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Item Acesso aberto (Open Access) Complete genome sequence of Corynebacterium pseudotuberculosis biovar ovis strain P54B96 isolated from antelope in South Africa obtained by rapid next generation sequencing technology(BioMed Central, 2012) HASSAN, Syed Shah; GUIMARÃES, Luís Carlos; PEREIRA, Ulisses de Pádua; ISLAM, Arshad; ALI, Amjad; BAKHTIAR, Syeda Marriam; RIBEIRO, Dayana; SANTOS, Anderson Rodrigues dos; SOARES, Siomar de Castro; DORELLA, Fernanda Alves; GOMIDE, Anne Cybelle Pinto; SCHNEIDER, Maria Paula Cruz; BARBOSA, Maria Silvanira Ribeiro; ALMEIDA, Sintia Silva de; ABREU, Vinicius Augusto Carvalho de; ABURJAILE, Flavia Figueira; CARNEIRO, Adriana Ribeiro; CERDEIRA, Louise Teixeira; NASCIMENTO, Karina Kelly Fiaux do; BARBOSA, Eudes Guilherme Vieira; DINIZ, Carlos Augusto Almeida; ROCHA, Flávia de Souza; RAMOS, Rommel Thiago Juca; JAIN, Neha; TIWARI, Sandeep; BARH, Debmalya; MIYOSHI, Anderson; MÜLLER, Borna; SILVA, Artur Luiz da Costa da; AZEVEDO, Vasco Ariston de CarvalhoThe Actinobacteria, Corynebacterium pseudotuberculosis strain P54B96, a nonmotile, non sporulating and a mesophile bacterium, was isolated from liver, lung and mediastinal lymph node lesions in an antelope from South Africa. This strain is interesting in the sense that it has been found together with non-tuberculous mycobacteria (NTMs) which could nevertheless play a role in the lesion formation. In this work, we describe a set of features of C. pseudotuberculosis P54B96, together with the details of the complete genome sequence and annotation. The genome comprises of 2.34 Mbp long, single circular genome with 2,084 protein-coding genes, 12 rRNA, 49 tRNA and 62 pseudogenes and a G+C content of 52.19%. The analysis of the genome se quence provides means to better understanding the molecular and genetic basis of virulence of this bacterium, enabling a detailed investigation of its pathogenesis.Item Acesso aberto (Open Access) Complete genome sequence of Corynebacterium pseudotuberculosis I19, a strain isolated from a cow in Israel with bovine mastitis(American Society for Microbiology, 2011-01) SILVA, Artur Luiz da Costa da; SCHNEIDER, Maria Paula Cruz; CERDEIRA, Louise Teixeira; BARBOSA, Maria Silvanira Ribeiro; RAMOS, Rommel Thiago Juca; CARNEIRO, Adriana Ribeiro; OLIVEIRA, Rodrigo Santos de; LIMA, Margarida Maria Celeira de; CONCEIÇÃO, Marília Lima da; FERREIRA, Vívian D'Afonseca da Silva; ALMEIDA, Sintia Silva de; SANTOS, Anderson Rodrigues dos; SOARES, Siomar de Castro; GOMIDE, Anne Cybelle Pinto; ALI, Amjad; DORELLA, Fernanda Alves; ROCHA, Flávia de Souza; ABREU, Vinicius Augusto Carvalho de; TROST, Eva; TAUCH, Andreas; SHPIGEL, Nahum; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de CarvalhoThis work reports the completion and annotation of the genome sequence of Corynebacterium pseudotuberculosis I19, isolated from an Israeli dairy cow with severe clinical mastitis. To present the whole-genome sequence, a de novo assembly approach using 33 million short (25-bp) mate-paired SOLiD reads only was applied. Furthermore, the automatic, functional, and manual annotations were attained with the use of several algorithms in a multistep process.Item Acesso aberto (Open Access) Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain 1/06-A, Isolated