANTOS, Lívia Loiola dos; REIS, Alessandra dos Santos Belo; SALVARANI, Felipe Masiero; BRITO, Marilene de Farias
This study aimed to describe the occurrence of parasitic otitis caused by Rhabditis blumi in dairy cattle of the Gir race from a farm in northern Brazil. Forty-two samples were collected from cattle by swab washed from the external auditory canal (EAC). On clinical examination, in 71.4% (30/42) of the cattle the parasite was found in the cerumen of the ear canal, along with alopecia of head and hump caused by discomfort and itching of the auricular region. At microscopic analysis of material from the conjunctival sac the parasite was found in 90% (9/10) of the evaluated cattle. In addition, 9.5% (4/42) of the cattle showed nervous symptoms, such as mild to moderate rotation of the head, apathy, flaccid lips and unilateral ptosis, change in chewing and food accumulation in the oral cavity. Thirty cattle positive for Rhabditis spp. were randomly divided into three groups of 10 animals each: (G1) Cattle Control, (G2) Cattle treated with ivermectin 1% pour-on, and (G3) Cattle undergoing wash of the external auditory canal (EAC). Each treatment was repeated three times with intervals of seven days. In G1, 10 cattle remained infected throughout the study. In G2, 20% of the cattle were negative after the first two treatments, however were positive at the third evaluation. In G3, all cattle remained positive, but with decrease in parasite load. Identification by molecular analysis of amplified fragments through the expansion D2/D3 28S rDNA confirmed the presence of only Rhabditis blumi. Based on clinical, morphologic and molecular examination, it appears to be the first report of the occurrence of R. blumi infection in Gir cattle in the State of Pará, due to the purchase of cattle from areas where parasitic otitis has been diagnosed, as from Minas Gerais, to produce crossbred animals (Gir x Holstein). This emphasizes the importance of prior clinical examination by the veterinary service in order to transfer only healthy animals to other properties or regions. This appears also to be the first report on R. blumi infection of the conjunctival sac in cattle. Treatment with ivermectin in G2 did not produce clinical improvement.
Acesso aberto (Open Access)
Diagnóstico de paratuberculose por biópsia retal em búfalos
(2015-10) REIS, Alessandra dos Santos Belo; BRITO, Marilene de Farias; BEZERRA JÚNIOR, Pedro Soares; FONSECA JÚNIOR, Antônio Augusto; UBIALI, Daniel Guimarães; MOTA, Rinaldo Aparecido; LEITE, Rômulo Cerqueira; BARBOSA NETO, José Diomedes
Paratuberculosis in a herd of buffaloes was studied in the municipality of São Mateus, Maranhão, Brazil. Rectal biopsies were performed in 140 male and female Murrah, Mediterranean and crossbreed buffaloes older than 3 years. Postmortem examination of 11 buffaloes was performed to compare the rectal biopsies with possible lesions in mesenteric nodes and the intestine. The history of the herd and clinical examination revealed progressive weight loss and non-responsive antimicrobial diarrhea, dehydration and submandibular edema. Rectal biopsies showed in six buffaloes microscopically suggestive lesions for paratuberculosis through hematoxilin-eosin staining (HE), characterized by moderate multifocal granulomatous enteritis with epithelioid cell infiltration. In four buffaloes Langhans giant cells were found. In 15 buffaloes lymphocytic infiltrate was observed in the lamina propria of the large intestine. Ziehl-Neelsen staining (ZN) revealed in 4.3% (6/140) acid-fast bacilli in the rectal mucosa. Real time PCR amplified to 5.71% (7/140) Mycobacterium avium subsp. paratuberculosis (Map) DNA. 11 buffalos were submitted to postmortem examination, gross examination revealed augmented mesenteric nodes with whitish areas in the cut surface. The mucosa of the small intestine was irregular and thickened, with evident traverse folds and Peyer plates. The brownish intestinal content was fluid, the ileocecal valve area thickened and edematous with evident lymphatic vessels. Histological lesions in the mesenteric lymph node and small intestine four buffalo were compatible with those already described in the literature, and presented acid-fast bacilli by ZN staining and amplification of Map genetic material in qPCR. The concordance between the rectal biopsy and the postmortem samples was in agreement with the Kappa test (K=0.792) and was considered substantial or high. The rectal biopsy showed to be promising and can be used by practitioners, together with other techniques, for antemortem diagnosis in buffalo herds suspected to be affected by paratuberculosis. Samples obtained by rectal biopsy were suitable for ZN staining and real time PCR. Rectal biopsy can be used as a tool for diagnosis and control of Johne´s disease in buffaloes to select and eliminate positive animals within the herd and decrease gradually the spread of Map.
