Navegando por Autor "RODRIGUES, Klebson de Jesus Araujo"

Agora exibindo 1 - 2 de 2

Acesso aberto (Open Access)
Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats
(Universidade Federal do Pará, 2007-10) FARO, Lilian Rosana Ferreira; RODRIGUES, Klebson de Jesus Araujo; SANTANA, Maxwell Barbosa de; VIDAL, Lucia; ALFONSO, M.
The present study was carried out in order to compare the effects of administration of organic (methylmercury, MeHg) and inorganic (mercury chloride, HgCl 2 ) forms of mercury on in vivo dopamine (DA) release from rat striatum. Experiments were performed in conscious and freely moving female adult Sprague-Dawley (230-280 g) rats using brain microdialysis coupled to HPLC with electrochemical detection. Perfusion of different concentrations of MeHg or HgCl 2 (2 µL/min for 1 h, N = 5-7/group) into the striatum produced significant increases in the levels of DA. Infusion of 40 µM, 400 µM, or 4 mM MeHg increased DA levels to 907 ± 31, 2324 ± 156, and 9032 ± 70% of basal levels, respectively. The same concentrations of HgCl 2 increased DA levels to 1240 ± 66, 2500 ± 424, and 2658 ± 337% of basal levels, respectively. These increases were associated with significant decreases in levels of dihydroxyphenylacetic acid and homovallinic acid. Intrastriatal administration of MeHg induced a sharp concentration-dependent increase in DA levels with a peak 30 min after injection, whereas HgCl 2 induced a gradual, lower (for 4 mM) and delayed increase in DA levels (75 min after the beginning of perfusion). Comparing the neurochemical profile of the two mercury derivatives to induce increases in DA levels, we observed that the time-course of these increases induced by both mercurials was different and the effect produced by HgCl 2 was not concentration-dependent (the effect was the same for the concentrations of 400 µM and 4 mM HgCl 2 ). These results indicate that HgCl 2 produces increases in extracellular DA levels by a mechanism differing from that of MeHg.
Acesso aberto (Open Access)
Plasticidade aumentada no córtex pré-frontal de ratos com a remoção de redes perineuronais
(Universidade Federal do Pará, 2016-11-01) RODRIGUES, Klebson de Jesus Araujo; BAHIA, Carlomagno Pacheco; http://lattes.cnpq.br/0910507988777644; PEREIRA JÚNIOR, Antônio; http://lattes.cnpq.br/1402289786010170
Aging is associated with decreasing brain plasticity, especially after the closure of the critical periods of plasticity (a sensitive limited period in life of elevated brain plasticity). Some brain regions may be particularly affected by aging, such as prefrontal cortex (PFC), which has a key role in the organization of higherorder cognitive aspects, the executive functions, including attention, set-shifting, working memory, decision making, etc. Decline of plasticity in the PFC and the brain is attributed mainly to the appearance of a structure called perineuronal net (PNNs) which enwraps the cell body and dendrites of many classes of neurons. PNNs are extracellular matrix structures consisting of chondroitin sulfate proteoglycans, hyaluronan, link proteins and tenascin, and are involved in the control of experience-dependent cortical plasticity and the closure of critical periods. Degradation of PNNs with the enzyme Chondroitinase ABC (ChABC) restores juvenile forms of plasticity in the adult brain. Here, we examined the developmental time course of PNN formation in the medial PFC (mPFC) of male rats ranging in age from the seventh postnatal day (PND) to 11 months. We used the lectin Vicia villosa agglutinin that binds to glycosaminoglycan chains present in the PNNs. We also investigated whether the digestion of PNNs by bilateral injection of ChABC in the mPFC may open a new window of increased plasticity in adult rats, evaluated by two executive function tests: object recognition and spontaneous alternation. We found that immature PNNs were observed in PND 20 animals, but mature PNNs were seen only after PND 75-90 and a mature form appeared around 5 months of age. In addition, our results showed that enzymatic PNN removal promoted a significant increase in performance in the ChABC-treated animals in both behavioral tests. The present study reveals for the first time the temporal development of PNN formation in the rat mPFC. We also show that the degradation of PNNs with ChABC not only promotes plasticity but also potentiates cognitive abilities in adult animals.