from a Horse in North America(American Society for Microbiology, 2012-08) PETHICK, Florence E.; LAINSON, Alex F.; YAGA, Raja; FLOCKHART, Allen; SMITH, David G. E.; DONACHIE, Willie; CERDEIRA, Louise Teixeira; SILVA, Artur Luiz da Costa da; BOL, Erick Johny Maciel; LOPES, Thiago Souza; BARBOSA, Maria Silvanira Ribeiro; GOMIDE, Anne Cybelle Pinto; SANTOS, Anderson Rodrigues dos; SOARES, Siomar de Castro; ALMEIDA, Sintia Silva de; GUIMARÃES, Luís Carlos; ABURJAILE, Flavia Figueira; ABREU, Vinicius Augusto Carvalho de; RIBEIRO, Dayana; NASCIMENTO, Karina Kelly Fiaux do; DINIZ, Carlos Augusto Almeida; BARBOSA, Eudes Guilherme Vieira; PEREIRA, Ulisses de Pádua; HASSAN, Syed Shah; ALI, Amjad; BAKHTIAR, Syeda Marriam; DORELLA, Fernanda Alves; CARNEIRO, Adriana Ribeiro; RAMOS, Rommel Thiago Juca; ROCHA, Flávia de Souza; SCHNEIDER, Maria Paula Cruz; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de Carvalho; FONTAINE, Michael C.Corynebacterium pseudotuberculosis causes disease in several animal species, although distinct biovars exist that appear to be restricted to specific hosts. In order to facilitate a better understanding of the differences between biovars, we report here the complete genome sequence of the equine pathogen Corynebacterium pseudotuberculosis strain 1/06-A.Item Acesso aberto (Open Access) Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain Cp267, Isolated from a Llama(American Society for Microbiology, 2012-07) LOPES, Thiago Souza; SILVA, Artur Luiz da Costa da; RAMOS, Rommel Thiago Juca; CARNEIRO, Adriana Ribeiro; DORELLA, Fernanda Alves; ROCHA, Flávia de Souza; SANTOS, Anderson Rodrigues dos; LIMA, Alex Ranieri Jerônimo; GUIMARÃES, Luís Carlos; BARBOSA, Eudes Guilherme Vieira; RIBEIRO, Dayana; NASCIMENTO, Karina Kelly Fiaux do; DINIZ, Carlos Augusto Almeida; ABREU, Vinicius Augusto Carvalho de; ALMEIDA, Sintia Silva de; HASSAN, Syed Shah; ALI, Amjad; BAKHTIAR, Syeda Marriam; ABURJAILE, Flavia Figueira; GOMIDE, Anne Cybelle Pinto; SOARES, Siomar de Castro; PEREIRA, Ulisses de Pádua; SCHNEIDER, Maria Paula Cruz; MIYOSHI, Anderson; EDMAN, Judy; SPIER, Sharon; AZEVEDO, Vasco Ariston de CarvalhoIn this work we report the genome of Corynebacterium pseudotuberculosis strain 267, isolated from a llama. This pathogen is of great veterinary and economic importance, as it is the cause of caseous lymphadenitis in several livestock species around the world and causes significant losses due to the high cost of treatment.Item Acesso aberto (Open Access) Complete Genome Sequence of Type Strain Campylobacter fetus subsp. venerealis NCTC 10354(American Society for Microbiology, 2011-10) STYNEN, Ana Paula Reinato; LAGE, Andrey Pereira; MOORE, Robert J.; REZENDE, Antonio Mauro; FERREIRA, Vívian D'Afonseca da Silva; RUY, Patrícia de Cássia; OLIVEIRA, Nesley Jesus Daher de; RESENDE, Daniela de Melo; ALMEIDA, Sintia Silva de; SOARES, Siomar de Castro; ABREU, Vinicius Augusto Carvalho de; ROCHA, Aryane Aparecida Magalhães Cassiano; SANTOS, Anderson Rodrigues dos; BARBOSA, Eudes Guilherme Vieira; COUTO, Danielle Costa Carrara; DORELLA, Fernanda Alves; MIYOSHI, Anderson; LIMA, Alex Ranieri Jerônimo; CAMPOS, Frederico Davi da Silva; SÁ, Pablo Henrique Caracciolo Gomes de; LOPES, Thiago Souza; RODRIGUES, Ryan Maurício Araújo; CARNEIRO, Adriana Ribeiro; LEÃO, Tiago