Acesso aberto (Open Access)
Estudo da paratuberculose em búfalos (Bubalus bubalis) no estado do Maranhão
(Universidade Federal do Pará, 2016-03-11) REIS, Alessandra dos Santos Belo; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557
This study investigated paratuberculosis (PTB) in water buffaloes in the state of Maranhão (MA). For that were performed using the rectal biopsy as an auxiliary tool in the collection of samples for ante mortem diagnosis of PTB; dosages of copper (Cu), zinc (Zn) and iron (Fe) in PTB positive animals was performed, as well as the report of the detection of Mycobacterium avium subsp. paratuberculosis (Map) in the utero and fetus of a buffalo. In the first study were conducted 140 rectal biopsies in buffaloes of Murrah, Mediterranean and their crossbreeds, over the age of three years in São Mateus, MA. These biopsies were processed by Ziehl-Neelsen (ZN) staining and quantitative PCR (qPCR). Postmortem examination of 11 buffaloes with clinical signs suggestive of PTB was performed and samples of ileum and mesenteric lymph nodes (MLN) were collected for performing qPCR and ZN. By ZN staining of rectal biopsies, 4.3% (6/140) acid-fast bacilli and qPCR, 5% (7/140) had amplification of genetic material. The pathological lesions of four animals were consistent with lesions suggestive of PTB showed acid-fast bacilli and amplification of genetic material. The correlation between rectal biopsy and analysis of ileal tissue and LM, according to the Kappa test was high (K=0.792). In the second study, 13 postmortem examination were carried in buffaloes of Murrah, Mediterranean and their crossbreeds, over the age of three years in the cities of São Mateus and São Luís, MA. Ileum and mesenteric lymph nodes samples were collected for Map search and liver to dosage minerals (Cu, Zn and Fe). Seven buffaloes were positive for PTB and allocated in Group 1; six buffaloes were negative and allocated in Group 2. In the dosing of trace minerals in Group 1 showed levels below the reference values for Cu and Zn. It was observed that the average Cu concentration was 18.0ppm and Zn 68.6ppm. In Group 2 the average Cu concentration was 113.7ppm and Zn 110.0ppm. The iron concentrations in both groups were high (>669ppm). The third study was conducted on a property in São Luís, MA. A buffalo cow pregnant with marked physical debility and clinical signs suggestive of PTB was eutanasiada and necropsiada. Fragments ileum, MLN, uterus, and placenta of buffalo cow were collected; fragments of kidney, liver, umbilical vessel and digestive system (DS) of the fetus were collected and were analyzed by Zn and qPCR. The buffalo cow was positive in ileum samples, mesenteric lymph nodes and uterus in qPCR and the fetus was positive in DS. In the ileum, MLN and feces were observed acid-fast bacilli. It is concluded that rectal biopsy analysis can be used to help diagnose ante mortem. The PTB could lead to mineral deficiency and worsen the clinical condition of the buffaloes. The presence of Map in buffalo can occur in various organs, including the reproductive system and intrauterine transmission can occur in this species.