Ferreira; CERDEIRA, Louise Teixeira; RAMOS, Rommel Thiago Juca; SILVA, Artur Luiz da Costa da; AZEVEDO, Vasco Ariston de Carvalho; RUIZ, Jeronimo ConceiçãoCampylobacter fetus subsp. venerealis is the etiologic agent of bovine genital campylobacteriosis, a sexually transmitted disease of cattle that is of worldwide importance. The complete sequencing and annotation of the genome of the type strain C. fetus subsp. venerealis NCTC 10354T are reported.Item Acesso aberto (Open Access) Complete genome sequences of Corynebacterium pseudotuberculosis Strains 3/99-5 and 42/02-A, isolated from sheep in Scotland and Australia, respectively(American Society for Microbiology, 2012-09) PETHICK, Florence E.; LAINSON, Alex F.; YAGA, Raja; FLOCKHART, Allen; SMITH, David G. E.; DONACHIE, Willie; CERDEIRA, Louise Teixeira; SILVA, Artur Luiz da Costa da; BOL, Erick Johny Maciel; LOPES, Thiago Souza; BARBOSA, Maria Silvanira Ribeiro; GOMIDE, Anne Cybelle Pinto; SANTOS, Anderson Rodrigues dos; SOARES, Siomar de Castro; ALMEIDA, Sintia Silva de; GUIMARÃES, Luís Carlos; ABURJAILE, Flavia Figueira; ABREU, Vinicius Augusto Carvalho de; RIBEIRO, Dayana; NASCIMENTO, Karina Kelly Fiaux do; DINIZ, Carlos Augusto Almeida; BARBOSA, Eudes Guilherme Vieira; PEREIRA, Ulisses de Pádua; HASSAN, Syed Shah; ALI, Amjad; BAKHTIAR, Syeda Marriam; DORELLA, Fernanda Alves; CARNEIRO, Adriana Ribeiro; RAMOS, Rommel Thiago Juca; ROCHA, Flávia de Souza; SCHNEIDER, Maria Paula Cruz; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de Carvalho; FONTAINE, Michael C.Here, we report the whole-genome sequences of two ovine-pathogenic Corynebacterium pseudotuberculosisisolates: strain 3/99-5, which represents the first C. pseudotuberculosis genome originating from the United Kingdom, and 42/02-A, the second from Austra lia. These genome sequences will contribute to the objective of determining the global pan-genome of this bacterium.Item Acesso aberto (Open Access) Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains(Public Library of Science, 2011-04) RUIZ, Jeronimo Conceição; FERREIRA, Vívian D'Afonseca da Silva; SILVA, Artur Luiz da Costa da; ALI, Amjad; GOMIDE, Anne Cybelle Pinto; SANTOS, Anderson Rodrigues dos; ROCHA, Aryane Aparecida Magalhães Cassiano; DORELLA, Fernanda Alves; PACHECO, Luis Gustavo Carvalho; COSTA, Marcilia Pinheiro da; TURK, Meritxell Zurita; SEYFFERT, Núbia; MORAES, Pablo Matias Ribeiro de Oliveira; SOARES, Siomar de Castro; ALMEIDA, Sintia Silva de; CASTRO, Thiago Luiz de Paula; ABREU, Vinicius Augusto Carvalho de; TROST, Eva; BAUMBACH, Jan; TAUCH, Andreas; SCHNEIDER, Maria Paula Cruz; MCCULLOCH, John Anthony; CERDEIRA, Louise Teixeira; RAMOS, Rommel Thiago Juca; ZERLOTINI NETO, Adhemar; DOMINITINI, Anderson Joaquim; RESENDE, Daniela de Melo; COSER, Elisângela Monteiro; OLIVEIRA, Luciana Márcia de; PEDROSA, André Luiz; VIEIRA, Carlos Ueira; GUIMARÃES, Claudia Teixeira; BARTHOLOMEU, Daniela C.; OLIVEIRA, Diana Magalhães de; SANTOS, Fabrício Rodrigues dos; RABELO, Elida Mara Leite; LOBO, Francisco Pereira; FRANCO, Gloria Regina; COSTA, Ana Flávia Dias Vieira daCorynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity.Item Acesso aberto (Open Access) Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds(Public Library of Science, 2013-01) BARH, Debmalya; SHARMA, Neha Barve; GUPTA, Krishnakant; CHANDRA, Sudha; JAIN, Neha; TIWARI, Sandeep; LEON-SICAIROS, Nidia; CANIZALEZ-ROMAN, Adrian; SANTOS, Anderson Rodrigues dos; HASSAN, Syed Shah; ALMEIDA, Sintia Silva de; RAMOS, Rommel Thiago Juca; ABREU, Vinicius Augusto Carvalho de; CARNEIRO, Adriana Ribeiro; SOARES, Siomar de Castro; CASTRO, Thiago Luiz de Paula; MIYOSHI, Anderson; SILVA, Artur Luiz da Costa da; KUMAR, Anil; NARAYAN MISRA, Amarendra; BLUM, Kenneth; BRAVERMAN, Eric R.; AZEVEDO, Vasco Ariston de CarvalhoVibrio cholerae is the causal organism of the cholera epidemic, which is mostly prevalent in developing and underdeveloped countries. However, incidences of cholera in developed countries are also alarming. Because of the emergence of new drug resistant strains, even though several generic drugs and vaccines have been developed over time, Vibrio infections remain a global health problem that appeals for the development of novel drugs and vaccines against the pathogen. Here, applying comparative proteomic and reverse vaccinology approaches to the exoproteome and secretome of the pathogen, we have identified three candidate targets (ompU, uppP and yajC) for most of the pathogenic Vibrio strains. Two targets (uppP and yajC) are novel to Vibrio, and two targets (uppP and ompU) can be used to develop both drugs and vaccines (dual targets) against broad spectrum Vibrio serotypes. Using our novel computational approach, we have identified three peptide vaccine candidates that have high potential to induce both B- and T-cell-mediated immune responses from our identified two dual targets. These two targets were modeled and subjected to virtual screening against natural compounds derived from Piper betel. Seven compounds were identified first time from Piper betel to be highly effective to render the function of these targets to identify them as emerging potential drugs against Vibrio. Our preliminary validation suggests that these identified peptide vaccines and betel compounds are highly effective against Vibrio cholerae. Currently we are exhaustively validating these targets, candidate peptide vaccines, and betel derived lead compounds against a number of Vibrio species.Item Acesso aberto (Open Access) Genome sequence of Lactococcus lactis subsp. lactis NCDO 2118, a GABA - producing strain(American Society for Microbiology, 2014-10) SARAIVA, Tessália Diniz Luerce; SOARES, Siomar de Castro; RAMOS, Rommel Thiago Juca; SÁ, Pablo Henrique Caracciolo Gomes de; CARNEIRO, Adriana Ribeiro; MIRANDA, Fábio Malcher; FREIRA, Matheus; RENAN, Wendal; OLIVEIRA JÚNIOR, Alberto Fernandes de; SANTOS, Anderson Rodrigues dos; GOMIDE, Anne Cybelle Pinto; SOUZA, Bianca Mendes; CASTRO, Camila Prósperi de; DINIZ, Carlos Augusto Almeida; ROCHA, Clarissa Santos; MARIANO, Diego César Batista; AGUIAR, Edgar Lacerda de; FOLADOR, Edson Luiz; BARBOSA, Eudes Guilherme Vieira; ABURJAILE, Flavia Figueira; GONÇALVES, Lucas Amorim; GUIMARÃES, Luís Carlos; AZEVEDO, Marcela; AGRESTI, Pamela Mancha; SOUZA, Renata de Faria Silva; TIWARI, Sandeep; ALMEIDA, Sintia Silva de; HASSAN, Syed Shah; PEREIRA, Vanessa Bastos; ABREU, Vinicius Augusto Carvalho de; PEREIRA, Ulisses de Pádua; DORELLA, Fernanda Alves; CARVALHO, Alex Fiorini de; PEREIRA, Felipe Luiz; LEAL, Carlos Augusto Gomes; FIGUEIREDO, Henrique Cesar Pereira; SILVA, Artur Luiz da Costa da; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de CarvalhoLactococcus lactis subsp. lactis NCDO 2118 is a nondairy lactic acid bacterium, a xylose fermenter, and a gamma-aminobutyric acid (GABA) producer isolated from frozen peas. Here, we report the complete genome sequence of L. lactis NCDO 2118, a strain with probiotic potential activity.Item Acesso aberto (Open Access) Reannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria(Dove Medical Press, 2012-02) FERREIRA, Vívian D'Afonseca da Silva; SOARES, Siomar de Castro; ALI, Amjad; SANTOS, Anderson Rodrigues dos; GOMIDE, Anne Cybelle Pinto; ROCHA, Aryane Aparecida Magalhães Cassiano; FARIA, Cássio de Jesus; BARBOSA, Eudes Guilherme Vieira; GUIMARÃES, Luís Carlos; ESLABÃO, Marcus Redü; ALMEIDA, Sintia Silva de; ABREU, Vinicius Augusto Carvalho de; ZERLOTINI NETO, Adhemar; CARNEIRO, Adriana Ribeiro; CERDEIRA, Louise Teixeira; RAMOS, Rommel Thiago Juca; HIRATA JÚNIOR, Raphael; GUARALDI, Ana Luiza de Mattos; TROST, Eva; TAUCH, Andreas; SILVA, Artur Luiz da Costa da; SCHNEIDER, Maria Paula Cruz; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de CarvalhoBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism’s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other spe cies of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as “hypothetical proteins,” now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm. icb.ufmg.br/pub/C_diphtheriae_reannotation.embl.Item Acesso aberto (Open Access) The Corynebacterium pseudotuberculosis in silico predicted pan-exoproteome(BioMed Central Ltd, 2012) SANTOS, Anderson Rodrigues dos; CARNEIRO, Adriana Ribeiro; GALA-GARCÍA, Alfonso; GOMIDE, Anne Cybelle Pinto; BARH, Debmalya; BARBOSA, Eudes Guilherme Vieira; ABURJAILE, Flavia Figueira; DORELLA, Fernanda Alves; ROCHA, Flávia de Souza; GUIMARÃES, Luís Carlos; TURK, Meritxell Zurita; RAMOS, Rommel Thiago Juca; ALMEIDA, Sintia Silva de; SOARES, Siomar de Castro; PEREIRA, Ulisses de Pádua; ABREU, Vinicius Augusto Carvalho de; SILVA, Artur Luiz da Costa da; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de CarvalhoBackground: Pan-genomic studies aim, for instance, at defining the core, dispensable and unique genes within a species. A pan-genomics study for vaccine design tries to assess the best candidates for a vaccine against a specific pathogen. In this context, rather than studying genes predicted to be exported in a single genome, with pan genomics it is possible to study genes present in different strains within the same species, such as virulence factors. The target organism of this pan-genomic work here presented is Corynebacterium pseudotuberculosis, the etiologic agent of caseous lymphadenitis (CLA) in goat and sheep, which causes significant economic losses in those herds around the world. Currently, only a few antigens against CLA are known as being the basis of commercial and still ineffective vaccines. In this regard, the here presented work analyses, in silico, five C. pseudotuberculosis genomes and gathers data to predict common exported proteins in all five genomes. These candidates were also compared to two recent C. pseudotuberculosis in vitro exoproteome results. Results: The complete genome of five C. pseudotuberculosis strains (1002, C231, I19, FRC41 and PAT10) were submitted to pan-genomics analysis, yielding 306, 