Acesso aberto (Open Access)
Fotossensibilização em ovinos associada à ingestão de Brachiaria brizantha no estado do Pará
(2010-09) ALBERNAZ, Tatiane Teles; SILVEIRA, José Alcides Sarmento da; SILVA, Natália da Silva e; OLIVEIRA, Cairo Henrique Sousa de; REIS, Alessandra dos Santos Belo; OLIVEIRA, Carlos Magno Chaves; DUARTE, Marcos Dutra; BARBOSA NETO, José Diomedes
Two outbreaks of photosensitization by Brachiaria brizantha were studied and an experiment was performed in Santa Inês and Dorper crossbred sheep, two to three months old, on a farm in Santa Luzia do Pará municipality. These animals were kept from birth until about two months of age in a suspended stall floor, fed purple elephant grass (Pennisetum purpureum cv. purple), and had minerals and water ad libitum. After this period they were placed into a paddock of B. brizantha. At the time of the outbreaks and the experimental studies, the farm was visited for epidemiological assessment and clinical examination of the sheep, collection of blood samples for measurement of gamma glutamyltransferase, aspartate aminotransferase, conjugated bilirubin, unconjugated bilirubin, urea, and creatinine. Pasture samples were collected for saponin determination and count of Pithomyces chartarum spores. Necropsies with collection of material for histopathological studies were performed. Outbreak 1 occurred at the time of low rainfall, when the grass was scarce and mature, and morbidity and lethality was 43.4% and 81.6%, respectively. Outbreak 2 occurred at the beginning of the rainy season, with morbidity and lethality rates of 16.3% and 76.9%, respectively. From the 50 animals in the experiment, 10 received three times 200ml of rumen fluid taken from mother sheep of the same batch. The first of these administrations was given one day before the sheep were introduced into the pasture and the other two at weekly intervals. After 15 days in the pasture, the animals were unquiet, looked for shade, had edema of the ears, yellowish mucosae, were apathic, had anorexia and showed sloughing of the skin followed by crusting in some areas of the body. Both, the sheep of the outbreaks as those from the experiment, showed increased levels of GGT, AST, BD, BI, BT, urea and creatinine. In sheep which received ruminal fluid the values of urea and GGT were similar to those which did not receive the ruminal fluid. The creatinine, AST and bilirrubine values were lower in sheep that received the ruminal fluid. Two types of saponins, methylprotodioscin and protodioscin, were detected in the samples of B. brizantha from the outbreaks and the experiment. The level of saponins in Outbreak 1 and 2 was 0.92% and 0.88% respectively. The levels of saponins in the experiment ranged from 1.13% to 1.62%. The numbers of spores of Pithomyces chartarum in both outbreaks and in the experiment were negligible. At necropsy there was widespread jaundice, the liver was brown-yellow with marked lobular pattern and increased consistency and the kidneys were enlarged yellowish-green. Histological changes occurred mainly in the liver and consisted of mild proliferation of bile ducts in portal tracts, presence of binucleate hepatocytes, foamy macrophages, necrosis of isolated hepatocytes, cholangitis, presence of crystals in hepatocytes and macrophages.
Acesso aberto (Open Access)
Intoxicação natural por Ipomoea asarifolia (Convolvulaceae) em búfalos na Ilha de Marajó, Pará
(2012-09) BARBOSA NETO, José Diomedes; TOKARNIA, Carlos Maria Antônio Hubinger; ALBERNAZ, Tatiane Teles; OLIVEIRA, Carlos Magno Chaves; SILVA, Natália da Silva e; SILVEIRA, José Alcides Sarmento da; REIS, Alessandra dos Santos Belo; LIMA, Danillo Henrique da Silva
Epidemiological aspects and clinical signs of natural poisoning by Ipomoea asarifolia in buffaloes are described. The poisoning was diagnosed in four buffaloes on three different farms, in the County of Cachoeira do Arari, Marajó Island, Pará, and occurred in November-December, a period of drought and severe pasture shortage. The clinical signs were of the central nervous system, as unsteady gait, hypermetria, severe muscular tremors, falling down in unusual positions, nystagmus and marked excitement, signs that turned more severe after movement. Based on the epidemic aspects, clinical signs and absence of histopatological changes, poisoning by Ipomoea asarifolia was diagnosed.