59 and 12 gene sets, respectively, representing the core, dispensable and unique in silico predicted exported pan-genomes. These sets bear 150 genes classified as secreted (SEC) and 227 as potentially surface exposed (PSE). Our findings suggest that the main C. pseudotuberculosis in vitro exoproteome could be greater, appended by a fraction of the 35 proteins formerly predicted as making part of the variant in vitro exoproteome. These genomes were manually curated for correct methionine initiation and redeposited with a total of 1885 homogenized genes. Conclusions: The in silico prediction of exported proteins has allowed to define a list of putative vaccine candidate genes present in all five complete C. pseudotuberculosis genomes. Moreover, it has also been possible to define the in silico predicted dispensable and unique C. pseudotuberculosis exported proteins. These results provide in silico evidence to further guide experiments in the areas of vaccines, diagnosis and drugs. The work here presented is the first whole C. pseudotuberculosis in silico predicted pan-exoproteome completed till today.Item Acesso aberto (Open Access) The Pan-Genome of the Animal Pathogen Corynebacterium pseudotuberculosis Reveals Differences in Genome Plasticity between the Biovar ovis and equi Strains(Public Library of Science, 2013-01) SOARES, Siomar de Castro; SILVA, Artur Luiz da Costa da; TROST, Eva; BLOM, Jochen; RAMOS, Rommel Thiago Juca; CARNEIRO, Adriana Ribeiro; ALI, Amjad; SANTOS, Anderson Rodrigues dos; GOMIDE, Anne Cybelle Pinto; DINIZ, Carlos Augusto Almeida; BARBOSA, Eudes Guilherme Vieira; DORELLA, Fernanda Alves; ABURJAILE, Flavia Figueira; ROCHA, Flávia de Souza; NASCIMENTO, Karina Kelly Fiaux do; GUIMARÃES, Luís Carlos; ALMEIDA, Sintia Silva de; HASSAN, Syed Shah; BAKHTIAR, Syeda Marriam; PEREIRA, Ulisses de Pádua; ABREU, Vinicius Augusto Carvalho de; SCHNEIDER, Maria Paula Cruz; MIYOSHI, Anderson; TAUCH, Andreas; AZEVEDO, Vasco Ariston de CarvalhoCorynebacterium pseudotuberculosis is a facultative intracellular pathogen and the causative agent of several infectious and contagious chronic diseases, including caseous lymphadenitis, ulcerative lymphangitis, mastitis, and edematous skin disease, in a broad spectrum of hosts. In addition, Corynebacterium pseudotuberculosis infections pose a rising worldwide economic problem in ruminants. The complete genome sequences of 15 C. pseudotuberculosis strains isolated from different hosts and countries were comparatively analyzed using a pan-genomic strategy. Phylogenomic, pan-genomic, core genomic, and singleton analyses revealed close relationships among pathogenic corynebacteria, the clonal-like behavior of C. pseudotuberculosis and slow increases in the sizes of pan-genomes. According to extrapolations based on the pan-genomes, core genomes and singletons, the C. pseudotuberculosis biovar ovis shows a more clonal-like behavior than the C. pseudotuberculosis biovar equi. Most of the variable genes of the biovar ovis strains were acquired in a block through horizontal gene transfer and are highly conserved, whereas the biovar equi strains contain great variability, both intra- and inter-biovar, in the 16 detected pathogenicity islands (PAIs). With respect to the gene content of the PAIs, the most interesting finding is the high similarity of the pilus genes in the biovar ovis strains compared with the great variability of these genes in the biovar equi strains. Concluding, the polymerization of complete pilus structures in biovar ovis could be responsible for a remarkable ability of these strains to spread throughout host tissues and penetrate cells to live intracellularly, in contrast with the biovar equi, which rarely attacks visceral organs. Intracellularly, the biovar ovis strains are expected to have less contact with other organisms than the biovar equi strains, thereby explaining the significant clonal-like behavior of the biovar ovis strains.Item Acesso aberto (Open Access) Whole-Genome Sequence of Corynebacterium pseudotuberculosis PAT10 Strain Isolated from Sheep in Patagonia, Argentina(American Society for Microbiology, 2011-11) CERDEIRA, Louise Teixeira; GOMIDE, Anne Cybelle Pinto; SCHNEIDER, Maria Paula Cruz; ALMEIDA, Sintia Silva de; SANTOS, Anderson Rodrigues dos; BARBOSA, Eudes Guilherme Vieira; ALI, Amjad; BARBOSA, Maria Silvanira Ribeiro; CARNEIRO, Adriana Ribeiro; RAMOS, Rommel Thiago Juca; OLIVEIRA, Rodrigo Santos de; BARH, Debmalya; SHARMA, Neha Barve; ZAMBARE, Vasudeo; BELCHIOR, Silvia Estevão; GUIMARÃES, Luís Carlos; SOARES, Siomar de Castro; DORELLA, Fernanda Alves; ROCHA, Flávia de Souza; ABREU, Vinicius Augusto Carvalho de; TAUCH, Andreas; TROST, Eva; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de Carvalho; SILVA, Artur Luiz da Costa daIn this work, we report the complete genome sequence of a Corynebacterium pseudotuberculosis PAT10 isolate, collected from a lung abscess in an Argentine sheep in Patagonia, whose pathogen also required an investigation of its pathogenesis. Thus, the analysis of the genome sequence offers a means to better understanding of the molecular and genetic basis of virulence of this bacterium.Item Acesso aberto (Open Access) Whole-Genome Sequence of Corynebacterium pseudotuberculosis Strain Cp162, Isolated from Camel(American Society for Microbiology, 2012) HASSAN, Syed Shah; SCHNEIDER, Maria Paula Cruz; RAMOS, Rommel Thiago Juca; CARNEIRO, Adriana Ribeiro; LIMA, Alex Ranieri Jerônimo; GUIMARÃES, Luís Carlos; ALI, Amjad; BAKHTIAR, Syeda Marriam; PEREIRA, Ulisses de Pádua; SANTOS, Anderson Rodrigues dos; SOARES, Siomar de Castro; DORELLA, Fernanda Alves; GOMIDE, Anne Cybelle Pinto; RIBEIRO, Dayana; BARBOSA, Maria Silvanira Ribeiro; ALMEIDA, Sintia Silva de; ABREU, Vinicius Augusto Carvalho de; ABURJAILE, Flavia Figueira; NASCIMENTO, Karina Kelly Fiaux do; BARBOSA, Eudes Guilherme Vieira; DINIZ, Carlos Augusto Almeida; ROCHA, Flávia de Souza; SAXENA, Rashmi; TIWARI, Sandeep; ZAMBARE, Vasudeo; GHOSH, Preetam; PACHECO, Luis Gustavo Carvalho; DOWSON, Christopher G.; KUMAR, Anil; BARH, Debmalya; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de Carvalho; SILVA, Artur Luiz da Costa daCorynebacterium pseudotuberculosis is a pathogen of great veterinary and economic importance, since it affects livestock, mainly sheep and goats, worldwide, together with reports of its presence in camels in several Arabic, Asiatic, and East and West African countries, as well as Australia. In this article, we report the genome sequence of Corynebacterium pseudotuberculosis strain Cp162, collected from the external neck abscess of a camel in the United Kingdom.