Acesso aberto (Open Access)
Lesões de pele causadas pelos espinhos de Mimosa pudica (Leg. Mimosoideae) nos membros de bovinos e ovinos no estado do Pará
(2009-05) BARBOSA NETO, José Diomedes; SILVEIRA, José Alcides Sarmento da; ALBERNAZ, Tatiane Teles; SILVA, Natália da Silva e; REIS, Alessandra dos Santos Belo; OLIVEIRA, Carlos Magno Chaves; RIVERO, Gabriela Riet Correa; DUARTE, Marcos Dutra
A condition of cattle and sheep characterized by ulcerous and granulomatous skin lesions of the legs was studied. Epidemiological data and the nature of the lesions indicate that they are caused by the thorns of Mimosa pudica (Leg. Mimosoideae). The disease was observed only in pastures largely infested by the plant, and animals recover quickly when moved from them.
Acesso aberto (Open Access)
Lesões traumáticas de pele causadas pelos espinhos de Mimosa pudica e Mimosa debilis em equídeos
(2011-09) REIS, Alessandra dos Santos Belo; DUARTE, Marcos Dutra; SOUSA, Melina Garcia Saraiva de; FREITAS, Nayra Fernanda de Queiroz Ramos; YAMASAKI, Elise Miyuki; SILVA, André Guimarães Maciel e; OLIVEIRA, Carlos Magno Chaves; BARBOSA NETO, José Diomedes
Studies on the nature and cause of skin lesions in horses on a farm in the county of Castanhal, northeastern Para, Brazil were conducted. These were visits to the farm, epidemiological studies, blood sample collections, skin biopsies, and inspection of the pasture. The study included 25 Equidae, 14 males and 11 females, six months to eight years old. The animals showed ulcerative lesions of irregular borders on the head (nose, muzzle, upper and lower lips), in the oral cavity (buccal vestibule and gum) and on the limbs (billets, metacarpals and metatarsals and scapular-humeral joint). The histopathological examination revealed foci of cutaneous erosions with epidermal necrosis, spongiosis and vesicular degeneration of the remaining epidermis, and mild inflammatory infiltrate in the underlying dermis, consisting predominantly of macrophages and, to a lesser degree, eosinophils. The inspection of the pasture, which consisted of Brachiaria humidicola, revealed a heavy invasion by two plants provided with spines, Mimosa pudica and Mimosa debilis, of the Leguminosae Mimosoideae family. It was concluded that the skin lesions were caused by traumatic action of the spines of Mimosa pudica and Mimosa debilis.
Acesso aberto (Open Access)
Lesões traumáticas na pele causadas pelos espinhos de Mimosa pudica e Mimosa debilis em equídeos
(Universidade Federal do Pará, 2012-02-24) REIS, Alessandra dos Santos Belo; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557
We studied traumatic injury of the skin in horses caused by traumatic plants, popularly known as "sleep-mary", "Poppy", "scraper", "malice" and "do not touch me". The study was conducted on a farm in the district of Castanhal, northeastern of the state of Para, where there were technical visits, epidemiological study, blood samples, biopsies of affected skin and collection of plants. The study included 25 horses, 14 males and 11 females, aged between six months and eight years. The pasture consisted of Brachiaria humidicola and was heavily invaded by traumatizing plants. The animals showed ulcerative lesions of irregular borders, on the head (nose, muzzle, upper and lower lips and chamfer), oral cavity (buccal vestibule and gum) and limbs (billets, metacarpals and metatarsals and scapular-humeral joint). The histopathological examination revealed foci of cutaneous erosions, characterized by loss and epidermal necrosis with spongiosis and vesicular degeneration of the remaining epidermis and mild inflammatory infiltrate in the underlying dermis, consisting predominantly of macrophages and, to a lesser degree, eosinophils. We identified two plants, Mimosa pudica and Mimosa debilis, both from the Leguminosae Mimosoideae family. Based on these results we can conclude that the skin lesions were caused by the traumatic action of Mimosa pudica and Mimosa debilis.
Acesso aberto (Open Access)
Quadro clínico-patológico do envenenamento crotálico experimental em bubalinos comparado com o de bovinos
(2011-11) BARBOSA NETO, José Diomedes; SOUSA, Melina Garcia Saraiva de; TOKARNIA, Carlos Maria Antônio Hubinger; BRITO, Marilene de Farias; REIS, Alessandra dos Santos Belo; BOMJARDIM, Henrique dos Anjos; LOPES, Cinthia Távora de Albuquerque; OLIVEIRA, Carlos Magno Chaves
The objective of the study was to verify the sensibility of buffaloes to the poison of Cro- talus durissus terri icus and to study the clinical-pathological picture in buffaloes in com- parison with the one in cattle. The subcutaneous inoculation of the lio ilized poison of the snake, diluted in 1ml of physiologic solution, was done in the area of the humerus-radio- -ulnar joint of three buffaloes at doses of 0.015, 0.03 and 0.066mg/kg, and of two cattle at doses of 0.03 and 0.066mg/kg. The buffalo that received the 0.03mg/kg dose presented severe clinical signs but recovered six days later. The bovine that received the same dose, died after a clinical course of 22h56min. The 0.066mg/kg dose caused death of the bovine as also the buffalo, with a clinical course of 4h23min and 8h12min, respectively. The bu- ffalo that received the 0.015mg/kg dose recovered, after a course of 48 hours. The buffalo that died, showed clinical signs from 3h58min on, and the buffaloes that showed symp- toms from 17h25min and 24h00min after inoculation of the venom, but recovered. In the two cattle that died (with doses of 0.03 and 0.066mg/kg), the irst clinical signs occurred 6h10min and 6h31min after the inoculation of the venom. The inoculation produced in the buffaloes and cattle nervous symptoms of laccid paralysis. The main signs observed in the buffaloes as well as the cattle, were slight volume increase at the site of inoculation, respiration dif iculties characterized by predominantly abdominal breathing, apathy, sia- lorreia, dif iculty to get up when stimulated, evolution to sternal decubitus followed by lateral decubitus with peddling movements of the legs, and decrease of the re lexes related to the cranial nerves. The buffaloes showed also augmentation of the sustentation base, dragging of the hooves of the hind legs, slow and staggering gait, dif iculty in apprehension of the food, The cattle showed additionally paralysis of the eyeballs, revealed through non- -exhibition of the sclera during head rotation in latero-caudal direction. Laboratory exams revealed in the cattle and the buffaloes leucocytosis by neutro ilia, and in the biochemis- try series, increase in the levels of alanine aminotransferase, aspartato aminotransferase, creatinaquinase and lactic dehydrogenase. There was no alteration in the urinalysis nor in the activation time of protrombine and in the time of partially activated tromboplastin. Necropsy only evidenced slight edema corresponding to the inoculation site in one bovine. Histopathological examination revealed picnosis of the epitelial cell nuclei of some kidney tubules in the cortex (in the buffalo and in one bovine) and slight vacuolation of hepatocites (in one bovine).
Acesso aberto (Open Access)
Surto de encefalomielite equina Leste na Ilha de Marajó, Pará
(2013-04) CAMPOS, Karinny Ferreira; OLIVEIRA, Cairo Henrique Sousa de; REIS, Alessandra dos Santos Belo; YAMASAKI, Elise Miyuki; BRITO, Marilene de Farias; ANDRADE, Stefano Juliano Tavares de; DUARTE, Marcos Dutra; BARBOSA NETO, José Diomedes
Nine cases of equine encephalomyelitis on Marajó Island, state of Pará, Brazil, were studied. The affected horses had difficulty to stand, walked in circles, with marked depression, closed eyelids, tongue paralysis, muscle tremors, bruxism, anorexia and dehydration. Some had their ear and eyelid reflexes diminished, decreased tongue tone and tachycardia; laid down frequently they kept their head on the chest. Often they were seen resting their head on tree trunks or fences. At necropsy, hemorrhages of the meninges and spinal cord, and in some animals also adhesion of the meninges were found. Histologically there was diffuse encephalitis affecting mainly the gray matter, with meningitis and choroiditis. Presence of perivascular cuffs consisting of mononuclear inflammatory cells was observed. From two horses the Eastern equine encephalitis virus was identified by semi-nested reverse transcription polymerase chain reaction (semi-nested RT-PCR).